Click image to see more details
Product Info Summary
| SKU: | PA1671 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Retinal dehydrogenase 1 ALDH1A1 Antibody Picoband®
SKU/Catalog Number
PA1671
BA3672 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Retinal dehydrogenase 1 ALDH1A1 Antibody catalog # PA1671. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Retinal dehydrogenase 1 ALDH1A1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1671)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human ALDH1A1, different from the related rat and mouse sequences by one amino acid.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1671 is reactive to ALDH1A1 in Human, Mouse, Rat
Observed Molecular Weight
55 kDa
Calculated molecular weight
54.9 kDa
Background of ALDH1A1
ALDH1A1 (Aldehyde dehydrogenase 1 family, member A1), also called ALDH1, RALDH1 or ALDH, LIVER CYTOSOLIC, is an enzyme that in humans is encoded by the ALDH1A1 gene. And this protein belongs to the aldehyde dehydrogenases family of proteins. The ALDH1A1 gene is mapped on 9q21.13. ALDH1A1 also belongs to the group of corneal crystallins that help maintain the transparency of the cornea.ALDH1A1 is associated with a low Km for NAD, a high Km for acetaldehyde, and is strongly inactivated by disulfiram. The ALDH1 gene is about 53 kb long and is divided into 13 exons. Retinaldehyde is generated by ADH1 from retinol, and its concentration is determined in large part by its subsequent catabolism by RALDH1 to retinoic acid.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1671 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5 μg/ml, Human, Mouse, Rat
,
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: human A549 whole cell lysates, human HepG2 whole cell lysates, rat liver tissue lysates, rat kidney tissue lysates, mouse liver tissue lysates, mouse kidney tissue lysates
IHC: human lung cancer
ICC/IF: A549 cell
FCM: HepG2 cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of ALDH1A1 using anti-ALDH1A1 antibody (PA1671).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: rat liver tissue lysates,
Lane 4: rat kidney tissue lysates,
Lane 5: mouse liver tissue lysates,b
Lane 6: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDH1A1 antigen affinity purified polyclonal antibody (Catalog # PA1671) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALDH1A1 at approximately 55 kDa. The expected band size for ALDH1A1 is at 55 kDa.
Click image to see more details
IHC analysis of ALDH1A1 using anti-ALDH1A1 antibody (PA1671).
ALDH1A1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ALDH1A1 Antibody (PA1671) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IF analysis of ALDH1A1 using anti-ALDH1A1 antibody (PA1671).
ALDH1A1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ALDH1A1 Antibody (PA1671) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Flow Cytometry analysis of HepG2 cells using anti-ALDH1A1 antibody (PA1671).
Overlay histogram showing HepG2 cells stained with PA1671 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ALDH1A1 Antibody (PA1671, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-Retinal dehydrogenase 1 ALDH1A1 Antibody Picoband® (PA1671)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Retinal dehydrogenase 1 ALDH1A1 Antibody Picoband®?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Retinal dehydrogenase 1 ALDH1A1 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
17 Customer Q&As for Anti-Retinal dehydrogenase 1 ALDH1A1 Antibody Picoband®
Question
Our lab used your anti-ALDH1A1 antibody for WB on nasal cavity epithelium in a previous project. I am using human, and I plan to use the antibody for IHC next. I am looking for examining nasal cavity epithelium as well as liver in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
I took a look at the website and datasheets of our anti-ALDH1A1 antibody and it seems that PA1671 has been tested on human in both WB and IHC. Thus PA1671 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-03-13
Question
Will PA1671 anti-ALDH1A1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
M. Brown
Verified customer
Asked: 2020-02-19
Answer
As indicated on the product datasheet, PA1671 anti-ALDH1A1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-19
Question
We are currently using anti-ALDH1A1 antibody PA1671 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-17
Answer
The anti-ALDH1A1 antibody (PA1671) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-17
Question
I am interested in using your anti-ALDH1A1 antibody for ethanol oxidation studies. Has this antibody been tested with western blotting on colo320 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
Thank you for your inquiry. This PA1671 anti-ALDH1A1 antibody is tested on rat lung tissue, tissue lysate, colo320 cell lysate, cancer tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-11-13
Question
Do you have a BSA free version of anti-ALDH1A1 antibody PA1671 available?
