Anti-Apoptosis inhibitor 5/API5 Antibody

Boster Bio Anti-Apoptosis inhibitor 5/API5 Antibody catalog # PA1009. Tested in IHC-P, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PA1009
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC-P, IHC-F, ICC, WB

Product Name

Anti-Apoptosis inhibitor 5/API5 Antibody

See all API5 products

SKU/Catalog Number

PA1009

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Apoptosis inhibitor 5/API5 Antibody catalog # PA1009. Tested in IHC-P, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Apoptosis inhibitor 5/API5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1009)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Apoptosis inhibitor 5 (487-504aa KYSSNLGNFNYERSLQGK) (b), different from the related mouse sequence by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1009 is reactive to API5 in Human, Mouse, Rat

Applications

PA1009 is guaranteed for IHC-P, IHC-F, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunocytochemistry , 0.5-1μg/ml, Human, Mouse
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat, Human, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For API5 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9BZZ5

Gene ID

8539

Gene Name

API5

Full Name

Apoptosis inhibitor 5

Weight

59.005kDa

Superfamily

API5 family

Alternative Names

AAC-11API5L1; AAC11fibroblast growth factor 2-interacting factor 2; Antiapoptosis clone 11 protein; API-5; apoptosis inhibitor 5; Cell migration-inducing gene 8 protein; Fibroblast growth factor 2-interacting factor; FIF; migration-inducing protein MIG8; Protein XAGL API5 AAC-11, AAC11 apoptosis inhibitor 5 apoptosis inhibitor 5|FIF|antiapoptosis clone 11 protein|cell migration-inducing gene 8 protein|fibroblast growth factor 2-interacting factor 2|migration-inducing protein MIG8

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on API5, check out the API5 Infographic

API5 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for API5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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6 Customer Q&As for Anti-Apoptosis inhibitor 5/API5 Antibody

Question

Would PA1009 anti-Apoptosis inhibitor 5/API5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-01-28

Answer

You can see on the product datasheet, PA1009 anti-Apoptosis inhibitor 5/API5 antibody as been validated on IHC-F. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-01-28

Question

We are currently using anti-Apoptosis inhibitor 5/API5 antibody PA1009 for mouse tissue, and we are well pleased with the IHC-F results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-02

Answer

The anti-Apoptosis inhibitor 5/API5 antibody (PA1009) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-02

Question

Is this PA1009 anti-Apoptosis inhibitor 5/API5 antibody reactive to the isotypes of API5?

B. Parker

Verified customer

Asked: 2018-11-15

Answer

The immunogen of PA1009 anti-Apoptosis inhibitor 5/API5 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Apoptosis inhibitor 5(487-504aa KYSSNLGNFNYERSLQGK)(b), different from the related mouse sequence by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-11-15

Question

Does anti-Apoptosis inhibitor 5/API5 antibody PA1009 work on primate IHC-F with ovarian carcinoma?

Verified Customer

Verified customer

Asked: 2018-11-14

Answer

Our lab technicians have not validated anti-Apoptosis inhibitor 5/API5 antibody PA1009 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Apoptosis inhibitor 5/API5 antibody PA1009 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate ovarian carcinoma in IHC-F, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-11-14

Question

I have attached the WB image, lot number and protocol we used for colon carcinoma using anti-Apoptosis inhibitor 5/API5 antibody PA1009. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-04-16

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-04-16

Question

Do you have a BSA free version of anti-Apoptosis inhibitor 5/API5 antibody PA1009 available?

Verified Customer

Verified customer

Asked: 2017-10-24

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Apoptosis inhibitor 5/API5 antibody PA1009 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-10-24

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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Sample Inquiry

In stock
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PA1009
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

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