Click image to see more details
-
-
-
-
-
+1
Product Info Summary
| SKU: | A15886 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-AKAP 14 Antibody
SKU/Catalog Number
A15886
Size
100μl
Form
Liquid
Description
Boster Bio Anti-AKAP 14 Antibody catalog # A15886. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AKAP 14 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15886)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human AKAP14. AA range:1-50
Reactive Species
A15886 is reactive to AKAP14 in Human, Rat
Calculated molecular weight
22.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A15886 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Validation Images & Assay Conditions
Click image to see more details
Western Blot analysis of Jurkat cells using AKAP 14 Polyclonal Antibody
Click image to see more details
Western blot analysis of lysates from Jurkat, K562, and HUVEC cells, using AKAP14 Antibody. The lane on the right is blocked with the synthesized peptide.
Click image to see more details
Western blot analysis of the lysates from RAW264.7cells using AKAP14 antibody.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4° overnight.3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Click image to see more details
Immunofluorescence analysis of HeLa cells, using AKAP14 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-AKAP 14 Antibody (A15886)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-AKAP 14 Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-AKAP 14 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
5 Customer Q&As for Anti-AKAP 14 Antibody
Question
I was wanting to use your anti-AKAP 14 antibody for WB for human bronchial epithelial cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human bronchial epithelial cell identification?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
You can see on the product datasheet, A15886 anti-AKAP 14 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human bronchial epithelial cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-25
Question
Here is the WB image, lot number and protocol we used for bronchial epithelial cell using anti-AKAP 14 antibody A15886. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-12-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-27
Question
Is this A15886 anti-AKAP 14 antibody reactive to the isotypes of AKAP14?
Verified Customer
Verified customer
Asked: 2018-12-17
Answer
The immunogen of A15886 anti-AKAP 14 antibody is Synthesized peptide derived from the N-terminal region of human AKAP 14. at AA rangle: 10-90. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-12-17
Question
Is there a BSA free version of anti-AKAP 14 antibody A15886 available?
B. Parker
Verified customer
Asked: 2013-03-25
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-AKAP 14 antibody A15886 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-03-25
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for bronchial epithelial cell using anti-AKAP 14 antibody A15886. Let me know if you need anything else.
K. Jones
Verified customer
Asked: 2013-01-22
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-01-22
