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Product Info Summary
| SKU: | M03916 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-ADH5 Mouse Monoclonal Antibody [Clone ID: OTI5G4]
SKU/Catalog Number
M03916
Size
100 µl
Description
Boster Bio ADH5 mouse monoclonal antibody,clone OTI5G4. Catalog# M03916. Tested in IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-ADH5 Mouse Monoclonal Antibody [Clone ID: OTI5G4] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03916)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI5G4
Isotype
IgG1
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-266 of human ADH5 (NP_000662) produced in E.coli.
Reactive Species
M03916 is reactive to ADH5 in Human, Mouse, Rat
Calculated molecular weight
39.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03916 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:2000
IHC 1:150
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ADH5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ADH5 (1:2000).
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ADH5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-ADH5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ADH5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-ADH5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and ADH5-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with anti-ADH5 monoclonal antibody M03916 (1:500`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
Specific Publications For Anti-ADH5 Mouse Monoclonal Antibody [Clone ID: OTI5G4] (M03916)
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