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Product Info Summary
| SKU: | PA2076 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Annexin VII/ANXA7 Antibody Picoband®
SKU/Catalog Number
PA2076
BA4027 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Annexin VII/ANXA7 Antibody catalog # PA2076. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Annexin VII/ANXA7 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2076)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Annexin VII, different from the related mouse and rat sequences by one amino acid.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2076 is reactive to ANXA7 in Human
Observed Molecular Weight
50 kDa
Calculated molecular weight
52.7 kDa
Background of ANXA7
ANXA7 (Annexin A7), also known as ANX7 or SNX, is a protein that in humans is encoded by the ANXA7 gene. Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins. Shirvan et al. (1994) isolated genomic clones of the ANXA7 gene and assigned the gene to 10q21.1-q21.2 by study of somatic cell hybrids and by in situ hybridization. Srivastava et al. (2001) concluded that the ANX7 gene exhibits many biologic and genetic properties expected of a tumor suppressor gene and may play a role in prostate cancer progression. Caohuy et al. (1996) present experimental evidence, based on studies of recombinant human ANXA7 and isolated bovine chromaffin cells, that ANXA7 is a Ca (2+)-dependent GTP binding protein. ANXA7 was active in a chromaffin granule aggregation assays in the presence of Ca (2+) and GTP, and was deactivated upon GTP hydrolysis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2076 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: human Jurkat whole cell, human SW620 whole cell, human K562 whole cell, human HepG2 whole cell, human A549 whole cell, human Raji whole cell, human HEK293 whole cell
IHC: Human Mammary Cancer tissue
ICC/IF: A431 cell
FCM: HepG2 cell
Validation Images & Assay Conditions
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Western blot analysis of ANXA7 using anti-ANXA7 antibody (PA2076).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human SW620 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: human A549 whole cell lysates,
Lane 6: human Raji whole cell lysates,
Lane 7: human HEK293 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ANXA7 antigen affinity purified polyclonal antibody (Catalog # PA2076) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ANXA7 at approximately 50 kDa. The expected band size for ANXA7 is at 53 kDa.
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Flow Cytometry analysis of HepG2 cells using anti-ANXA7 antibody (PA2076).
Overlay histogram showing HepG2 cells stained with PA2076 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ANXA7 Antibody (PA2076, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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IHC analysis of ANXA7 using anti-ANXA7 antibody (PA2076).
ANXA7 was detected in a paraffin-embedded section of Human Mammary Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ANXA7 Antibody (PA2076) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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IF analysis of ANXA7 using anti- ANXA7 antibody (PA2076).
ANXA7 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- ANXA7 Antibody (PA2076) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Specific Publications For Anti-Annexin VII/ANXA7 Antibody Picoband® (PA2076)
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Customer Q&As
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4 Customer Q&As for Anti-Annexin VII/ANXA7 Antibody Picoband®
Question
Will anti-Annexin VII/ANXA7 antibody PA2076 work for IHC with liver?
Verified Customer
Verified customer
Asked: 2019-03-05
Answer
According to the expression profile of liver, ANXA7 is highly expressed in liver. So, it is likely that anti-Annexin VII/ANXA7 antibody PA2076 will work for IHC with liver.
Boster Scientific Support
Answered: 2019-03-05
Question
Will PA2076 anti-Annexin VII/ANXA7 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
O. Patel
Verified customer
Asked: 2016-12-07
Answer
As indicated on the product datasheet, PA2076 anti-Annexin VII/ANXA7 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-12-07
Question
I see that the anti-Annexin VII/ANXA7 antibody PA2076 works with IHC, what is the protocol used to produce the result images on the product page?
Z. Jha
Verified customer
Asked: 2016-04-26
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2016-04-26
Question
We are currently using anti-Annexin VII/ANXA7 antibody PA2076 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
T. Miller
Verified customer
Asked: 2016-03-09
Answer
The anti-Annexin VII/ANXA7 antibody (PA2076) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-03-09
