This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
FCGR3A antibody (A01408-1) performs well in ELISA, Flow Cytometry, IF, IHC, ICC and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
Filter:
Flow Cytometry analysis of human PBMC cells using anti-CD16 antibody (A01408-1).
Overlay histogram showing human PBMC cells stained with A01408-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD16 Antibody (A01408-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Overlay histogram showing human PBMC cells stained with A01408-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD16 Antibody (A01408-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Bundle Save
Anti-CD16/FCGR3A Antibody
FCGR3A antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, Flow Cytometry, IF, IHC, ICC |
Tested Applications
FCGR3A antibody was positively detected by IHC in:
human liver cancer tissue, human rectal cancer tissue, human rectal cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
FCGR3A antibody was positively detected by ICC/IF in:
K562 cell
FCGR3A antibody was positively detected by FC in:
HL-60 cell
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Immunocytochemistry/Immunofluorescence
5μg/ml
Flow Cytometry (Fixed)
1-3μg/1x106 cells
ELISA
0.1-0.5μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
Filter:
Boster Guarantee:What is this?
IHC
IF
ELISA
Human
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-CD16/FCGR3A Antibody
View all FCGR3A Antibodies
View all FCGR3A Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human CD16 recombinant protein (Position: Q101-D166).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Calculated Molecular Weight
29.1 kDa
Background of FCGR3A
Low affinity immunoglobulin gamma Fc region receptor III-A is a protein that in humans is encoded by the FCGR3A gene. This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other other antibody-dependent responses. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Cite This Product
Anti-CD16/FCGR3A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01408-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
94%
High Homology
Mouse
71%
Moderate Homology
Rabbit
71%
Moderate Homology
Rat
65%
Low homology
Sheep
61%
Low homology
Dog
59%
Low homology
Cow
56%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Immunohistochemistry (IHC) protocol for FCGR3A antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for FCGR3A antibody
Flow Cytometry (FC) protocol for FCGR3A antibody
Publications
Application:
Species:
IGF2BP3 overexpression predicts poor prognosis and correlates with immune infiltration in bladder cancer
Published: 2023-02-03
Journal: BMC CANCER
Application: IHC
Impact Factor: 3.80
Species: Human
PMID: 36732736
Expression of Programmed Death-Ligand 1 in Laryngeal Carcinoma and its Effects on Immune Cell Subgroup Infiltration
Published: 2018-10-25
Journal: PATHOLOGY & ONCOLOGY RESEARCH
Application: IHC
Impact Factor: 1.94
Species: Human
PMID: 30361911
Review
Have you used Anti-CD16/FCGR3A Antibody?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-CD16/FCGR3A Antibody performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, ICC. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A01408-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 7, 2026