Anti-Retinal dehydrogenase 1 ALDH1A1 Antibody

Aldehyde Dehydrogenase 1-A1/ALDH1A1 antibody

Boster Bio Anti-Retinal dehydrogenase 1 ALDH1A1 Antibody catalog # PA1671. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: PA1671
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-Retinal dehydrogenase 1 ALDH1A1 Antibody

View all Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibodies

SKU/Catalog Number

PA1671

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Retinal dehydrogenase 1 ALDH1A1 Antibody catalog # PA1671. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Retinal dehydrogenase 1 ALDH1A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1671)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human ALDH1A1, different from the related rat and mouse sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1671 is reactive to ALDH1A1 in Human, Mouse, Rat

Applications

PA1671 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

54.862kDa

Background of Aldehyde Dehydrogenase 1-A1/ALDH1A1

ALDH1A1 (Aldehyde dehydrogenase 1 family, member A1), also called ALDH1, RALDH1 or ALDH, LIVER CYTOSOLIC, is an enzyme that in humans is encoded by the ALDH1A1 gene. And this protein belongs to the aldehyde dehydrogenases family of proteins. The ALDH1A1 gene is mapped on 9q21.13. ALDH1A1 also belongs to the group of corneal crystallins that help maintain the transparency of the cornea.ALDH1A1 is associated with a low Km for NAD, a high Km for acetaldehyde, and is strongly inactivated by disulfiram. The ALDH1 gene is about 53 kb long and is divided into 13 exons. Retinaldehyde is generated by ADH1 from retinol, and its concentration is determined in large part by its subsequent catabolism by RALDH1 to retinoic acid.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For ALDH1A1 (Source: Uniprot.org, NCBI)

Gene Name

ALDH1A1

Full Name

Retinal dehydrogenase 1

Weight

54.862kDa

Superfamily

aldehyde dehydrogenase family

Alternative Names

acetaldehyde dehydrogenase 1; ALDC; aldehyde dehydrogenase 1 family, member A1; aldehyde dehydrogenase 1, soluble; Aldehyde Dehydrogenase 1A1; Aldehyde Dehydrogenase 1-A1; Aldehyde dehydrogenase family 1 member A1; Aldehyde dehydrogenase, cytosolic; aldehyde dehydrogenase, liver cytosolic; ALDH class 1; ALDH1; ALDH11; ALDH1A1; ALDH-E1; ALHDII; EC 1.2.1; EC 1.2.1.36; MGC2318; PUMB1; RALDH 1; RALDH1; retinal dehydrogenase 1; retinaldehyde dehydrogenase 1 ALDH1A1 ALDC, ALDH-E1, ALDH1, ALDH11, HEL-9, HEL-S-53e, HEL12, PUMB1, RALDH1 aldehyde dehydrogenase 1 family member A1 retinal dehydrogenase 1|ALDH class 1|ALHDII|RALDH 1|acetaldehyde dehydrogenase 1|aldehyde dehydrogenase 1, soluble|aldehyde dehydrogenase, liver cytosolic|epididymis luminal protein 12|epididymis luminal protein 9|epididymis secretory sperm binding protein Li 53e|retinaldehyde dehydrogenase 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ALDH1A1, check out the ALDH1A1 Infographic

ALDH1A1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ALDH1A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA1671 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Li Y,Chen F,Chen J,Chan S,He Y,Liu W,Zhang G.Disulfiram/Copper Induces Antitumor Activity against Both Nasopharyngeal Cancer Cells and Cancer-Associated Fibroblasts through ROS/MAPK and Ferroptosis Pathways.Cancers (Basel).2020 Jan 6;12(1):138.doi:10.3390
Species: Human,Mouse
PA1671 usage in article: APP:WB, SAMPLE:NPC CELL LINES AND NP69 CELL, DILUTION:1:100

Breast cancer stem cells expressing different stem cell markers exhibit distinct biological characteristics

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17 Customer Q&As for Anti-Retinal dehydrogenase 1 ALDH1A1 Antibody

Question

Our lab used your anti-ALDH1A1 antibody for WB on nasal cavity epithelium in a previous project. I am using human, and I plan to use the antibody for IHC next. I am looking for examining nasal cavity epithelium as well as liver in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2020-03-13

Answer

I took a look at the website and datasheets of our anti-ALDH1A1 antibody and it seems that PA1671 has been tested on human in both WB and IHC. Thus PA1671 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-03-13

Question

Will PA1671 anti-ALDH1A1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

M. Brown

Verified customer

Asked: 2020-02-19

Answer

As indicated on the product datasheet, PA1671 anti-ALDH1A1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-02-19

Question

We are currently using anti-ALDH1A1 antibody PA1671 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2019-12-17

Answer

The anti-ALDH1A1 antibody (PA1671) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-12-17

Question

I am interested in using your anti-ALDH1A1 antibody for ethanol oxidation studies. Has this antibody been tested with western blotting on colo320 cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-11-13

Answer

Thank you for your inquiry. This PA1671 anti-ALDH1A1 antibody is tested on rat lung tissue, tissue lysate, colo320 cell lysate, cancer tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-11-13

Question

Do you have a BSA free version of anti-ALDH1A1 antibody PA1671 available?

