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Product Info Summary
| SKU: | PB10097 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Monkey, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-alpha 1 Antitrypsin/SERPINA1 Antibody Picoband®
SKU/Catalog Number
PB10097
PB1151 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
SERPINA1 (alpha-1 antitrypsin; AAT) is a secreted serine protease inhibitor with targets including elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator; the page notes defects can cause emphysema or liver disease. Assay context: antibody validated for WB (human/monkey/mouse) with on-page starting dilution guidance (0.1–0.5 µg/mL). Often interpreted with protease-generation markers such as KLK1 and coagulation/fibrinolysis inhibitors like SERPINF2 to contextualize net proteolytic pressure in tissues or biofluids (putative).
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-alpha 1 Antitrypsin/SERPINA1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10097)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human SERPINA1 recombinant protein (Position: E25-T204). Human SERPINA1 shares 72.8% amino acid (aa) sequence identity with rat SERPINA1.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB10097 is reactive to SERPINA1 in Human, Monkey, Mouse
Observed Molecular Weight
53 kDa
Calculated molecular weight
46.7 kDa
Background of SERPINA1
SERPINA1 is also known as PI, A1A or AAT. This gene is mapped to 14q32.1. The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB10097 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Monkey, Mouse
Positive Control
WB: human T47D whole cell, monkey COS-7 whole cell, mouse kidney tissue
Validation Images & Assay Conditions
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Western blot analysis of SERPINA1 using anti-SERPINA1 antibody (PB10097).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human T47D whole cell lysates,
Lane 2: monkey COS-7 whole cell lysates,
Lane 3: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERPINA1 antigen affinity purified polyclonal antibody (PB10097) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SERPINA1 at approximately 53 kDa. The expected band size for SERPINA1 is at 47 kDa.
Specific Publications For Anti-alpha 1 Antitrypsin/SERPINA1 Antibody Picoband® (PB10097)
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