This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
HTR1A antibody (PA1647) performs well in IHC, WB and shows reactivity with Human, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 5 Publications
Filter:
IHC analysis of HTR1A using anti-HTR1A antibody (PA1647).
HTR1A was detected in a paraffin-embedded section of human endometrial adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HTR1A Antibody (PA1647) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
HTR1A was detected in a paraffin-embedded section of human endometrial adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HTR1A Antibody (PA1647) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Bundle Save
Anti-5HT1A Receptor/HTR1A Antibody Picoband®
HTR1A antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Rat | Rabbit | IHC, WB |
Tested Applications
HTR1A antibody was positively detected by WB in:
human hepatocellular carcinoma tumor tissue (HCCT), rat liver tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
HTR1A antibody was positively detected by IHC in:
human endometrial adenocarcinoma tissue, human lung cancer tissue, rat lymphaden tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human, Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
Filter:
Boster Guarantee:What is this?
WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Mouse
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-5HT1A Receptor/HTR1A Antibody Picoband®
View all HTR1A Antibodies
View all HTR1A Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human 5HT1A Receptor, different from the related rat and mouse sequences by one amino acid.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
46 kDa
Calculated Molecular Weight
46.1 kDa
Background of HTR1A
HTR1A (5-HYDROXYTRYPTAMINE RECEPTOR 1A), also called SEROTONIN 5-HT-1A RECEPTOR or BETA-2-ADRENERGIC RECEPTOR-LIKE PROTEIN G-21, is a subtype of 5-HT receptor that binds the endogenous neurotransmitter serotonin. It is a G protein-coupled receptor (GPCR) that is coupled to Gi/Go and mediates inhibitory neurotransmission. HTR1A denotes the human gene encoding for the receptor.The HTR1A gene is located at 5q12.3. The decreases in 5-HT-1A receptor densities correlated with decreased glucose utilization as measured by PET scan. Activation of 5-HT-1A receptors has been demonstrated to impair cognition, learning, and memory by inhibiting the release of glutamate and acetylcholine in various areas of the brain. 5-HT-1A receptors in the dorsal raphe nucleus are co-localized with neurokinin 1 (NK1) receptors and have been shown to inhibit the release of substance P, their endogenous ligand.
Cite This Product
Anti-5HT1A Receptor/HTR1A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1647)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Mouse
95%
High Homology
Rat
95%
High Homology
Rabbit
95%
High Homology
Cow
95%
High Homology
Sheep
95%
High Homology
Dog
84%
Moderate Homology
Zebrafish
84%
Moderate Homology
Drosophila
42%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for HTR1A antibody
Immunohistochemistry (IHC) protocol for HTR1A antibody
Publications
Application:
Species:
Activating SRC/MAPK signaling via 5-HT1A receptor contributes to the effect of vilazodone on improving thrombocytopenia
Published: 2024-04-04
Journal: eLife
Application: WB,IF
Impact Factor: 6.40
Species: Human
PMID: 38573820
Design, synthesis, and biological evaluation of piperazine derivatives involved in the 5-HT1AR/BDNF/PKA pathway
Published: 2023-12-11
Journal: JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
Application: WB
Impact Factor: 5.60
Species: Mouse
PMID: 38078358
Soporific effect of modified Suanzaoren Decoction on mice models of insomnia by regulating Orexin-A and HPA axis homeostasis
Published: 2021-09-10
Journal: BIOMEDICINE & PHARMACOTHERAPY
Application: IHC
Impact Factor: 6.53
Species: Mouse
PMID: 34509822
Role of cerebrospinal fluid-contacting nucleus in sodium sensing and sodium appetite
Published: 2015-04-21
Journal: PHYSIOLOGY & BEHAVIOR
Application: IHC
Impact Factor: 2.98
Species: Rat
PMID: 25911266
Effect of Monochromatic Light on Melatonin Secretion and Arylalkylamine N-Acetyltransferase mRNA Expression in the Retina and Pineal Gland of Broilers
Published: 2011-05-25
Journal: Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology
Application: IHC
Impact Factor: 1.40
Species: Chicken
PMID: 21618440
Review
Have you used Anti-5HT1A Receptor/HTR1A Antibody Picoband®?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-5HT1A Receptor/HTR1A Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
PA1647
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 12, 2026