Human GPNMB/Osteoactivin ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human GPNMB/Osteoactivin. 96wells/kit, with removable strips.
Human GPNMB/Osteoactivin ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Sample Volume:||100μl per well|
|Product Name||Human GPNMB/Osteoactivin ELISA Kit PicoKine™
See all Osteoactivin/GPNMB primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Human Osteoactivin/GPNMB with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Osteoactivin/GPNMB tissue specificity: Up-regulated in various cancer cells, including in glioblastoma multiforme. Expressed in many melanoma cells, as well as in tissue macrophages, including liver Kuppfer cells and lung alveolar macrophages, in podocytes and in some cells of the ciliary body of the eye (at protein level). Hardly detectable in healthy brain. .|
|Cite This Product||Human GPNMB/Osteoactivin ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1251)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Immunogen/Standard||Expression system for standard: NSO; Immunogen sequence: A22-P486|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Gene/Protein Basic Information For GPNMB (Source: Uniprot.org, NCBI)
|NCBI Gene Id||10457|
|Species Of This Entry||Human|
|Protein Name||Transmembrane glycoprotein NMB|
|Alternative Names||Osteoactivin/GPNMB|DC-HIL; glycoprotein (transmembrane) nmb; glycoprotein NMB; GPNMB; HGFIN; HGFINglycoprotein nmb-like protein; NMBosteoactivin; Osteoactivin; Transmembrane glycoprotein HGFIN; transmembrane glycoprotein NMB|
|Post Tranlational Modifications||Glycosylation; Phosphorylation; Methylation; Biotinylation; Cleavage; Amidation|
|Subcellular Localization||Cell membrane; Single-pass type I membrane protein. Melanosome membrane; Single-pass type I membrane protein. Early endosome membrane; Single-pass type I membrane protein. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.|
|Gene Location||7p15.3, on Chromasome 7, gene sequence: NC_000007.14 (23246766..23275110)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For GPNMB (Source: Uniprot.org, NCBI)
|Protein Function||Could be a melanogenic enzyme. .|
|Tissue Specificity||Up-regulated in various cancer cells, including in glioblastoma multiforme. Expressed in many melanoma cells, as well as in tissue macrophages, including liver Kuppfer cells and lung alveolar macrophages, in podocytes and in some cells of the ciliary body of the eye (at protein level). Hardly detectable in healthy brain.|
|Related Diseases||Neoplasms; Malignant Neoplasms; Melanoma; Neoplasm Metastasis; Glaucoma; Intraocular Pressure Disorder; Inflammation; Mammary Neoplasms; Carcinoma; Malignant Neoplasm Of Breast; Neoplasm Invasiveness; Malignant Paraganglionic Neoplasm; Iris Diseases; Nervousness; Kidney Diseases; Fibrosis; Cell Transformation, Neoplastic; Cell Invasion; Glioma; Injury To Kidney|
|Related Pathways||Pathogenesis; Osteoblast Differentiation; Localization; Cell Differentiation; Regeneration; Bone Resorption; Cell Adhesion; Cell Proliferation; Glycosylation; Transport; Phagocytosis; Cell Cycle; Cell Growth; Angiogenesis; Cell Activation; Cell Migration; Osteoblast Development; Lung Development; Osteoclast Differentiation; Immune Response|
|Background||Transmembrane glycoprotein NMB is a protein that in humans is encoded by the GPNMB gene. In osteoblast progenitor cells, GPNMB works as a positive regulator of osteoblast differentiation during later stages of matrix maturation and mineralization that is mediated at least in part by BMP-2 in a SMAD1 dependent manner to promote osteoblast differentiation. GPNMB can enhance the repairing process in bone fracture, demonstrated by its high expression during chondrogenesis(soft callus) and osteogenesis(hard callus) compared to the intact femurs that is why Osteoactivin(OA) could be a novel therapeutic agent used to treat generalized osteoporosis or localized osteopenia during fracture repair by stimulating bone growth and regeneration. Similarly, GPNMB expression increases during osteoclast differentiation and it is functionally implicated in this process, possibly by promoting the fusion of osteoclast progenitor cells.|
|Scientific References||PMID: 7814155 by Weterman M.A.J., et al. NMB, a novel gene, is expressed in low-metastatic human melanoma cell lines and xenografts.|
PMID: 12609765 by Bandari P.S., et al. Hematopoietic growth factor inducible neurokinin-1 type: a transmembrane protein that is similar to neurokinin 1 interacts with substance P.
Kit Components And Assay QC Data Details Of Human GPNMB/Osteoactivin ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK1251-CAP||96-well plate precoated with anti-Human Osteoactivin/GPNMB antibody||1|
|EK1251-ST||lyophilized recombinant Human Osteoactivin/GPNMB standard||10ng/tube|
|EK1251-DA||biotinylated anti-Human Osteoactivin/GPNMB antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK1251 Human GPNMB/Osteoactivin ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Human GPNMB/Osteoactivin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Human GPNMB/Osteoactivin ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
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