Product Info Summary
SKU: | PB9090 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-alpha 1 Fetoprotein/AFP Antibody Picoband™
View all alpha-Fetoprotein/AFP Antibodies
SKU/Catalog Number
PB9090
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-alpha 1 Fetoprotein/AFP Antibody Picoband™ catalog # PB9090. Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-alpha 1 Fetoprotein/AFP Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9090)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human AFP recombinant protein (Position: Q378-V609). Human AFP shares 71% and 73% amino acid (aa) sequences identity with mouse and rat AFP, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9090 is reactive to AFP in Human
Applications
PB9090 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
69 kDa
Calculated molecular weight
68.678kDa
Background of alpha-Fetoprotein/AFP
AFP, also called Alpha-fetoprotein; alpha-fetoprotein, is a protein that in humans is encoded by the AFP gene. It is mapped to 4q13.3. The level of AFP in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. In rodents AFP binds estradiol to prevent the transport of this hormone across the placenta to the fetus. The main function of this is to prevent the virilization of female fetuses. Moreover, it has an important role as a diagnostic marker, especially in certain tumors and liver diseases of childhood. AFP is also used to test the potential usefulness of plasma alpha fetoprotein determination as a diagnostic marker in biliary atresia, hepatitis, and yolk sac derived tumours.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of AFP using anti-AFP antibody (PB9090).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human hepatocellular carcinoma tumor tissue (HCCT) lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AFP antigen affinity purified polyclonal antibody (Catalog # PB9090) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AFP at approximately 69 kDa. The expected band size for AFP is at 69 kDa.
Click image to see more details
Figure 2. IHC analysis of AFP using anti-AFP antibody (PB9090).
AFP was detected in paraffin-embedded section of human liver cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AFP Antibody (PB9090) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For AFP (Source: Uniprot.org, NCBI)
Gene Name
AFP
Full Name
Alpha-fetoprotein
Weight
68.678kDa
Superfamily
ALB/AFP/VDB family
Alternative Names
AFP; Alpha-1-fetoprotein; alpha-fetoglobulin; alpha-Fetoprotein; DSCAM2; FETA; HP; HPAFP AFP AFPD, FETA, HPAFP alpha fetoprotein alpha-fetoprotein|alpha-1-fetoprotein|alpha-fetoglobulin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AFP, check out the AFP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AFP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-alpha 1 Fetoprotein/AFP Antibody Picoband™ (PB9090)
Hello CJ!
PB9090 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Selective tropism of liver stem cells to hepatocellular carcinoma in vivo.
Adipose tissue-derived mesenchymal stem cells differentiated into hepatocyte-like cellsin vivoandin vitro
Extracellular regulated protein kinases 1/2 phosphorylation is required for hepatic differentiation of human umbilical cord-derived mesenchymal stem cells
Shan Zy, Wu Ys, Li X, Shen Xh, Wang Zd, Liu Zh, Shen Jl, Lei L. Cell Reprogram. 2014 Feb;16(1):77-83. Doi: 10.1089/Cell.2013.0067. Epub 2014 Jan 4. Continuous Passages Accelerate The Reprogramming Of Mouse Induced Pluripotent Stem Cells.
Su Z, Wu R, Tan Z, Li Y, Chen L, Luo J, Zhang M. Biochem Biophys Res Commun. 2010 Apr 9;394(3):616-22. Doi: 10.1016/J.Bbrc.2010.03.033. Epub 2010 Mar 7. Early Homing Behavior Of Stro-1- Mesenchyme-Like Cells Derived From Human Embryonic Stem Cells...
Yin L, Zhu Y, Yang J, Ni Y, Zhou Z, Chen Y, Wen L. Mol Med Rep. 2015 Mar;11(3):1722-32. Doi: 10.3892/Mmr.2014.2935. Epub 2014 Nov 13. Adipose Tissue-Derived Mesenchymal Stem Cells Differentiated Into Hepatocyte-Like Cells In Vivo And In Vitro.
Li Y, Wu Z, Liu Z. Analyst. 2015 Jun 21;140(12):4083-8. Doi: 10.1039/C5An00357A. Epub 2015 Apr 17. An Immune Sandwich Assay Of Carcinoembryonic Antigen Based On The Joint Use Of Upconversion Phosphors And Magnetic Beads.
Wu R, Gu B, Zhao X, Tan Z, Chen L, Zhu J, Zhang M. Hum Cell. 2013 Mar;26(1):19-27. Doi: 10.1007/S13577-011-0022-3. Epub 2011 Jun 15. Derivation Of Multipotent Nestin(+)/Cd271 (-)/Stro-1 (-) Mesenchymal-Like Precursors From Human Embryonic Stem Cel...
Wang Q, Xu Y, Zhou W, Zhong L, Wen Z, Yu H, Chen S, Shen J, Chen H, She Q, Jiang J, Miao J, Wei W. Int J Biol Sci. 2014 Nov 18;10(10):1181-92. Doi: 10.7150/Ijbs.10275. Ecollection 2014. The Viral Oncoprotein Hbx Of Hepatitis B Virus Promotes The G...
Qin Xg, Hua Z, Shuang W, Wang Yh, Cui Yd. Pharm Biol. 2010 Mar;48(3):275-81. Doi: 10.3109/13880200903104101. Effects Of Matrine On Hepg2 Cell Proliferation And Expression Of Tumor Relevant Proteins In Vitro.
