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- Table of Contents
ALDH1B1 antibody (PB10037) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Monkey, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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Flow Cytometry analysis of HEL cells using anti-ALDH1B1 antibody (PB10037).
Overlay histogram showing HEL cells stained with PB10037 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ALDH1B1 Antibody (PB10037, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing HEL cells stained with PB10037 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ALDH1B1 Antibody (PB10037, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-ALDH1B1 Antibody Picoband®
ALDH1B1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Monkey, Mouse, Rat | Rabbit | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
ALDH1B1 antibody was positively detected by WB in:
human HepG2 whole cell, human K562 whole cell, human A431 whole cell, human A549 whole cell, human U20S whole cell, human Hela whole cell, monkey COS-7 whole cell, rat liver tissue, rat testis tissue, rat RH35 whole cell, mouse brain tissue, mouse liver tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ALDH1B1 antibody was positively detected by IHC in:
human colon cancer tissue, human colonic adenocarcinoma tissue, human endometrial adenocarcinoma tissue, human hepatocellular carcinoma tissue, human laryngeal squamous cell carcinoma tissue, mouse colon tissue, rat colon tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
ALDH1B1 antibody was positively detected by ICC/IF in:
A431 cell
ALDH1B1 antibody was positively detected by FC in:
HEL cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Monkey, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence
5μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Monkey
Tested
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Dog
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-ALDH1B1 Antibody Picoband®
View all ALDH1B1 Antibodies
View all ALDH1B1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human ALDH1B1, different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
55 kDa
Calculated Molecular Weight
57.2 kDa
Background of ALDH1B1
Aldehyde dehydrogenase X, mitochondrial is an enzyme that in humans is encoded by the ALDH1B1 gene. This protein belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. This gene does not contain introns in the coding sequence. The variation of this locus may affect the development of alcohol-related problems.
Cite This Product
Anti-ALDH1B1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10037)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Dog
100%
High Homology
Monkey
100%
High Homology
Mouse
98%
High Homology
Rat
95%
High Homology
Cow
93%
High Homology
Drosophila
71%
Moderate Homology
Rabbit
68%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for ALDH1B1 antibody
Immunohistochemistry (IHC) protocol for ALDH1B1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for ALDH1B1 antibody
Flow Cytometry (FC) protocol for ALDH1B1 antibody
Publications
Application:
Species:
Spatial multiomics atlas reveals smooth muscle phenotypic transformation and metabolic reprogramming in diabetic macroangiopathy
Published: 2024-10-12
Journal: Cardiovascular Diabetology
Application: IF
Impact Factor: 8.50
Species: Human
PMID: 39395983
Lysine β-hydroxybutyrylation promotes lipid accumulation in alcoholic liver disease
Published: 2023-11-25
Journal: BIOCHEMICAL PHARMACOLOGY
Application: WB
Impact Factor: 5.80
Species: Human
PMID: 38012969
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Product Promise
We promise Anti-ALDH1B1 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
PB10037
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 11, 2026