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ALDH1A1 antibody PA1671 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-15
Question
I was wanting to use your anti-ALDH1A1 antibody for WB for rat lens on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat lens identification?
N. Li
Verified customer
Asked: 2019-06-06
Answer
As indicated on the product datasheet, PA1671 anti-ALDH1A1 antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat lens in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-06
Question
Is this PA1671 anti-ALDH1A1 antibody reactive to the isotypes of ALDH1A1?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
The immunogen of PA1671 anti-ALDH1A1 antibody is A synthetic peptide corresponding to a sequence in the middle region of human ALDH1A1(283-303aa DLDNAVEFAHHGVFYHQGQCC), different from the related rat and mouse sequences by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-31
Question
I see that the anti-ALDH1A1 antibody PA1671 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-05-29
Question
My question regarding product PA1671, anti-ALDH1A1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-03-15
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1671 anti-ALDH1A1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-03-15
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lens using anti-ALDH1A1 antibody PA1671. Let me know if you need anything else.
Z. Rodriguez
Verified customer
Asked: 2018-04-17
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-04-17
Question
Does anti-ALDH1A1 antibody PA1671 work on primate WB with lens?
Verified Customer
Verified customer
Asked: 2018-02-28
Answer
Our lab technicians have not validated anti-ALDH1A1 antibody PA1671 on primate. You can run a BLAST between primate and the immunogen sequence of anti-ALDH1A1 antibody PA1671 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate lens in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-02-28
Question
I am looking for to test anti-ALDH1A1 antibody PA1671 on rat lens for research purposes, then I may be interested in using anti-ALDH1A1 antibody PA1671 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-01-03
Answer
The products we sell, including anti-ALDH1A1 antibody PA1671, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-01-03
Question
Does anti-ALDH1A1 antibody PA1671 work for WB with lens?
Verified Customer
Verified customer
Asked: 2017-09-18
Answer
According to the expression profile of lens, ALDH1A1 is highly expressed in lens. So, it is likely that anti-ALDH1A1 antibody PA1671 will work for WB with lens.
Boster Scientific Support
Answered: 2017-09-18
Question
We were happy with the WB result of your anti-ALDH1A1 antibody. However we have been able to see positive staining in lens cytosol using this antibody. Is that expected? Could you tell me where is ALDH1A1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-05-12
Answer
From what I have seen in literature, lens does express ALDH1A1. Generally ALDH1A1 expresses in cytoplasm, cytosol. Regarding which tissues have ALDH1A1 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Erythrocyte, Pubmed ID: 2776714, 19608861
Lens, Pubmed ID: 19296407
Liver, Pubmed ID: 2987944, 6723659, 8214422, 24275569
Boster Scientific Support
Answered: 2017-05-12
Question
We have seen staining in mouse colon. What should we do? Is anti-ALDH1A1 antibody supposed to stain colon positively?
E. Baker
Verified customer
Asked: 2016-03-16
Answer
Based on literature colon does express ALDH1A1. Based on Uniprot.org, ALDH1A1 is expressed in nasal cavity epithelium, liver, lens, colon, erythrocyte, among other tissues. Regarding which tissues have ALDH1A1 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Erythrocyte, Pubmed ID: 2776714, 19608861
Lens, Pubmed ID: 19296407
Liver, Pubmed ID: 2987944, 6723659, 8214422, 24275569
Boster Scientific Support
Answered: 2016-03-16
Question
Is a blocking peptide available for product anti-ALDH1A1 antibody (PA1671)?
H. Brown
Verified customer
Asked: 2013-12-24
Answer
We do provide the blocking peptide for product anti-ALDH1A1 antibody (PA1671). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2013-12-24
Question
I have attached the WB image, lot number and protocol we used for lens using anti-ALDH1A1 antibody PA1671. Please let me know if you require anything else.
S. Li
Verified customer
Asked: 2013-06-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-06-19
