Anti-KCC2/SLC12A5 Antibody Picoband™

KCC2/SLC12A5 antibody

Boster Bio Anti-KCC2/SLC12A5 Antibody Picoband™ catalog # A04629-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A04629-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IHC, WB

Product Name

Anti-KCC2/SLC12A5 Antibody Picoband™

View all KCC2/SLC12A5 Antibodies

SKU/Catalog Number

A04629-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-KCC2/SLC12A5 Antibody Picoband™ catalog # A04629-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-KCC2/SLC12A5 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04629-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human KCC2/SLC12A5 recombinant protein (Position: A228-N833).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A04629-1 is reactive to SLC12A5 in Human, Mouse, Rat

Applications

A04629-1 is guaranteed for ELISA, IHC, WB Boster Guarantee

Background of KCC2/SLC12A5

Potassium-chloride transporter member 5 is also known by the names: KCC2 (potassium chloride cotransporter 2) for its ionic substrates, and SLC12A5 for its genetic origin from the SLC12A5 gene in humans. It is mapped to 20q13.12. K-Cl cotransporters are proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The protein encoded by this gene is an integral membrane K-Cl cotransporter that can function in either a net efflux or influx pathway, depending on the chemical concentration gradients of potassium and chloride. The encoded protein can act as a homomultimer, or as a heteromultimer with other K-Cl cotransporters, to maintain chloride homeostasis in neurons. Alternative splicing results in two transcript variants encoding different isoforms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For SLC12A5 (Source: Uniprot.org, NCBI)

Uniprot ID

Q9H2X9

Gene ID

57468

Gene Name

SLC12A5

Full Name

Solute carrier family 12 member 5

Weight

126.184kDa

Superfamily

SLC12A transporter family

Alternative Names

Electroneutral potassium-chloride cotransporter 2; hKCC2; KCC2; KCC2K-Cl cotransporter 2; KIAA1176erythroid K-Cl cotransporter 2; Neuronal K-Cl cotransporter; SLC12A5; solute carrier family 12 (potassium/chloride transporter), member 5; solute carrier family 12 member 5 SLC12A5 DEE34, EIEE34, EIG14, KCC2, hKCC2 solute carrier family 12 member 5 solute carrier family 12 member 5|K-Cl cotransporter 2|electroneutral potassium-chloride cotransporter 2|erythroid K-Cl cotransporter 2|neuronal K-Cl cotransporter|solute carrier family 12 (potassium/chloride transporter), member 5

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SLC12A5, check out the SLC12A5 Infographic

SLC12A5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SLC12A5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

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1 Customer Q&As for Anti-KCC2/SLC12A5 Antibody Picoband™

Question

We are currently using anti-KCC2/SLC12A5 antibody A04629-1 for human tissue, and we are satisfied with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?

T. Huang

Verified customer

Asked: 2019-08-22

Answer

The anti-KCC2/SLC12A5 antibody (A04629-1) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-22

Size

Conjugation

Total: $315

SKU:A04629-1

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Ships directly to USA and Canada. For other regions, visit our distributors page

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Sample Inquiry

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A04629-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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