Anti-alpha 1 Fetoprotein/AFP Antibody Picoband™

alpha-Fetoprotein/AFP antibody

Boster Bio Anti-alpha 1 Fetoprotein/AFP Antibody Picoband™ catalog # PB9090. Tested in IHC, WB applications. This antibody reacts with Human. Cited in 10 publication(s).

Product Info Summary

SKU: PB9090
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

Product Name

Anti-alpha 1 Fetoprotein/AFP Antibody Picoband™

View all alpha-Fetoprotein/AFP Antibodies

SKU/Catalog Number

PB9090

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-alpha 1 Fetoprotein/AFP Antibody Picoband™ catalog # PB9090. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-alpha 1 Fetoprotein/AFP Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9090)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human AFP recombinant protein (Position: Q378-V609). Human AFP shares 71% and 73% amino acid (aa) sequences identity with mouse and rat AFP, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9090 is reactive to AFP in Human

Applications

PB9090 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

69 kDa

Calculated molecular weight

68.678kDa

Background of alpha-Fetoprotein/AFP

AFP, also called Alpha-fetoprotein; alpha-fetoprotein, is a protein that in humans is encoded by the AFP gene. It is mapped to 4q13.3. The level of AFP in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. In rodents AFP binds estradiol to prevent the transport of this hormone across the placenta to the fetus. The main function of this is to prevent the virilization of female fetuses. Moreover, it has an important role as a diagnostic marker, especially in certain tumors and liver diseases of childhood. AFP is also used to test the potential usefulness of plasma alpha fetoprotein determination as a diagnostic marker in biliary atresia, hepatitis, and yolk sac derived tumours.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat

Validation Images & Assay Conditions

Gene/Protein Information For AFP (Source: Uniprot.org, NCBI)

Gene Name

AFP

Full Name

Alpha-fetoprotein

Weight

68.678kDa

Superfamily

ALB/AFP/VDB family

Alternative Names

AFP; Alpha-1-fetoprotein; alpha-fetoglobulin; alpha-Fetoprotein; DSCAM2; FETA; HP; HPAFP AFP AFPD, FETA, HPAFP alpha fetoprotein alpha-fetoprotein|alpha-1-fetoprotein|alpha-fetoglobulin

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on AFP, check out the AFP Infographic

AFP infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for AFP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB9090 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Selective tropism of liver stem cells to hepatocellular carcinoma in vivo.

Adipose tissue-derived mesenchymal stem cells differentiated into hepatocyte-like cellsin vivoandin vitro

Extracellular regulated protein kinases 1/2 phosphorylation is required for hepatic differentiation of human umbilical cord-derived mesenchymal stem cells

Shan Zy, Wu Ys, Li X, Shen Xh, Wang Zd, Liu Zh, Shen Jl, Lei L. Cell Reprogram. 2014 Feb;16(1):77-83. Doi: 10.1089/Cell.2013.0067. Epub 2014 Jan 4. Continuous Passages Accelerate The Reprogramming Of Mouse Induced Pluripotent Stem Cells.

Su Z, Wu R, Tan Z, Li Y, Chen L, Luo J, Zhang M. Biochem Biophys Res Commun. 2010 Apr 9;394(3):616-22. Doi: 10.1016/J.Bbrc.2010.03.033. Epub 2010 Mar 7. Early Homing Behavior Of Stro-1- Mesenchyme-Like Cells Derived From Human Embryonic Stem Cells...

Yin L, Zhu Y, Yang J, Ni Y, Zhou Z, Chen Y, Wen L. Mol Med Rep. 2015 Mar;11(3):1722-32. Doi: 10.3892/Mmr.2014.2935. Epub 2014 Nov 13. Adipose Tissue-Derived Mesenchymal Stem Cells Differentiated Into Hepatocyte-Like Cells In Vivo And In Vitro.

Li Y, Wu Z, Liu Z. Analyst. 2015 Jun 21;140(12):4083-8. Doi: 10.1039/C5An00357A. Epub 2015 Apr 17. An Immune Sandwich Assay Of Carcinoembryonic Antigen Based On The Joint Use Of Upconversion Phosphors And Magnetic Beads.

Wu R, Gu B, Zhao X, Tan Z, Chen L, Zhu J, Zhang M. Hum Cell. 2013 Mar;26(1):19-27. Doi: 10.1007/S13577-011-0022-3. Epub 2011 Jun 15. Derivation Of Multipotent Nestin(+)/Cd271 (-)/Stro-1 (-) Mesenchymal-Like Precursors From Human Embryonic Stem Cel...

Wang Q, Xu Y, Zhou W, Zhong L, Wen Z, Yu H, Chen S, Shen J, Chen H, She Q, Jiang J, Miao J, Wei W. Int J Biol Sci. 2014 Nov 18;10(10):1181-92. Doi: 10.7150/Ijbs.10275. Ecollection 2014. The Viral Oncoprotein Hbx Of Hepatitis B Virus Promotes The G...

Qin Xg, Hua Z, Shuang W, Wang Yh, Cui Yd. Pharm Biol. 2010 Mar;48(3):275-81. Doi: 10.3109/13880200903104101. Effects Of Matrine On Hepg2 Cell Proliferation And Expression Of Tumor Relevant Proteins In Vitro.