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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16 Customer Q&As for Anti-alpha 1 Fetoprotein/AFP Antibody Picoband™
Question
I would like using your anti-alpha 1 Fetoprotein/AFP antibody for regulation of insulin-like growth factor (igf) transport and uptake by insulin-like growth factor binding proteins (igfbps) studies. Has this antibody been tested with western blotting on hepg2 whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-05-08
Answer
Thanks for your inquiry. This PB9090 anti-alpha 1 Fetoprotein/AFP antibody is tested on hepg2 whole cell lysate. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-05-08
Question
We need to test anti-alpha 1 Fetoprotein/AFP antibody PB9090 on mouse embryo for research purposes, then I may be interested in using anti-alpha 1 Fetoprotein/AFP antibody PB9090 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
The products we sell, including anti-alpha 1 Fetoprotein/AFP antibody PB9090, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-05-06
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for embryo using anti-alpha 1 Fetoprotein/AFP antibody PB9090. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-23
Question
Is this PB9090 anti-alpha 1 Fetoprotein/AFP antibody reactive to the isotypes of AFP?
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
The immunogen of PB9090 anti-alpha 1 Fetoprotein/AFP antibody is E.coli-derived human AFP recombinant protein (Position: Q378-V609). Human AFP shares 71% and 73% amino acid (aa) sequences identity with mouse and rat AFP, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-06
Question
Can you help my question with product PB9090, anti-alpha 1 Fetoprotein/AFP antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9090 anti-alpha 1 Fetoprotein/AFP antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-31
Question
We are currently using anti-alpha 1 Fetoprotein/AFP antibody PB9090 for mouse tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
The anti-alpha 1 Fetoprotein/AFP antibody (PB9090) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-16
Question
We have seen staining in human lung. Are there any suggestions? Is anti-alpha 1 Fetoprotein/AFP antibody supposed to stain lung positively?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
From literature lung does express AFP. From Uniprot.org, AFP is expressed in embryo, heart, lung, brain cajal-retzius cell, among other tissues. Regarding which tissues have AFP expression, here are a few articles citing expression in various tissues:
Brain, and Cajal-Retzius cell, Pubmed ID: 6187626
Heart, Pubmed ID: 14702039
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-08-15
Question
Does anti-alpha 1 Fetoprotein/AFP antibody PB9090 work for IHC with embryo?
Verified Customer
Verified customer
Asked: 2018-06-13
Answer
According to the expression profile of embryo, AFP is highly expressed in embryo. So, it is likely that anti-alpha 1 Fetoprotein/AFP antibody PB9090 will work for IHC with embryo.
Boster Scientific Support
Answered: 2018-06-13
Question
I was wanting to use your anti-alpha 1 Fetoprotein/AFP antibody for IHC for mouse embryo on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse embryo identification?
Verified Customer
Verified customer
Asked: 2018-05-08
Answer
It shows on the product datasheet, PB9090 anti-alpha 1 Fetoprotein/AFP antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse embryo in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-05-08
Question
Will PB9090 anti-alpha 1 Fetoprotein/AFP antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-04-30
Answer
As indicated on the product datasheet, PB9090 anti-alpha 1 Fetoprotein/AFP antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-04-30
Question
Is a blocking peptide available for product anti-alpha 1 Fetoprotein/AFP antibody (PB9090)?
Verified Customer
Verified customer
Asked: 2018-03-14
Answer
We do provide the blocking peptide for product anti-alpha 1 Fetoprotein/AFP antibody (PB9090). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-03-14
Question
I see that the anti-alpha 1 Fetoprotein/AFP antibody PB9090 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-06-09
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-06-09
Question
Do you have a BSA free version of anti-alpha 1 Fetoprotein/AFP antibody PB9090 available?
R. Thomas
Verified customer
Asked: 2016-12-07
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-alpha 1 Fetoprotein/AFP antibody PB9090 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-12-07
Question
Our team were satisfied with the WB result of your anti-alpha 1 Fetoprotein/AFP antibody. However we have been able to see positive staining in embryo secreted. using this antibody. Is that expected? Could you tell me where is AFP supposed to be expressed?
P. Miller
Verified customer
Asked: 2014-07-04
Answer
Based on literature, embryo does express AFP. Generally AFP expresses in secreted. Regarding which tissues have AFP expression, here are a few articles citing expression in various tissues:
Brain, and Cajal-Retzius cell, Pubmed ID: 6187626
Heart, Pubmed ID: 14702039
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2014-07-04
Question
We have tried in the past anti-alpha 1 Fetoprotein/AFP antibody for IHC on heart last year. I am using mouse, and We intend to use the antibody for WB next. I am interested in examining heart as well as embryo in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
A. Parker
Verified customer
Asked: 2014-06-12
Answer
I viewed the website and datasheets of our anti-alpha 1 Fetoprotein/AFP antibody and I see that PB9090 has been tested on mouse in both IHC and WB. Thus PB9090 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-06-12
Question
See attached the WB image, lot number and protocol we used for embryo using anti-alpha 1 Fetoprotein/AFP antibody PB9090. Please let me know if you require anything else.
V. Krishna
Verified customer
Asked: 2014-04-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-04-08