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Product Info Summary
| SKU: | M00720-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-SERPINA1/Alpha 1 Antitrypsin Rabbit Monoclonal Antibody
SKU/Catalog Number
M00720-1
BM4931 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-SERPINA1/Alpha 1 Antitrypsin Rabbit Monoclonal Antibody catalog # M00720-1. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SERPINA1/Alpha 1 Antitrypsin Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00720-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AOEF-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human SERPINA1
Reactive Species
M00720-1 is reactive to SERPINA1 in Human
Observed Molecular Weight
47 kDa
Calculated molecular weight
46.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00720-1 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:30
Positive Control
WB: human fetal kidney
Validation Images & Assay Conditions
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Western blot analysis of SERPINA1 expression in human fetal kidney lysate.
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Western blot analysis of SERPINA1 using anti-SERPINA1 antibody (M00720-1).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human U20S whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERPINA1 antigen affinity purified monoclonal antibody (M00720-1) at 1:500 overnight at 4°C, then washed with TBS-10%%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SERPINA1 at approximately 47 kDa. The expected band size for SERPINA1 is at 47 kDa.
Specific Publications For Anti-SERPINA1/Alpha 1 Antitrypsin Rabbit Monoclonal Antibody (M00720-1)
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1 Customer Q&As for Anti-SERPINA1/Alpha 1 Antitrypsin Rabbit Monoclonal Antibody
Question
Has M00720-1 been tested for cross-reactivity with cynomolgus monkey A1AT?
Verified customer
Asked: 2019-11-14
Answer
No test/s was done with samples from cynomolgus monkey using the Anti-SERPINA1/Alpha 1 Antitrypsin Rabbit Monoclonal Antibody (M00720-1). The immunogen of it is native protein. It is not certain if it would cross react with cynomolgus monkey.
Boster Scientific Support
Answered: 2019-11-15
