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Product Info Summary
| SKU: | M00994-3 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-AMPK Alpha 1/PRKAA1 (Phospho-T183)/AMPK Alpha 2/PRKAA2 (Phospho-T172) Antibody (Monoclonal, 31P05)
View all PRKAA1/PRKAA2 Antibodies
SKU/Catalog Number
M00994-3
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-AMPK Alpha 1/PRKAA1 (Phospho-T183)/AMPK Alpha 2/PRKAA2 (Phospho-T172) Antibody (Monoclonal, 31P05) catalog # M00994-3. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AMPK Alpha 1/PRKAA1 (Phospho-T183)/AMPK Alpha 2/PRKAA2 (Phospho-T172) Antibody (Monoclonal, 31P05) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00994-3)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
31P05
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Thr183 of human AMPK alpha 1.
Reactive Species
M00994-3 is reactive to PRKAA1/PRKAA2 in Human, Mouse, Rat
Background of PRKAA1/PRKAA2
5'-AMP-activated protein kinase catalytic subunit alpha-1 is an enzyme that in humans is encoded by the PRKAA1 gene. The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00994-3 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 1:500-2000
Immunohistochemistry, 1:50-200
Validation Images & Assay Conditions
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Western blot analysis of AMPK Alpha 1/PRKAA1 using anti-AMPK Alpha 1/PRKAA1 antibody (M00994-3).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse brain tissue lysates,
Lane 2: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMPK Alpha 1/PRKAA1 antigen affinity purified monoclonal antibody (M00994-3) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for AMPK Alpha 1/PRKAA1 at approximately 64 kDa. The expected band size for AMPK Alpha 1/PRKAA1 is at 64 kDa.
Specific Publications For Anti-AMPK Alpha 1/PRKAA1 (Phospho-T183)/AMPK Alpha 2/PRKAA2 (Phospho-T172) Antibody (Monoclonal, 31P05) (M00994-3)
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