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Product Info Summary
| SKU: | A00472 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-ApoA LPA Monoclonal Antibody
SKU/Catalog Number
A00472
Size
100μl
Form
Liquid
Description
Boster Bio Anti-ApoA LPA Monoclonal Antibody catalog # A00472. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ApoA LPA Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00472)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
8F6A9; 8H5C5
Isotype
IgG
Immunogen
Purified recombinant fragment of ApoA (4330-4521) expressed in E. Coli.
Reactive Species
A00472 is reactive to LPA in Human
Observed Molecular Weight
39 kDa
Calculated molecular weight
501.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00472 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
ELISA 1:10000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot (WB) analysis using ApoA Monoclonal antibody against truncated LPA-His recombinant protein (1) and truncated Trx-LPA(aa4330-4521) recombinant protein (2).
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Immunohistochemistry (IHC) analysis of paraffin-embedded Human Liver Tissues with AEC staining using ApoA Monoclonal antibody.
Specific Publications For Anti-ApoA LPA Monoclonal Antibody (A00472)
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Customer Q&As
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3 Customer Q&As for Anti-ApoA LPA Monoclonal Antibody
Question
We are currently using anti-ApoA Monoclonal antibody A00472 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?
Z. Li
Verified customer
Asked: 2019-09-03
Answer
The anti-ApoA Monoclonal antibody (A00472) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-03
Question
Our lab want to know about to test anti-ApoA Monoclonal antibody A00472 on human lymphocyte for research purposes, then I may be interested in using anti-ApoA Monoclonal antibody A00472 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
T. Walker
Verified customer
Asked: 2019-08-30
Answer
The products we sell, including anti-ApoA Monoclonal antibody A00472, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-30
Question
Will anti-ApoA Monoclonal antibody A00472 work on pig IHC with lymphocyte?
Verified Customer
Verified customer
Asked: 2018-03-23
Answer
Our lab technicians have not validated anti-ApoA Monoclonal antibody A00472 on pig. You can run a BLAST between pig and the immunogen sequence of anti-ApoA Monoclonal antibody A00472 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig lymphocyte in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-03-23