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16 Customer Q&As for Anti-alpha 1 Fetoprotein/AFP Antibody Picoband™

Question

I would like using your anti-alpha 1 Fetoprotein/AFP antibody for regulation of insulin-like growth factor (igf) transport and uptake by insulin-like growth factor binding proteins (igfbps) studies. Has this antibody been tested with western blotting on hepg2 whole cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-05-08

Answer

Thanks for your inquiry. This PB9090 anti-alpha 1 Fetoprotein/AFP antibody is tested on hepg2 whole cell lysate. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-05-08

Question

We need to test anti-alpha 1 Fetoprotein/AFP antibody PB9090 on mouse embryo for research purposes, then I may be interested in using anti-alpha 1 Fetoprotein/AFP antibody PB9090 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-05-06

Answer

The products we sell, including anti-alpha 1 Fetoprotein/AFP antibody PB9090, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-05-06

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for embryo using anti-alpha 1 Fetoprotein/AFP antibody PB9090. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-04-23

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-23

Question

Is this PB9090 anti-alpha 1 Fetoprotein/AFP antibody reactive to the isotypes of AFP?

Verified Customer

Verified customer

Asked: 2020-03-06

Answer

The immunogen of PB9090 anti-alpha 1 Fetoprotein/AFP antibody is E.coli-derived human AFP recombinant protein (Position: Q378-V609). Human AFP shares 71% and 73% amino acid (aa) sequences identity with mouse and rat AFP, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-03-06

Question

Can you help my question with product PB9090, anti-alpha 1 Fetoprotein/AFP antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-10-31

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9090 anti-alpha 1 Fetoprotein/AFP antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-10-31

Question

We are currently using anti-alpha 1 Fetoprotein/AFP antibody PB9090 for mouse tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2019-10-16

Answer

The anti-alpha 1 Fetoprotein/AFP antibody (PB9090) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-10-16

Question

We have seen staining in human lung. Are there any suggestions? Is anti-alpha 1 Fetoprotein/AFP antibody supposed to stain lung positively?

Verified Customer

Verified customer

Asked: 2019-08-15

Answer

From literature lung does express AFP. From Uniprot.org, AFP is expressed in embryo, heart, lung, brain cajal-retzius cell, among other tissues. Regarding which tissues have AFP expression, here are a few articles citing expression in various tissues:
Brain, and Cajal-Retzius cell, Pubmed ID: 6187626
Heart, Pubmed ID: 14702039
Lung, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-08-15

Question

Does anti-alpha 1 Fetoprotein/AFP antibody PB9090 work for IHC with embryo?

Verified Customer

Verified customer

Asked: 2018-06-13

Answer

According to the expression profile of embryo, AFP is highly expressed in embryo. So, it is likely that anti-alpha 1 Fetoprotein/AFP antibody PB9090 will work for IHC with embryo.

Boster Scientific Support

Answered: 2018-06-13

Question

I was wanting to use your anti-alpha 1 Fetoprotein/AFP antibody for IHC for mouse embryo on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse embryo identification?

Verified Customer

Verified customer

Asked: 2018-05-08

Answer

It shows on the product datasheet, PB9090 anti-alpha 1 Fetoprotein/AFP antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse embryo in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-05-08

Question

Will PB9090 anti-alpha 1 Fetoprotein/AFP antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-04-30

Answer

As indicated on the product datasheet, PB9090 anti-alpha 1 Fetoprotein/AFP antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-04-30

Question

Is a blocking peptide available for product anti-alpha 1 Fetoprotein/AFP antibody (PB9090)?

Verified Customer

Verified customer

Asked: 2018-03-14

Answer

We do provide the blocking peptide for product anti-alpha 1 Fetoprotein/AFP antibody (PB9090). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-03-14

Question

I see that the anti-alpha 1 Fetoprotein/AFP antibody PB9090 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2017-06-09

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-06-09

Question

Do you have a BSA free version of anti-alpha 1 Fetoprotein/AFP antibody PB9090 available?

R. Thomas

Verified customer

Asked: 2016-12-07

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-alpha 1 Fetoprotein/AFP antibody PB9090 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2016-12-07

Question

Our team were satisfied with the WB result of your anti-alpha 1 Fetoprotein/AFP antibody. However we have been able to see positive staining in embryo secreted. using this antibody. Is that expected? Could you tell me where is AFP supposed to be expressed?

P. Miller

Verified customer

Asked: 2014-07-04

Answer

Based on literature, embryo does express AFP. Generally AFP expresses in secreted. Regarding which tissues have AFP expression, here are a few articles citing expression in various tissues:
Brain, and Cajal-Retzius cell, Pubmed ID: 6187626
Heart, Pubmed ID: 14702039
Lung, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2014-07-04

Question

We have tried in the past anti-alpha 1 Fetoprotein/AFP antibody for IHC on heart last year. I am using mouse, and We intend to use the antibody for WB next. I am interested in examining heart as well as embryo in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

A. Parker

Verified customer

Asked: 2014-06-12

Answer

I viewed the website and datasheets of our anti-alpha 1 Fetoprotein/AFP antibody and I see that PB9090 has been tested on mouse in both IHC and WB. Thus PB9090 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2014-06-12

Question

See attached the WB image, lot number and protocol we used for embryo using anti-alpha 1 Fetoprotein/AFP antibody PB9090. Please let me know if you require anything else.

V. Krishna

Verified customer

Asked: 2014-04-08

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-04-08

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Total: $370

SKU:PB9090

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