Anti-Alpha-1-Trypsin SERPINA1 Antibody Peroxidase Conjugated

Product Name

Anti-Alpha-1-Trypsin SERPINA1 Antibody Peroxidase Conjugated

View all SERPINA1 Antibodies

SKU/Catalog Number

H00720

Size

100ug

Form

Lyophilized

Description

Boster Bio Anti-Alpha-1-Trypsin SERPINA1 Antibody Peroxidase Conjugated (Catalog # H00720). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship at ambient temperature.)

Cite This Product

Anti-Alpha-1-Trypsin SERPINA1 Antibody Peroxidase Conjugated (Boster Biological Technology, Pleasanton CA, USA, Catalog # H00720)

Host

Goat

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, antibody formulated with stabilizing protein (10 mg/mL) - Immunoglobulin and Protease free, 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Anti-alpha 1 Anti-Trypsin Antibody was produced by repeated immunizations with native α1-Anti-Trypsin protein from human plasma.

Reactive Species

H00720 is reactive to SERPINA1 in Human

Calculated molecular weight

46.7 kDa

Background of SERPINA1

Anti-Alpha 1-Anti-Trypsin Antibody detects a1-Anti-Trypsin protein. a1-Anti-Trypsin is an inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Anti-Trypsin irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form of Antitrypsin inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Defects in SERPINA1 are the cause emphysema, liver cirrhosis and liver failure. Environmental factors, particularly cigarette smoking, greatly increase the risk of emphysema at an earlier age. Anti-Alpha-1-Anti-Trypsin Antibody is ideal for investigators involved in Cell Signaling, Signal Transduction and Cancer research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

H00720 is guaranteed for ELISA, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:1,000 - 1:5,000
IHC: 1:200 - 1:1,000
WB: 1:500 - 1:2,500
Anti-Alpha 1 anti-Trypsin Antibody is tested by ELISA and western blot. This product detects a single band of the expected apparent molecular weight (~47 kDa) and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Validation Images & Assay Conditions

See full target information SERPINA1

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