Product Info Summary
| SKU: | A11801 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-ADROPIN ENHO Antibody
SKU/Catalog Number
A11801
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ADROPIN ENHO Antibody (Catalog # A11801). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ADROPIN ENHO Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11801)
Host
Rabbit
Contents
ADROPIN antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ADROPIN antibody was raised against an 18 amino acid peptide near the carboxy terminus of human ADROPIN. The immunogen is located within the last 50 amino acids of ADROPIN.
Cross-reactivity
ADROPIN antibody is human, mouse and rat reactive. Despite its predicted molecular weight, ADROPIN often migrates at a higher than expected molecular weight in SDS-PAGE.
Reactive Species
A11801 is reactive to ENHO in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
7.9 kDa
Background of ENHO
ADROPIN is a recently identified protein that has been implicated in the maintenance of energy homeostasis and insulin resistance (1-3). ADROPIN expression is regulated by energy status and dietary nutrient content and is altered by obesity and regulates the expression of hepatic lipogenic genes and adipose tissue peroxisome proliferator-activated receptor gamma (PPAR-gamma) (1). ADROPIN levels increase with dietary fat content (2). ADROPIN has also been proposed to play a role in the regulation of endothelial function (3).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11801 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ADROPIN antibody can be used for detection of ADROPIN by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ADROPIN in human brain tissue lysate with ADROPIN antibody at 2 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of ADROPIN in human brain tissue with ADROPIN antibody at 5 μg/mL.
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Immunofluorescence of ADROPIN in human brain tissue with ADROPIN antibody at 20 μg/mL.
Specific Publications For Anti-ADROPIN ENHO Antibody (A11801)
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