Verified Customer

Verified customer

Asked: 2019-10-15

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ALDH1A1 antibody PA1671 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-10-15

Question

I was wanting to use your anti-ALDH1A1 antibody for WB for rat lens on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat lens identification?

N. Li

Verified customer

Asked: 2019-06-06

Answer

As indicated on the product datasheet, PA1671 anti-ALDH1A1 antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat lens in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-06-06

Question

Is this PA1671 anti-ALDH1A1 antibody reactive to the isotypes of ALDH1A1?

Verified Customer

Verified customer

Asked: 2019-05-31

Answer

The immunogen of PA1671 anti-ALDH1A1 antibody is A synthetic peptide corresponding to a sequence in the middle region of human ALDH1A1(283-303aa DLDNAVEFAHHGVFYHQGQCC), different from the related rat and mouse sequences by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-05-31

Question

I see that the anti-ALDH1A1 antibody PA1671 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-05-29

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-05-29

Question

My question regarding product PA1671, anti-ALDH1A1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-03-15

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1671 anti-ALDH1A1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-03-15

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lens using anti-ALDH1A1 antibody PA1671. Let me know if you need anything else.

Z. Rodriguez

Verified customer

Asked: 2018-04-17

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-04-17

Question

Does anti-ALDH1A1 antibody PA1671 work on primate WB with lens?

Verified Customer

Verified customer

Asked: 2018-02-28

Answer

Our lab technicians have not validated anti-ALDH1A1 antibody PA1671 on primate. You can run a BLAST between primate and the immunogen sequence of anti-ALDH1A1 antibody PA1671 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate lens in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-02-28

Question

I am looking for to test anti-ALDH1A1 antibody PA1671 on rat lens for research purposes, then I may be interested in using anti-ALDH1A1 antibody PA1671 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-01-03

Answer

The products we sell, including anti-ALDH1A1 antibody PA1671, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-01-03

Question

Does anti-ALDH1A1 antibody PA1671 work for WB with lens?

Verified Customer

Verified customer

Asked: 2017-09-18

Answer

According to the expression profile of lens, ALDH1A1 is highly expressed in lens. So, it is likely that anti-ALDH1A1 antibody PA1671 will work for WB with lens.

Boster Scientific Support

Answered: 2017-09-18

Question

We were happy with the WB result of your anti-ALDH1A1 antibody. However we have been able to see positive staining in lens cytosol using this antibody. Is that expected? Could you tell me where is ALDH1A1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2017-05-12

Answer

From what I have seen in literature, lens does express ALDH1A1. Generally ALDH1A1 expresses in cytoplasm, cytosol. Regarding which tissues have ALDH1A1 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Erythrocyte, Pubmed ID: 2776714, 19608861
Lens, Pubmed ID: 19296407
Liver, Pubmed ID: 2987944, 6723659, 8214422, 24275569

Boster Scientific Support

Answered: 2017-05-12

Question

We have seen staining in mouse colon. What should we do? Is anti-ALDH1A1 antibody supposed to stain colon positively?

E. Baker

Verified customer

Asked: 2016-03-16

Answer

Based on literature colon does express ALDH1A1. Based on Uniprot.org, ALDH1A1 is expressed in nasal cavity epithelium, liver, lens, colon, erythrocyte, among other tissues. Regarding which tissues have ALDH1A1 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Erythrocyte, Pubmed ID: 2776714, 19608861
Lens, Pubmed ID: 19296407
Liver, Pubmed ID: 2987944, 6723659, 8214422, 24275569

Boster Scientific Support

Answered: 2016-03-16

Question

Is a blocking peptide available for product anti-ALDH1A1 antibody (PA1671)?

H. Brown

Verified customer

Asked: 2013-12-24

Answer

We do provide the blocking peptide for product anti-ALDH1A1 antibody (PA1671). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2013-12-24

Question

I have attached the WB image, lot number and protocol we used for lens using anti-ALDH1A1 antibody PA1671. Please let me know if you require anything else.

S. Li

Verified customer

Asked: 2013-06-19

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-06-19

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Total: $370

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