Anti-PCSK9 Antibody

Boster Bio Anti-PCSK9 Antibody catalog # PA2130. Tested in IHC, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: PA2130
Size: 100μg/vial
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

Product Name

Anti-PCSK9 Antibody

See all Proprotein Convertase 9/PCSK9 products

SKU/Catalog Number

PA2130

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PCSK9 Antibody catalog # PA2130. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PCSK9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2130)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human PCSK9 (671-687aa AVTAVAICCRSRHLAQA).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA2130 is reactive to PCSK9 in Human

Applications

PA2130 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For PCSK9 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q8NBP7

Gene ID

255738

Gene Name

PCSK9

Full Name

Proprotein convertase subtilisin/kexin type 9

Weight

74.286kDa

Superfamily

peptidase S8 family

Alternative Names

EC 3.4.21; EC 3.4.21.111; FH3; FH3neural apoptosis regulated convertase 1; FHCL3; HCHOLA3; hypercholesterolemia, autosomal dominant 3; LDLCQ1; NARC1; NARC-1; NARC-1convertase subtilisin/kexin type 9 preproprotein; NARC1EC 3.4.21.-; Neural apoptosis-regulated convertase 1; PC9; PCSK9; Proprotein Convertase 9; proprotein convertase subtilisin/kexin type 9; Subtilisin/kexin-like protease PC9 PCSK9 FH3, FHCL3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9 proprotein convertase subtilisin/kexin type 9 proprotein convertase subtilisin/kexin type 9|convertase subtilisin/kexin type 9 preproprotein|neural apoptosis regulated convertase 1|subtilisin/kexin-like protease PC9

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on PCSK9, check out the PCSK9 Infographic

PCSK9 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for PCSK9: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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17 Customer Q&As for Anti-PCSK9 Antibody

Question

Is there a BSA free version of anti-PCSK9 antibody PA2130 available?

Verified Customer

Verified customer

Asked: 2020-04-23

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PCSK9 antibody PA2130 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-04-23

Question

We have observed staining in human cervix carcinoma erythroleukemia. Do you have any suggestions? Is anti-PCSK9 antibody supposed to stain cervix carcinoma erythroleukemia positively?

Verified Customer

Verified customer

Asked: 2019-10-24

Answer

From what I have seen in literature cervix carcinoma erythroleukemia does express PCSK9. From what I have seen in Uniprot.org, PCSK9 is expressed in right lobe of liver, cerebellum teratocarcinoma, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have PCSK9 expression, here are a few articles citing expression in various tissues:
Cerebellum, and Teratocarcinoma, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163

Boster Scientific Support

Answered: 2019-10-24

Question

My lab would like using your anti-PCSK9 antibody for low-density lipoprotein particle clearance studies. Has this antibody been tested with western blotting on hela whole cell lysate? We would like to see some validation images before ordering.

S. Taylor

Verified customer

Asked: 2019-10-11

Answer

Thank you for your inquiry. This PA2130 anti-PCSK9 antibody is validated on a549 whole cell lysate, hela whole cell lysate, u87 whole cell lysate, panc whole cell lysate, intestinal cancer tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-10-11

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-PCSK9 antibody PA2130. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-10-07

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-07

Question

I was wanting to use your anti-PCSK9 antibody for IHC for human cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix carcinoma identification?

Verified Customer

Verified customer

Asked: 2019-09-17

Answer

As indicated on the product datasheet, PA2130 anti-PCSK9 antibody has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-09-17

Question

Would anti-PCSK9 antibody PA2130 work on dog WB with right lobe of liver?

Verified Customer

Verified customer

Asked: 2019-09-04

Answer

Our lab technicians have not validated anti-PCSK9 antibody PA2130 on dog. You can run a BLAST between dog and the immunogen sequence of anti-PCSK9 antibody PA2130 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog right lobe of liver in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-09-04

Question

Is a blocking peptide available for product anti-PCSK9 antibody (PA2130)?

Verified Customer

Verified customer

Asked: 2019-08-28

Answer

We do provide the blocking peptide for product anti-PCSK9 antibody (PA2130). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-08-28

Question

Would anti-PCSK9 antibody PA2130 work for IHC with cervix carcinoma?

Verified Customer

Verified customer

Asked: 2019-04-17

Answer

According to the expression profile of cervix carcinoma, PCSK9 is highly expressed in cervix carcinoma. So, it is likely that anti-PCSK9 antibody PA2130 will work for IHC with cervix carcinoma.

Boster Scientific Support

Answered: 2019-04-17

Question

My question regarding product PA2130, anti-PCSK9 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-01-01

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2130 anti-PCSK9 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-01-01

Question

I see that the anti-PCSK9 antibody PA2130 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-11-26

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-11-26

Question

See below the WB image, lot number and protocol we used for cervix carcinoma using anti-PCSK9 antibody PA2130. Please let me know if you require anything else.

G. Edwards

Verified customer

Asked: 2018-10-15

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-10-15

Question

Does PA2130 anti-PCSK9 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2017-07-13

Answer

You can see on the product datasheet, PA2130 anti-PCSK9 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-07-13

Question

My colleagues were well pleased with the WB result of your anti-PCSK9 antibody. However we have observed positive staining in cerebellum teratocarcinoma cytoplasm. secreted. endosome. lysosome. using this antibody. Is that expected? Could you tell me where is PCSK9 supposed to be expressed?

D. Dhar

Verified customer

Asked: 2017-03-20

Answer

From literature, cerebellum teratocarcinoma does express PCSK9. Generally PCSK9 expresses in cytoplasm. secreted. endosome. lysosome. Regarding which tissues have PCSK9 expression, here are a few articles citing expression in various tissues:
Cerebellum, and Teratocarcinoma, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163

Boster Scientific Support

Answered: 2017-03-20

Question

Is this PA2130 anti-PCSK9 antibody reactive to the isotypes of PCSK9?

S. Dhar

Verified customer

Asked: 2016-03-21

Answer

The immunogen of PA2130 anti-PCSK9 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human PCSK9(671-687aa AVTAVAICCRSRHLAQA). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2016-03-21

Question

We ordered your anti-PCSK9 antibody for WB on cervix carcinoma in a previous experiment. I am using human, and I plan to use the antibody for IHC next. I am looking for examining cervix carcinoma as well as cervix carcinoma erythroleukemia in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?

M. Edwards

Verified customer

Asked: 2014-12-02

Answer

I have checked the website and datasheets of our anti-PCSK9 antibody and it appears that PA2130 has been validated on human in both WB and IHC. Thus PA2130 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2014-12-02

Question

We need to test anti-PCSK9 antibody PA2130 on human cervix carcinoma for research purposes, then I may be interested in using anti-PCSK9 antibody PA2130 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

D. Krishna

Verified customer

Asked: 2014-08-04

Answer

The products we sell, including anti-PCSK9 antibody PA2130, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2014-08-04

Question

We are currently using anti-PCSK9 antibody PA2130 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?

V. Banerjee

Verified customer

Asked: 2014-02-10

Answer

The anti-PCSK9 antibody (PA2130) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-02-10

Size

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PA2130
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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