Anti-Annexin VI/ANXA6 Antibody
Rabbit IgG polyclonal antibody for Annexin A6(ANXA6) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;Rat.
|Reactivity||Human, Mouse, Rat|
|Product Name||Anti-Annexin VI/ANXA6 Antibody
See all ANXA6 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for ANNEXIN VI/ANXA6 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. ANNEXIN VI/ANXA6 information: Molecular Weight: 75873 MW; Subcellular Localization: Cytoplasm . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV.|
|Cite This Product||Anti-Annexin VI/ANXA6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1436)|
|Specificity||Anti-Annexin VI/ANXA6 Antibody (PA1436) reacts with Human, Mouse, Rat ANXA6, in native form and recombinant. Superfamily members of ANXA6 are not reactive to PA1436.|
|Contents/Buffer||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Annexin VI(636-652aa NIRREFIEKYDKSLHQA), identical to the related mouse and rat sequences.|
|Reactivity||Human, Mouse, Rat|
Our Boster Quality Guarantee for Anti-Annexin VI/ANXA6 Antibody covers its use in the following applications.
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Assay Dilutions Overview
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Rat, Human, Mouse
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Boster's Compatible Products
The following reagents are used to generate the images below for Anti-Annexin VI/ANXA6 Antibody (PA1436).Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F).
Images And Assay Conditions
Anti-Annexin VI antibody, PA1436, Western blotting
Lane 1: Rat Ovary Tissue Lysate
Lane 2: Rat Liver Tissue Lysate
Lane 3: Rat Intestinum Tenue Tissue Lysate
Lane 4: Rat Brain Tissue Lysate
Lane 5: A549 Cell Lysate
Lane 6: JURKAT Cell Lysate
Lane 7: RAJI Cell Lysate
Lane 8: CEM Cell Lysate
Lane 9: SMMC Cell Lysate
Anti-Annexin VI antibody, PA1436, IHC(P)
IHC(P): Rat Spleen Tissue
Anti-Annexin VI antibody, PA1436, IHC(F)
IHC(F): Rat Spleen Tissue
Protein Target Info (Source: Uniprot.org)
|Protein Name||Annexin A6|
|Alternative Names||Annexin A6;67 kDa calelectrin;Annexin VI;Annexin-6;Calphobindin-II;CPB-II;Chromobindin-20;Lipocortin VI;Protein III;p68;p70;ANXA6;ANX6;|
|Subcellular Localization||Cytoplasm . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV.|
|Molecular Weight||75873 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||May associate with CD21. May regulate the release of Ca(2+) from intracellular stores.|
|Research Areas||Human, Mouse, Rat
*You can search these to find other products in these research areas.
|Background||Annexin A6(ANXA6) is a member of a family of proteins that bind membrane or cytoskeleton in a Ca(2+)-dependent manner. These proteins are characterized by homologous amino acid sequences that are present in multiple copies in each protein. ANXA6 gene is assigned to 5q32-q34 by use of a cDNA clone to probe genomic DNA from rodent-human somatic cell hybrids and for in situ hybridization.The ANX6 gene is approximately 60 kb long and contains 26 exons. The genomic sequence at the 3-prime end does not contain a canonical polyadenylylation signal.Ca(2+)-dependent binding between CRHSP28 and ANXA6 is required for acinar cell membrane trafficking events and digestive enzyme secretion|
Other Recommended Resources
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Publishing with Anti-Annexin VI/ANXA6 Antibody (PA1436)? Please let us know so we can cite the reference in this product datasheet. Email us at [email protected]
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugationA: Yes, please contact us at [email protected] for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WBA: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugationA: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact [email protected]
Q: What should I use for negative control?A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signalA: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?A: You can find the immunogen sequence under "
Q: What is the expected band size? Why is it different than the observed band size?A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?A: Some common names include but are not limited to p68 antibody, annexin vi antibody