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Product Info Summary
| SKU: | M11760 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Dog, Human, Monkey, Mouse, Rat |
| Host: | Mouse |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-ALS2CR1 (NIF3L1) Mouse Monoclonal Antibody [Clone ID: OTI5A7]
SKU/Catalog Number
M11760
Size
100 µl
Description
Boster Bio NIF3L1 mouse monoclonal antibody, clone OTI5A7 (formerly 5A7). Catalog# M11760. Tested in IF, IHC, WB. This antibody reacts with Human, Monkey, Mouse, Rat, Dog.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-ALS2CR1 (NIF3L1) Mouse Monoclonal Antibody [Clone ID: OTI5A7] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M11760)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI5A7
Isotype
IgG2a
Immunogen
Full length human recombinant protein of human NIF3L1 (NP_068596) produced in HEK293T cell.
Reactive Species
M11760 is reactive to NIF3L1 in Dog, Human, Monkey, Mouse, Rat
Calculated molecular weight
42.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M11760 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500~2000
IHC 1:150
IF 1:100
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NIF3L1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NIF3L1.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NIF3L1 monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NIF3L1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NIF3L1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NIF3L1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-NIF3L1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-NIF3L1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-NIF3L1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-NIF3L1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NIF3L1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-NIF3L1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Anti-NIF3L1 mouse monoclonal antibody (M11760) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NIF3L1.
Specific Publications For Anti-ALS2CR1 (NIF3L1) Mouse Monoclonal Antibody [Clone ID: OTI5A7] (M11760)
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