Human Serpin A12 ELISA Kit PicoKine™

Human Serpin A12 ELISA Kit PicoKine™ (96 Tests). Quantitate Human SERPINA12 in cell culture supernatants, serum and plasma (heparin, EDTA) and urine. Sensitivity: 50pg/ml.

Product Info Summary

SKU: EK1874
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA) and urine.

Product Name

Human Serpin A12 ELISA Kit PicoKine™

See all Serpin A12 products

SKU/Catalog Number

EK1874

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Serpin A12 ELISA Kit PicoKine™ (96 Tests). Quantitate Human SERPINA12 in cell culture supernatants, serum and plasma (heparin, EDTA) and urine. Sensitivity: 50pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Serpin A12 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1874)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: L21-K414

Sensitivity

<50 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1874 is reactive to SERPINA12 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA) and urine.

Application Guarantee

EK1874 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1874-CAP Anti-Human SERPINA12 Pre-coated 96-well strip microplate 1
EK1874-ST Human SERPINA12 Standard 2 vials, 10 ng/tube
EK1874-DA Human SERPINA12 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0250.1600.2630.4910.8521.2901.8782.305

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Serpin A12, Dilution ratio of 1:10, concentration in serum is around 10ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Serpin A12 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2121109546523212356154
Standard deviation11.2468.76245.9310.9079.04332.32
CV (%)5.3%6.2%4.5%4.7%6.4%5.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Serpin A12 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 12122152322202197.623.4%
Sample 21109121512351149117750.534.2%
Sample 354656356598759765946317.035.3%
*number of samples for each test n=16.

Gene/Protein Information For SERPINA12 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q8IW75

Gene ID

145264

Gene Name

SERPINA12

Full Name

Serpin A12

Weight

47.175kDa

Superfamily

serpin family

Alternative Names

antitrypsin), member 12; member 12; OL-64; OL-64Visceral adipose-specific serpin; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase; Serpin A12; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin); Vaspin; Visceral adipose tissue-derived serine protease inhibitor SERPINA12 OL-64 serpin family A member 12 serpin A12|serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12|serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12|vaspin|visceral adipose tissue-derived serine protease inhibitor|visceral adipose-specific SERPIN

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on SERPINA12, check out the SERPINA12 Infographic

SERPINA12 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for SERPINA12: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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9 Customer Q&As for Human Serpin A12 ELISA Kit PicoKine™

Question

Q: Can Serpin A12 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2020-12-03

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2020-12-03

Question

Q: which procedure should I follow in order to thaw whole blood sample for Serpin A12 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2020-10-21

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Serpin A12 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2020-10-21

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2020-07-24

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-07-24

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2020-02-02

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-02-02

Question

Q: can I use citrate plasma as samples in Human Serpin A12 Picokine® ELISA Kit (Catalog # EK1874)?

Verified Customer

Verified customer

Asked: 2019-06-17

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of Serpin A12 causing disruption of its protein structure. Serpin A12 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Serpin A12 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2019-06-17

Question

Q: how do I analyze ELISA data? I measured Serpin A12 level in serum.

Verified Customer

Verified customer

Asked: 2018-05-31

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-05-31

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

E. Moore

Verified customer

Asked: 2017-05-13

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2017-05-13

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring Serpin A12 using the EK1874 Human Serpin A12 Picokine® ELISA Kit?

G. Li

Verified customer

Asked: 2017-05-01

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.

Boster Scientific Support

Answered: 2017-05-01

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2014-07-03

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2014-07-03

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