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- Table of Contents
Lep antibody (PB9658) performs well in ELISA, WB and shows reactivity with Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 4 Publications
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Western blot analysis of Leptin using anti-Leptin antibody (PB9658).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: NIH3T3 Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Leptin antigen affinity purified polyclonal antibody (Catalog # PB9658) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Leptin at approximately 16 kDa. The expected band size for Leptin is at 16 kDa.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: NIH3T3 Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Leptin antigen affinity purified polyclonal antibody (Catalog # PB9658) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Leptin at approximately 16 kDa. The expected band size for Leptin is at 16 kDa.
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Anti-Leptin Antibody Picoband®
Lep antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse | Rabbit | ELISA, WB |
Tested Applications
Lep antibody was positively detected by WB in:
NIH3T3 Whole Cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
ELISA
0.1-0.5μg/ml
-
Western blot
0.1-0.5μg/ml
Mouse
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Leptin Antibody Picoband®
View all LEP Antibodies
View all LEP Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of mouse Leptin, different from the related human sequence by five amino acids, and from the related rat sequence by two amino acids.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
16 kDa
Calculated Molecular Weight
18.7 kDa
Background of Lep
Leptin is a protein product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of Leptin have been found to be obese and diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding Leptin have been isolated from human, simian, mouse, and rat cells. The expression of Leptin mRNA has been shown to be restricted to adipose tissue. Although regulation of fat stores is deemed to be the primary function of leptin, it also plays a role in other physiological processes, as evidenced by its multiple sites of synthesis other than fat cells, and the multiple cell types beside hypothalamic cells that have leptin receptors. Many of these additional functions are yet to be defined.
Cite This Product
Anti-Leptin Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9658)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
94%
High Homology
Human
86%
Moderate Homology
Cow
86%
Moderate Homology
Rabbit
83%
Moderate Homology
Sheep
83%
Moderate Homology
Dog
78%
Moderate Homology
Monkey
78%
Moderate Homology
Zebrafish
33%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Lep antibody
Publications
Application:
lncRNA Ftx promotes aerobic glycolysis and tumor progression through the PPAR? pathway in hepatocellular carcinoma
PMID: 29845188
Cheng Ys, Dai Dz, Dai Y. Reprod Toxicol. 2010 Jul;29(4):421-6. Doi: 10.1016/J.Reprotox.2010.03.001. Epub 2010 Mar 20. Testis Dysfunction By Isoproterenol Is Mediated By Upregulating Endothelin Receptor A, Leptin And Protein Kinase Cvarepsilon And ...
PMID: 20307649
Yuan Y, Zhang J, Cai L, Ding C, Wang X, Chen H, Wang X, Yan J, Lu J. Oncol Rep. 2013 Jun;29(6):2291-6. Doi: 10.3892/Or.2013.2390. Epub 2013 Apr 8. Leptin Induces Cell Proliferation And Reduces Cell Apoptosis By Activating C-Myc In Cervical Cancer.
PMID: 23588620
Qin Jh, Ma Jz, Yang Xw, Hu Yj, Zhou J, Fu Lc, Tian Rh, Liu S, Xu G, Shen Xl. Nat Prod Bioprospect. 2015 Jun;5(3):159-66. Doi: 10.1007/S13659-015-0063-5. Epub 2015 Jun 16. A Triterpenoid Inhibited Hormone-Induced Adipocyte Differentiation And Allev...
PMID: 26077651
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Product Promise
We promise Anti-Leptin Antibody Picoband® performs excellently in relevant applications for ELISA, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
PB9658
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 18, 2026