Rabbit IgG polyclonal antibody for Apelin(APLN) detection. Tested with WB in Human;Mouse;Rat.
Anti-Apelin/APLN Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Product Name||Anti-Apelin/APLN Antibody
See all Apelin primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for APELIN/APLN detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. APELIN/APLN information: Molecular Weight: 8569 MW; Subcellular Localization: Secreted; Tissue Specificity: Expressed in the brain with highest levels in the frontal cortex, thalamus, hypothalamus and midbrain. Secreted by the mammary gland into the colostrum and the milk.|
|Cite This Product||Anti-Apelin/APLN Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1501)|
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|Anti-Apelin/APLN Antibody may replace the following items: sc 33469|sc 33470|sc 33804|sc 33805.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Apelin(60-77aa RKFRRQRPRLSHKGPMPF), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For APLN (Source: Uniprot.org, NCBI)
|NCBI Gene Id||8862|
|Species Of This Entry||Human|
|Alternative Names||Apelin|AGTRL1 ligand; AGTRL1 Lligand; APEL; Apelin; apelin, AGTRL1 ligand; APJ endogenous ligand; APLN; XNPEP2|
|Post Tranlational Modifications||Phosphorylation; Glycosylation; Oxidation; Nitration; Reduction; Cleavage; Acylation|
|Subcellular Localization||Secreted. Secreted, extracellular space. Abundantly secreted in the colostrum. Lower level in milk. Decreases rapidly within several days after parturition in milk, but is still detectable even in commercial milk.|
|Gene Location||Xq26.1, on Chromasome X, gene sequence: NC_000023.11 (129645259..129654956, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For APLN (Source: Uniprot.org, NCBI)
|Protein Function||Endogenous ligand for the apelin receptor (APLNR) (PubMed:10525157). Drives internalization of the apelin receptor (By similarity). Apelin-36 dissociates more hardly than (pyroglu)apelin-13 from APLNR (By similarity). Hormone involved in the regulation of cardiac precursor cell movements during gastrulation and heart morphogenesis (By similarity). Has an inhibitory effect on cytokine production in response to T-cell receptor/CD3 cross-linking; the oral intake of apelin in the colostrum and the milk might therefore modulate immune responses in neonates (By similarity). Plays a role in early coronary blood vessels formation (By similarity). Mediates myocardial contractility in an ERK1/2-dependent manner (By similarity). May also have a role in the central control of body fluid homeostasis by influencing vasopressin release and drinking behavior (By similarity).|
|Tissue Specificity||Expressed in the brain with highest levels in the frontal cortex, thalamus, hypothalamus and midbrain (PubMed:10617103). Secreted by the mammary gland into the colostrum and the milk.|
|Related Diseases||Obesity; Heart Failure; Tumor Angiogenesis; Insulin Resistance; Diabetes Mellitus; Hypertensive Disease; Cardiovascular Diseases; Diabetes Mellitus, Non-insulin-dependent; Inflammation; Pathologic Neovascularization; Nervousness; Atherosclerosis; Hypoxia; Insulin Sensitivity; Hiv Infections; Hypertrophy; Infarction; Immunologic Deficiency Syndromes; Myocardial Infarction; Pituitary Diseases|
|Related Pathways||Angiogenesis; Secretion; Pathogenesis; Cell Proliferation; Localization; Glucose Homeostasis; Vasoconstriction; Regulation Of Blood Pressure; Diuresis; Insulin Secretion; Lactation; Regeneration; Receptor Internalization; Cellular Localization; Feeding Behavior; Vasodilation; Heart Development; Neuroprotection; Cell Migration; Vasculogenesis|
|Background||Apelin is a neuropeptide expressed in the supraoptic and paraventricular nuclei that acts on specific receptors located on vasopressinergic neurons. The apelin gene to Xq25-q26.3 based on sequence similarity between the apelin sequence and a chromosome X PAC clone. This gene encodes a peptide that functions as an endogenous ligand for the G protein coupled receptor APJ. The encoded protein is synthesized as a prepropeptide that is processed into biologically active C-terminal fragments. The peptide fragments activate different tissue specific signaling pathways that regulate diverse biological functions including fluid homeostasis, cardiovascular function and insulin secretion. This protein also functions as a coreceptor for the human immunodeficiency virus 1.|
|Scientific References||PMID: 9792798 by Tatemoto K., et al. Isolation and characterization of a novel endogenous peptide ligand for the human APJ receptor.|
PMID: 10525157 by Habata Y., et al. Apelin, the natural ligand of the orphan receptor APJ, is abundantly secreted in the colostrum.
Our Boster Quality Guarantee for Anti-Apelin/APLN Antibody covers its use in the following applications.Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Working concentration of primary antibody: 0.5 μg/ml; 40μg protein was loaded.
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