Product Info Summary
| SKU: | PA1436 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Annexin VI/ANXA6 Antibody Picoband®
SKU/Catalog Number
PA1436
BA3110 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Annexin VI/ANXA6 Antibody catalog # PA1436. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Annexin VI/ANXA6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1436)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Annexin VI, identical to the related mouse and rat sequences.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1436 is reactive to ANXA6 in Human, Mouse, Rat
Observed Molecular Weight
45 kDa
Calculated molecular weight
75.9 kDa
Background of ANXA6
Annexin A6 (ANXA6) is a member of a family of proteins that bind membrane or cytoskeleton in a Ca (2+)-dependent manner. These proteins are characterized by homologous amino acid sequences that are present in multiple copies in each protein. ANXA6 gene is assigned to 5q32-q34 by use of a cDNA clone to probe genomic DNA from rodent-human somatic cell hybrids and for in situ hybridization.The ANX6 gene is approximately 60 kb long and contains 26 exons. The genomic sequence at the 3-prime end does not contain a canonical polyadenylylation signal.Ca (2+)-dependent binding between CRHSP28 and ANXA6 is required for acinar cell membrane trafficking events and digestive enzyme secretion
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1436 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat, Human, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: Rat Ovary Tissue, Rat Liver Tissue, Rat Intestinum Tenue Tissue, Rat Brain Tissue, A549 Cell, JURKAT Cell, RAJI Cell, CEM Cell, SMMC Cell
IHC: Rat Spleen Tissue
IHC-F: Rat Spleen Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-Annexin VI antibody, PA1436, Western blotting
Lane 1: Rat Ovary Tissue Lysate
Lane 2: Rat Liver Tissue Lysate
Lane 3: Rat Intestinum Tenue Tissue Lysate
Lane 4: Rat Brain Tissue Lysate
Lane 5: A549 Cell Lysate
Lane 6: JURKAT Cell Lysate
Lane 7: RAJI Cell Lysate
Lane 8: CEM Cell Lysate
Lane 9: SMMC Cell Lysate
Click image to see more details
Anti-Annexin VI antibody, PA1436, IHC(P)
IHC(P): Rat Spleen Tissue
Click image to see more details
Anti-Annexin VI antibody, PA1436, IHC(F)
IHC(F): Rat Spleen Tissue
Specific Publications For Anti-Annexin VI/ANXA6 Antibody Picoband® (PA1436)
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Customer Q&As
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3 Customer Q&As for Anti-Annexin VI/ANXA6 Antibody Picoband®
Question
Our lab want to know about to test anti-Annexin VI/ANXA6 antibody PA1436 on rat liver for research purposes, then I may be interested in using anti-Annexin VI/ANXA6 antibody PA1436 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Roberts
Verified customer
Asked: 2020-03-05
Answer
The products we sell, including anti-Annexin VI/ANXA6 antibody PA1436, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-05
Question
Will PA1436 anti-Annexin VI/ANXA6 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
It shows on the product datasheet, PA1436 anti-Annexin VI/ANXA6 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-21
Question
We are currently using anti-Annexin VI/ANXA6 antibody PA1436 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?
D. Gonzalez
Verified customer
Asked: 2013-05-02
Answer
The anti-Annexin VI/ANXA6 antibody (PA1436) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-05-02


