AASDHPPT (NM_015423) Human Over-expression Lysate

Lysate of AASD-PPT over-expression cell lines, WB control

Transient overexpression lysate of aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase (AASDHPPT)

Product Info Summary

SKU: LS060496
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase (AASDHPPT)

Product Name

AASDHPPT (NM_015423) Human Over-expression Lysate

See all AASD-PPT products

SKU/Catalog Number

LS060496

Size

100 µg

Description

Transient overexpression lysate of aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase (AASDHPPT)

Storage & Handling

The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.

Cite This Product

AASDHPPT (NM_015423) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS060496)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for AASDHPPT (Source: Uniprot.org, NCBI)

Gene Name

AASDHPPT

Full Name

L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase

Weight

35.776kDa

Superfamily

P-Pant transferase superfamily

Alternative Names

4'-phosphopantetheinyl transferase; AASD-PPTDKFZp566E2346; Alpha-aminoadipic semialdehyde dehydrogenase-phosphopantetheinyl transferase; aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase; CGI-80; EC 2.7.8; EC 2.7.8.-; L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase; LYS2; LYS5 ortholog; LYS5 AASDHPPT AASD-PPT, ACPS, CGI-80, LYS2, LYS5 aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase|4-phosphopantetheinyl transferase|LYS5 ortholog|alpha-aminoadipic semialdehyde dehydrogenase-phosphopantetheinyl transferase|holo ACP synthase|holo-[acyl-carrier-protein] synthase

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on AASDHPPT, check out the AASDHPPT Infographic

AASDHPPT infographic

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In this infographic, you will see the following information for AASDHPPT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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