Anti-Ambra1 Antibody

AMBRA1 antibody

Boster Bio Anti-Ambra1 Antibody (Catalog # A03799). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A03799
Size: 0.1 mg
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IF, IHC-P, WB

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Product Name

Anti-Ambra1 Antibody

View all AMBRA1 Antibodies

SKU/Catalog Number

A03799

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Ambra1 Antibody (Catalog # A03799). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Ambra1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Ambra1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03799)

Host

Rabbit

Contents

Ambra1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Ambra1 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human Ambra1. The immunogen is located within the last 50 amino acids of Ambra1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A03799 is reactive to AMBRA1 in Human, Mouse, Rat

Applications

A03799 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

142.507kDa

Background of AMBRA1

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. Beclin-1, a principal regulator of autophagosome formation, is in turn regulated by Ambra1. Ambra1 associates with Beclin-1 through a region near its center as determined by yeast two-hybrid assay. Null mutations in this gene in mice resulted in embryonic lethality with severe neural tube defects associated with autophagy impairment, accumulation of ubiquitinated proteins, unbalanced cell proliferation and excessive apoptotic death. Furthermore, down-regulation of Ambra1 in cultured cells though RNA interference decreased the level of rapamycin- and nutrient starvation-induced autophagy. Multiple isoforms of Ambra1 are known to exist.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Ambra1 antibody can be used for the detection of Ambra1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For AMBRA1 (Source: Uniprot.org, NCBI)

Gene Name

AMBRA1

Full Name

Activating molecule in BECN1-regulated autophagy protein 1

Weight

142.507kDa

Alternative Names

activating molecule in BECN1-regulated autophagy protein 1; autophagy/beclin-1 regulator 1; DCAF3; DDB1 and CUL4 associated factor 3; FLJ20294; KIAA1736activating molecule in beclin-1-regulated autophagy; MGC33725; WD repeat domain 94; WDR94 AMBRA1 DCAF3, WDR94 autophagy and beclin 1 regulator 1 activating molecule in BECN1-regulated autophagy protein 1|DDB1 and CUL4 associated factor 3|WD repeat domain 94|activating molecule in beclin-1-regulated autophagy|autophagy/beclin-1 regulator 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on AMBRA1, check out the AMBRA1 Infographic

AMBRA1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for AMBRA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Size

Total: $449

SKU:A03799

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Lead time for this item is typically 4-7 days

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A03799
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$449.00

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