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Product Info Summary
| SKU: | A09513 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, ICC, WB |
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Product info
Product Name
Anti-ST3gal6 Antibody
SKU/Catalog Number
A09513
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ST3gal6 Antibody (Catalog # A09513). Tested in ELISA, WB, ICC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
ST3gal6 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ST3gal6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09513)
Host
Rabbit
Contents
ST3gal6 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ST3gal6 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ST3gal6. The immunogen is located within amino acids 230 - 280 of ST3gal6.
Reactive Species
A09513 is reactive to ST3GAL6 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
38.2 kDa
Background of ST3GAL6
Sialyltransferases catalyze the transfer of sialic acid from cytidine 5-prime monophospho-N-acetylneuraminic acid (CMP-NeuAc) to terminal positions of glycoprotein and glycolipid carbohydrate groups. Terminal NeuAc residues are key determinants of carbohydrate structures, such as the sialyl-Lewis X determinants, and are widely distributed in many cell types. However, cancer cells often express more heavily sialylated glycans on their cell surface and this feature sometimes correlates with invasiveness. In contrast, expression of ST3gal6, a member of the sialyltransferase family that sialylates type II lactosamine structures on glycoproteins and glycolipids, was found to be significantly decreased by hypermethylation of the gene in gastrointestinal cancer. At least three isoforms of ST3gal6 are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09513 is guaranteed for ELISA, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ST3gal6 antibody can be used for detection of ST3gal6 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ST3gal6 in HeLa cell lysate with ST3gal6 antibody at (A) 1 and (B) 2 μg/mL.
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Immunocytochemistry of ST3gal6 in HeLa cells with ST3gal6 antibody at 5 μg/mL.
Specific Publications For Anti-ST3gal6 Antibody (A09513)
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