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Product Info Summary
| SKU: | A02929 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-ANGPTL3 Antibody Picoband®
SKU/Catalog Number
A02929
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ANGPTL3 Antibody Picoband® catalog # A02929. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ANGPTL3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02929)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human ANGPTL3, identical to the related mouse and rat sequences.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02929 is reactive to ANGPTL3 in Human, Mouse, Rat
Observed Molecular Weight
54 kDa
Calculated molecular weight
53.6 kDa
Background of ANGPTL3
ANGPTL3 (Angiopoietin-Like 3), also known as ANGPT5, is a protein which in humans is encoded by the ANGPTL3 gene. The protein encoded by this gene is a member of the angiopoietin-like family of secreted factors. By radiation hybrid mapping and the use of surrounding genes, this gene is mapped to chromosome 1p31. It is predominantly expressed in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain and the C-terminal fibrinogen (FBN)-like domain. Angptl3 also acts as dual inhibitor of lipoprotein lipase (LPL) and endothelial lipase (EL), and increases plasma triglyceride and HDL cholesterol in rodents. ANGPTL3 inhibit endothelial lipase to catalyze HDL-phospholipid and increase HDL-PL levels.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02929 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Positive Control
WB: mouse HEPA1-6 cell
Validation Images & Assay Conditions
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Western blot analysis of ANGPTL3 using anti-ANGPTL3 antibody (A02929).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse HEPA1-6 cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ANGPTL3 antigen affinity purified polyclonal antibody (Catalog # A02929) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ANGPTL3 at approximately 54KD. The expected band size for ANGPTL3 is at 54KD.
Specific Publications For Anti-ANGPTL3 Antibody Picoband® (A02929)
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Customer Q&As
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3 Customer Q&As for Anti-ANGPTL3 Antibody Picoband®
Question
We have seen staining in mouse plasma. Do you have any suggestions? Is anti-ANGPTL3 antibody supposed to stain plasma positively?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
Based on literature plasma does express ANGPTL3. Based on Uniprot.org, ANGPTL3 is expressed in liver, liver skeletal muscle, plasma, among other tissues. Regarding which tissues have ANGPTL3 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 10644446, 14702039, 19159218
Liver, and Skeletal muscle, Pubmed ID: 15489334
Plasma, Pubmed ID: 16335952
Boster Scientific Support
Answered: 2019-08-22
Question
We are currently using anti-ANGPTL3 antibody A02929 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
K. Jha
Verified customer
Asked: 2019-06-20
Answer
The anti-ANGPTL3 antibody (A02929) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-20
Question
Our lab were happy with the WB result of your anti-ANGPTL3 antibody. However we have been able to see positive staining in liver secreted using this antibody. Is that expected? Could you tell me where is ANGPTL3 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-01-04
Answer
According to literature, liver does express ANGPTL3. Generally ANGPTL3 expresses in secreted. Regarding which tissues have ANGPTL3 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 10644446, 14702039, 19159218
Liver, and Skeletal muscle, Pubmed ID: 15489334
Plasma, Pubmed ID: 16335952
Boster Scientific Support
Answered: 2019-01-04
