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Product Info Summary
| SKU: | A07317-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-RGS12 Antibody
SKU/Catalog Number
A07317-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-RGS12 Antibody catalog # A07317-1. Tested in WB, ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RGS12 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07317-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
1A3
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human RGS12. AA range:141-190
Reactive Species
A07317-1 is reactive to RGS12 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
156.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07317-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
ELISA 1:2000-20000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of 3T3 cells using RGS12 Polyclonal antibody.
Specific Publications For Anti-RGS12 Antibody (A07317-1)
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Customer Q&As
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3 Customer Q&As for Anti-RGS12 Antibody
Question
We are currently using anti-RGS12 antibody A07317-1 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
The anti-RGS12 antibody (A07317-1) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-25
Question
Would anti-RGS12 antibody A07317-1 work for WB with colon carcinoma?
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
According to the expression profile of colon carcinoma, RGS12 is highly expressed in colon carcinoma. So, it is likely that anti-RGS12 antibody A07317-1 will work for WB with colon carcinoma.
Boster Scientific Support
Answered: 2019-08-30
Question
My question regarding product A07317-1, anti-RGS12 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Mitchell
Verified customer
Asked: 2016-08-15
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07317-1 anti-RGS12 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-08-15
