Product Info Summary
SKU: | A03799 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, IHC-P, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Ambra1 Antibody
SKU/Catalog Number
A03799
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Ambra1 Antibody (Catalog # A03799). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Ambra1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Ambra1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03799)
Host
Rabbit
Contents
Ambra1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Ambra1 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human Ambra1. The immunogen is located within the last 50 amino acids of Ambra1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03799 is reactive to AMBRA1 in Human, Mouse, Rat
Applications
A03799 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
142.507kDa
Background of AMBRA1
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. Beclin-1, a principal regulator of autophagosome formation, is in turn regulated by Ambra1. Ambra1 associates with Beclin-1 through a region near its center as determined by yeast two-hybrid assay. Null mutations in this gene in mice resulted in embryonic lethality with severe neural tube defects associated with autophagy impairment, accumulation of ubiquitinated proteins, unbalanced cell proliferation and excessive apoptotic death. Furthermore, down-regulation of Ambra1 in cultured cells though RNA interference decreased the level of rapamycin- and nutrient starvation-induced autophagy. Multiple isoforms of Ambra1 are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Ambra1 antibody can be used for the detection of Ambra1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Ambra1 in 3T3 cell lysate with Ambra1 antibody at 1 μg/mL.
Click image to see more details
Immunohistochemistry of Ambra1 in human brain with Ambra1 antibody at 5 μg/mL.
Click image to see more details
Immunofluorescence of Ambra1 in Human Brain cells with Ambra1 antibody at 20 μg/mL.
Click image to see more details
Immunofluorescence of Ambra1 in K562 cells with Ambra1 Antibody at 20 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For AMBRA1 (Source: Uniprot.org, NCBI)
Gene Name
AMBRA1
Full Name
Activating molecule in BECN1-regulated autophagy protein 1
Weight
142.507kDa
Alternative Names
activating molecule in BECN1-regulated autophagy protein 1; autophagy/beclin-1 regulator 1; DCAF3; DDB1 and CUL4 associated factor 3; FLJ20294; KIAA1736activating molecule in beclin-1-regulated autophagy; MGC33725; WD repeat domain 94; WDR94 AMBRA1 DCAF3, WDR94 autophagy and beclin 1 regulator 1 activating molecule in BECN1-regulated autophagy protein 1|DDB1 and CUL4 associated factor 3|WD repeat domain 94|activating molecule in beclin-1-regulated autophagy|autophagy/beclin-1 regulator 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AMBRA1, check out the AMBRA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AMBRA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ambra1 Antibody (A03799)
Hello CJ!
No publications found for A03799
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Ambra1 Antibody?
Submit a review and receive an Amazon gift card.
- $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Submit A Review
Be the first to review Anti-Ambra1 Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question