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Product Info Summary
| SKU: | A11242 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-AFAP1L AFAP1L1 Antibody
SKU/Catalog Number
A11242
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-AFAP1L AFAP1L1 Antibody (Catalog # A11242). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
AFAP1L antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-AFAP1L AFAP1L1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11242)
Host
Rabbit
Contents
AFAP1L Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
AFAP1L1 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human AFAP1L1. The immunogen is located within amino acids 70 - 120 of AFAP1L.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least four isoforms of AFAP1L1 are known to exist. This antibody is predicted to not cross-react with other AFAP family members.
Reactive Species
A11242 is reactive to Afap1l1 in Human, Mouse
Applications
A11242 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
86.638kDa
Background of Afap1l1
The actin filament-associated protein 1 (AFAP1)-like 1 protein is one of three members in the AFAP1 family of adaptor proteins. Like AFAP1, AFAP1L1 is an actin binding protein that has a role in actin cytoskeleton arrangement, but AFAP1L1 is thought to have a unique function distinct from AFAP1. AFAP1L1 colocalizes with cortactin and localizes to invadosomes, highly dynamic, actin-rich adhesion structures harboring metalloproteases, suggesting that AFAP1L1 affects invadosome formation (1, 4). Recent reports suggest that AFAP1L1 can be used as a prognostic marker for spindle cell sarcomas.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
AFAP1L1 antibody can be used for detection of AFAP1L1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of AFAP1L1 in A549 cell lysate with AFAP1L1 antibody at 1 μg/mL.
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Immunocytochemistry of AFAP1L in A549 cells with AFAP1L antibody at 5 μg/mL.
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Immunofluorescence of AFAP1L in A549 cells with AFAP1L antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For Afap1l1 (Source: Uniprot.org, NCBI)
Gene Name
Afap1l1
Full Name
Actin filament-associated protein 1-like 1
Weight
86.638kDa
Alternative Names
actin filament associated protein 1-like 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Afap1l1, check out the Afap1l1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Afap1l1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AFAP1L AFAP1L1 Antibody (A11242)
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