Anti-Alcohol dehydrogenase class-3 ADH5 Antibody

alcohol dehydrogenase 5 antibody

Boster Bio Anti-Alcohol dehydrogenase class-3 ADH5 Antibody catalog # RP1111. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: RP1111
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, IHC, ICC, WB

Product Name

Anti-Alcohol dehydrogenase class-3 ADH5 Antibody

View all alcohol dehydrogenase 5 Antibodies

SKU/Catalog Number







Boster Bio Anti-Alcohol dehydrogenase class-3 ADH5 Antibody catalog # RP1111. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Alcohol dehydrogenase class-3 ADH5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1111)




Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.




E. coli-derived human ADH5 recombinant protein (Position: K212-I374). Human ADH5 shares 90.2% and 92% amino acid (aa) sequence identity with mouse and rat ADH5, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

RP1111 is reactive to ADH5 in Human, Mouse, Rat


RP1111 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Background of alcohol dehydrogenase 5

Alcohol dehydrogenase class-3 is an enzyme that in humans is encoded by the ADH5 gene. This gene encodes a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The encoded protein forms a homodimer. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis. The human genome contains several non-transcribed pseudogenes related to this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For ADH5 (Source: Uniprot.Org, NCBI)

Uniprot ID


Gene ID


Gene Name


Full Name

Alcohol dehydrogenase class-3




zinc-containing alcohol dehydrogenase family

Alternative Names

ADH-3; ADHXEC; alcohol dehydrogenase (class III), chi polypeptide; alcohol dehydrogenase 5 (class III), chi polypeptide; Alcohol dehydrogenase 5; Alcohol dehydrogenase class chi chain; alcohol dehydrogenase class-3; Alcohol dehydrogenase class-III; EC 1.1.1; EC 1.1.1.-; EC; FALDH; FDHGSNOR; formaldehyde dehydrogenase; Glutathione-dependent formaldehyde dehydrogenase; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase ADH5 ADH-3, ADHX, AMEDS, BMFS7, FALDH, FDH, GSH-FDH, GSNOR, HEL-S-60p alcohol dehydrogenase 5 (class III), chi polypeptide alcohol dehydrogenase class-3|S-(hydroxymethyl)glutathione dehydrogenase|S-nitrosoglutathione reductase|alcohol dehydrogenase (class III), chi polypeptide|alcohol dehydrogenase class chi chain|alcohol dehydrogenase class-III|epididymis secretory sperm binding protein Li 60p|formaldehyde dehydrogenase|glutathione-dependent formaldehyde dehydrogenase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ADH5, check out the ADH5 Infographic

ADH5 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ADH5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for RP1111

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4 Customer Q&As for Anti-Alcohol dehydrogenase class-3 ADH5 Antibody


We are currently using anti-ADH5 antibody RP1111 for human tissue, and we are satisfied with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-24


The anti-ADH5 antibody (RP1111) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-24


Do you have a BSA free version of anti-ADH5 antibody RP1111 available?

Verified Customer

Verified customer

Asked: 2019-05-30


Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-ADH5 antibody RP1111 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-05-30


I was wanting to use your anti-ADH5 antibody for IHC-P for rat lower esophagus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat lower esophagus identification?

Verified Customer

Verified customer

Asked: 2018-06-12


As indicated on the product datasheet, RP1111 anti-ADH5 antibody has been tested for IF, IHC-P, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat lower esophagus in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-06-12


See attached the WB image, lot number and protocol we used for lower esophagus using anti-ADH5 antibody RP1111. Please let me know if you require anything else.

R. Jones

Verified customer

Asked: 2017-05-15


Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-05-15


Total: $315


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