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Product Info Summary
| SKU: | A03893 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Aldolase B ALDOB Antibody
SKU/Catalog Number
A03893
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Aldolase B ALDOB Antibody catalog # A03893. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Aldolase B ALDOB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03893)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human ALDOB. AA range:111-160
Reactive Species
A03893 is reactive to ALDOB in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
39.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03893 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of HuvEc cells using Aldolase B Polyclonal Antibody
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Western blot analysis of lysates from MCF-7 and HUVEC cells, using ALDOB Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from 293 cells using ALDOB antibody.
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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4° overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Specific Publications For Anti-Aldolase B ALDOB Antibody (A03893)
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Customer Q&As
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4 Customer Q&As for Anti-Aldolase B ALDOB Antibody
Question
Does anti-Aldolase B antibody A03893 work for WB with liver?
E. Zhao
Verified customer
Asked: 2019-05-01
Answer
According to the expression profile of liver, ALDOB is highly expressed in liver. So, it is likely that anti-Aldolase B antibody A03893 will work for WB with liver.
Boster Scientific Support
Answered: 2019-05-01
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Aldolase B antibody A03893. Let me know if you need anything else.
A. Jha
Verified customer
Asked: 2018-02-02
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-02-02
Question
We need to test anti-Aldolase B antibody A03893 on rat liver for research purposes, then I may be interested in using anti-Aldolase B antibody A03893 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
S. Bhatt
Verified customer
Asked: 2015-10-16
Answer
The products we sell, including anti-Aldolase B antibody A03893, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-10-16
Question
Does anti-Aldolase B antibody A03893 work on feline WB with jejunal mucosa?
A. Edwards
Verified customer
Asked: 2014-10-24
Answer
Our lab technicians have not validated anti-Aldolase B antibody A03893 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Aldolase B antibody A03893 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline jejunal mucosa in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-10-24
