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- Table of Contents
NDUFAB1 antibody (M09620) performs well in Flow Cytometry, IP, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of NDUFAB1 using anti-NDUFAB1 antibody (MM09620).
NDUFAB1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NDUFAB1 Antibody (M09620) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
NDUFAB1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NDUFAB1 Antibody (M09620) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-NDUFAB1 Rabbit Monoclonal Antibody
NDUFAB1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IP, IHC, WB |
Tested Applications
NDUFAB1 antibody was positively detected by WB in:
human A431 whole cell, human HepG2 whole cell, human MCF-7 whole cell, human 293T whole cell, human THP-1 whole cell, human PC-3 whole cell, rat RH35 whole cell, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
NDUFAB1 antibody was positively detected by IHC in:
human colorectal adenocarcinoma tissue, human thyroid cancer tissue, human breast cancer tissue, human placenta tissue, human testicular germ cell tumor tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
IP 1:50
FC 1:50
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
IP
FC
Human
Tested
Tested
Recommend
Recommend
Recommend
Rat
Tested
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Product Information
Product Name
Anti-NDUFAB1 Rabbit Monoclonal Antibody
View all NDUFAB1 Antibodies
View all NDUFAB1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
30N94
Isotype
IgG
Immunogen
A synthesized peptide derived from human NDUFAB1
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
14 kDa
Calculated Molecular Weight
17.4 kDa
Cite This Product
Anti-NDUFAB1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M09620)
Protocols
Western Blot protocol for NDUFAB1 antibody
Immunohistochemistry (IHC) protocol for NDUFAB1 antibody
Review
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Product Promise
We promise Anti-NDUFAB1 Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IP, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M09620
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 24, 2026