Mouse FGF21 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

FGF-21 ELISA antibody pairs, Mouse, for 5 plates

Mouse FGF21 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Mouse FGF21 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Product Info Summary

SKU: EZ1379
Size: For 5 plates, 96 wells each
Reactive Species: Mouse
Application: ELISA

Product Name

Mouse FGF21 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

See all FGF-21 products

SKU/Catalog Number

EZ1379

Size

For 5 plates, 96 wells each

*Question: How many samples can I assay/run in this kit?

Description

Mouse FGF21 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Mouse FGF21 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse FGF21 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ1379)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A29-S210

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EZ1379 is reactive to FGF21 in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EZ1379 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog number Description Quantity Storage of opened/reconstituted material
EZ1379-CA Rabbit anti- mouse Fgf21 polyclonal antibody (Capture Antibody) 500 µl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit.
EZ1379-DA Biotinylated goat anti- mouse Fgf21 polyclonal antibody (Detection Antibody) 500 µl, 2.1 µg/mL (recommended dilution 1:100)
AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 µl (recommended dilution 1:100)
EZ1379-ST Lyophilized recombinant mouse Fgf21 standard 2 ng/tube×3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • 1. Microplate reader in standard size.
  • 2. Automated plate washer.
  • 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • 4. Clean tubes and Eppendorf tubes.
  • 5. 96 well microplate (Cat# AR1100)
  • 6. Plate Sealers.
  • 7. Capture Antibody Diluent: PBS.
  • 8. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
  • 9. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
  • 10. Stop Solution: 2 N H2SO4 (Cat# AR1105)
  • 11. Wash Buffer (PBS and PBS-T). Recipes:
    PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
    PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
  • *Item 5 – 11 are included in the EZ Set Accessory Kit (EZA001)

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0190.0930.1840.3220.5620.9961.6522.395

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)6432091865289938
Standard deviation2.812452.323.1227.1670.35
CV (%)4.4%7.5%5.7%4.8%9.4%7.5%

Gene/Protein Information For FGF21 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9JJN1

Gene ID

56636

Gene Name

FGF21

Full Name

Fibroblast growth factor 21

Weight

23.237kDa

Superfamily

heparin-binding growth factors family

Alternative Names

FGF21; FGF-21; fibroblast growth factor 21 Fgf21|Fgf8c|fibroblast growth factor 21|fibroblast growth factor 21|fibroblast growth factor 8c

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on FGF21, check out the FGF21 Infographic

FGF21 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for FGF21: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us suppo[email protected]

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1 Customer Q&As for Mouse FGF21 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Question

To Technical Team: I want to know if this EZ1379 Mouse FGF21 ELISA

Verified Customer

Verified customer

Asked: 2017-08-14

Answer

The product EZ1379 Mouse FGF21 ELISA Kit EZ-Set is suitable to use in serum samples that have been frozen, however we suggest not to mix samples that have been frozen with samples that have not frozen unless the impact of freezing is tested in a control experiment.

Boster Scientific Support

Answered: 2017-08-14

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