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Product Info Summary
| SKU: | M00522-3 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Dog, Human, Monkey |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC |
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Product info
Product Name
Anti-alpha 1 Fetoprotein (AFP) Mouse Monoclonal Antibody [Clone ID: OTI2A9]
SKU/Catalog Number
M00522-3
Size
100 µl
Description
Boster Bio AFP (alpha-fetoprotein) mouse monoclonal antibody, clone OTI2A9 (formerly 2A9). Catalog# M00522-3. Tested in Flow Cytometry, IF, IHC. This antibody reacts with Human, Monkey, Dog.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-alpha 1 Fetoprotein (AFP) Mouse Monoclonal Antibody [Clone ID: OTI2A9] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00522-3)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI2A9
Isotype
IgG2b
Immunogen
Full length human recombinant protein of human AFP (NP_001125) produced in HEK293T cell.
Reactive Species
M00522-3 is reactive to AFP in Dog, Human, Monkey
Calculated molecular weight
68.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00522-3 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:150, IF 1:100, FLOW 1:100
Validation Images & Assay Conditions
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-AFP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells transfected with either AFP (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-AFP antibody (M00522-3)
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Flow cytometric Analysis of Jurkat cells
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Anti-AFP mouse monoclonal antibody (M00522-3) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AFP.
Specific Publications For Anti-alpha 1 Fetoprotein (AFP) Mouse Monoclonal Antibody [Clone ID: OTI2A9] (M00522-3)
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