Product Info Summary
SKU: | A00883-3 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-alpha 2a Adrenergic Receptor/ADRA2A Antibody Picoband™
View all alpha-2A Adrenergic R/ADRA2A Antibodies
SKU/Catalog Number
A00883-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-alpha 2a Adrenergic Receptor/ADRA2A Antibody Picoband™ catalog # A00883-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-alpha 2a Adrenergic Receptor/ADRA2A Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00883-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human alpha 2a Adrenergic Receptor/ADRA2A recombinant protein (Position: M16-V465).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00883-3 is reactive to ADRA2A in Human, Mouse, Rat
Applications
A00883-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
55 kDa
Calculated molecular weight
50.647kDa
Background of alpha-2A Adrenergic R/ADRA2A
The alpha-2A adrenergic receptor, also known as ADRA2A denotes the human gene encoding it. This gene is mapped to 10q25.2. Alpha-2-adrenergic receptors are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. Studies in mouse revealed that both the alpha2A and alpha2C subtypes were required for normal presynaptic control of transmitter release from sympathetic nerves in the heart and from central noradrenergic neurons; the alpha2A subtype inhibited transmitter release at high stimulation frequencies, whereas the alpha2C subtype modulated neurotransmission at lower levels of nerve activity. This gene encodes alpha2A subtype and it contains no introns in either its coding or untranslated sequences. Alpha-2 adrenergic receptors mediate the catecholamine-induced inhibition of adenylate cyclase through the action of G proteins.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Mouse, Rat
Flow Cytometry, 1-3 µg/1x106 cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of alpha 2a Adrenergic Receptor/ADRA2A using anti-alpha 2a Adrenergic Receptor/ADRA2A antibody (A00883-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat pancreas tissue lysates,
Lane 2: rat small intestine tissue lysates,
Lane 3: mouse pancreas tissue lysates,
Lane 4: mouse small intestine tissue lysate.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-alpha 2a Adrenergic Receptor/ADRA2A antigen affinity purified polyclonal antibody (Catalog # A00883-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for alpha 2a Adrenergic Receptor/ADRA2A at approximately 55 kDa. The expected band size for alpha 2a Adrenergic Receptor/ADRA2A is at 49 kDa.
Click image to see more details
Figure 2. Flow Cytometry analysis of HEL cells using anti-alpha 2a Adrenergic Receptor/ADRA2A antibody (A00883-3).
Overlay histogram showing HEL cells stained with A00883-3 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-alpha 2a Adrenergic Receptor/ADRA2A Antibody (A00883-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For ADRA2A (Source: Uniprot.org, NCBI)
Gene Name
ADRA2A
Full Name
Alpha-2A adrenergic receptor
Weight
50.647kDa
Superfamily
G-protein coupled receptor 1 family
Alternative Names
a-2A AdrenergicR; ADRA2; ADRA2A; ADRA2Ralpha-2-adrenergic receptor, platelet type; ADRARZNF32; adrenergic, alpha-2A-, receptor; Alpha-2 adrenergic receptor subtype C10; alpha-2A Adrenergic R; alpha-2A adrenergic receptor; alpha2A AdrenergicR; Alpha-2A adrenoceptor; Alpha-2A adrenoreceptor; alpha-2AAR subtype C10; ALPHA2AAR; Alpha-2AAR; ZNF32 ADRA2A ADRA2, ADRA2R, ADRAR, ALPHA2AAR, ZNF32 adrenoceptor alpha 2A alpha-2A adrenergic receptor|adrenergic, alpha-2A-, receptor|alpha-2 adrenergic receptor subtype C10|alpha-2-adrenergic receptor, platelet type|alpha-2A adrenoceptor|alpha-2A adrenoreceptor|alpha-2AAR subtype C10
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ADRA2A, check out the ADRA2A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ADRA2A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-alpha 2a Adrenergic Receptor/ADRA2A Antibody Picoband™ (A00883-3)
Hello CJ!
No publications found for A00883-3
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-alpha 2a Adrenergic Receptor/ADRA2A Antibody Picoband™?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-alpha 2a Adrenergic Receptor/ADRA2A Antibody Picoband™
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question