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Product Info Summary
| SKU: | M30391-1 |
|---|---|
| Size: | 20μL, 40μL, 100μL, 200μL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IP, IF, IHC, WB |
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Product info
Product Name
Anti-Cyclin B1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M30391-1
Size
20μL, 40μL, 100μL, 200μL
Description
The antibody detects endogenous levels of Cyclin B1 protein only when phosphorylated at Ser126. The antibody does not cross-react with Cyclin B1 phosphorylated at other sites. Validated for research with WB,IHC,IF,IP,ELISA applications. Anti Cyclin B1 is reactive for Human samples
Storage & Handling
Store at -20°C/1 year
Cite This Product
Anti-Cyclin B1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M30391-1)
Host
Rabbit
Contents
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% stabilizing protein
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Isotype
IgG, Kappa
Reactive Species
M30391-1 is reactive to CCNB in Human
Calculated molecular weight
48.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M30391-1 is guaranteed for ELISA, IP, IF, IHC, WB Boster Guarantee
Recommend Dilution
IHC 1:500-1:2000
WB 1:1000-1:5000
IF 1:200-1:1000
ELISA 1:5000-1:20000
IP 1:50-1:200
Validation Images & Assay Conditions
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Human tonsil was stained with anti-Cyclin B1 (phospho Ser126) rabbit antibody
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Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Cyclin B1 (phospho Ser126) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
Lane 1: HeLa
Lane 2: Jurkat
Predicted band size: 48kDa
Observed band size: 55kDa
Specific Publications For Anti-Cyclin B1 Rabbit Monoclonal Antibody (M30391-1)
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