Anti-Calbindin/CALB1 Antibody Picoband™

Boster Bio Anti-Calbindin/CALB1 Antibody Picoband™ catalog # PB9045. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PB9045
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IHC, ICC, WB

Product Name

Anti-Calbindin/CALB1 Antibody Picoband™

See all Calbindin D-28K products

SKU/Catalog Number

PB9045

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Calbindin/CALB1 Antibody Picoband™ catalog # PB9045. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Calbindin/CALB1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9045)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human Calbindin recombinant protein (Position: A2-E175). Human Calbindin shares 99% amino acid (aa) sequence identity with both mouse and rat Calbindin.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PB9045 is reactive to CALB1 in Human, Mouse, Rat

Applications

PB9045 is guaranteed for Flow Cytometry, IHC, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Rat, Human
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For CALB1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P05937

Gene ID

793

Gene Name

CALB1

Full Name

Calbindin

Weight

30.025kDa

Superfamily

calbindin family

Alternative Names

CAB27; CALB; calbindin 1, (28kD); calbindin 1, 28kDa; Calbindin D28; calbindin; D-28K; RTVL-H protein; Vitamin D-dependent calcium-binding protein, avian-type CALB1 CALB, D-28K calbindin 1 calbindin|RTVL-H protein|calbindin 1, (28kD)|calbindin 1, 28kDa|calbindin 27|calbindin D28|vitamin D-dependent calcium-binding protein, avian-type

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CALB1, check out the CALB1 Infographic

CALB1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CALB1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for PB9045

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4 Customer Q&As for Anti-Calbindin/CALB1 Antibody Picoband™

Question

We need using your anti-Calbindin/CALB1 antibody for metanephric part of ureteric bud development studies. Has this antibody been tested with western blotting on siha cells? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-01-30

Answer

Thank you for your inquiry. This PB9045 anti-Calbindin/CALB1 antibody is tested on intestinal cancer tissue, tissue lysate, mammary cancer tissue, mouse kidney tissue, rat kidney tissue, brain tissue, siha cells, hela cells. It is guaranteed to work for Flow Cytometry, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-01-30

Question

Our lab used your anti-Calbindin/CALB1 antibody for Flow Cytometry on caudate nucleus a few months ago. I am using mouse, and I plan to use the antibody for IHC next. I am interested in examining caudate nucleus as well as kidney lung in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?

D. Singh

Verified customer

Asked: 2019-05-20

Answer

I have checked the website and datasheets of our anti-Calbindin/CALB1 antibody and it seems that PB9045 has been validated on mouse in both Flow Cytometry and IHC. Thus PB9045 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-05-20

Question

We have observed staining in human amygdala. Are there any suggestions? Is anti-Calbindin/CALB1 antibody supposed to stain amygdala positively?

Verified Customer

Verified customer

Asked: 2017-08-14

Answer

From literature amygdala does express CALB1. From Uniprot.org, CALB1 is expressed in caudate nucleus, brain, amygdala, cerebellum substantia nigra, kidney lung, among other tissues. Regarding which tissues have CALB1 expression, here are a few articles citing expression in various tissues:
Amygdala, Cerebellum, and Substantia nigra, Pubmed ID: 14702039
Brain, Pubmed ID: 2565876, 3691519
Kidney, and Lung, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2017-08-14

Question

We are currently using anti-Calbindin/CALB1 antibody PB9045 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?

A. Thomas

Verified customer

Asked: 2015-11-25

Answer

The anti-Calbindin/CALB1 antibody (PB9045) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2015-11-25

Conjugation

how to order through PO

Size

Total: $280

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Ships directly to USA and Canada. For other regions, visit our distributors page

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Sample Inquiry

In stock
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PB9045
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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