Product Info Summary
SKU: | PA1742 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-Aquaporin 2/AQP2 Antibody
View all Aquaporin-2 Antibodies
SKU/Catalog Number
PA1742
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Aquaporin 2/AQP2 Antibody catalog # PA1742. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Aquaporin 2/AQP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1742)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 2, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1742 is reactive to AQP2 in Human, Mouse, Rat
Applications
PA1742 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
65 kDa
Calculated molecular weight
28.837kDa
Background of Aquaporin-2
AQP2 (Aquaporin 2) also called AQUAPORIN-CD, is found in the apical cell membranes of the kidney's collecting duct principal cells and in intracellular vesicles located throughout the cell. The AQP2 gene is mapped to chromosome 12q13, very close to the site of major intrinsic protein by situ hybridization. The investigators suggested that a defect in the AQP2 gene is the basis of the autosomal form of nephrogenic diabetes insipidus. The functional expression and the limited localization suggested that AQP2 is the vasopressin-regulated water channel. Using rat kidney slices and porcine kidney cells stably expressing rat Aqp2, AQP2 trafficking can be stimulated by cAMP-independent pathways that utilize nitric oxide (NO). The NO donors sodium nitroprusside (SNP) and NONOate and the NO synthase substrate L-arginine mimicked the effect of vasopressin (VP), stimulating relocation of Aqp2 from cytoplasmic vesicles to the apical plasma membrane. SNP increased intracellular cGMP rather than cAMP, and exogenous cGMP stimulated AQP2 membrane insertion. Atrial natriuretic factor, which signals via cGMP, also stimulated AQP2 translocation. AQP2 expression in kidney connecting tubules is sufficient for survival and that AQP2 expression in collecting ducts is required to regulate body water balance. The S256L substitution in the cytoplasmic tail of the Aqp2 protein prevented phosphorylation at S256 and the subsequent accumulation of Aqp2 on the apical membrane of the collecting duct principal cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat, Human, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Validation Images & Assay Conditions
Click image to see more details
Anti-Aquaporin 2 antibody, PA1742, IHC(P)
IHC(P): Rat Kidney Tissue
Click image to see more details
Anti-Aquaporin 2 antibody, PA1742, Western blotting
Lane 1: MCF-7 Cell Lysate
Lane 2: SW620 Cell Lysate
Lane 3: HT1080 Cell Lysate
Click image to see more details
Anti-Aquaporin 2 antibody, PA1742, IHC(F)
IHC(F): Rat Kidney Tissue
Protein Target Info & Infographic
Gene/Protein Information For AQP2 (Source: Uniprot.org, NCBI)
Gene Name
AQP2
Full Name
Aquaporin-2
Weight
28.837kDa
Superfamily
MIP/aquaporin (TC 1.A.8) family
Alternative Names
ADH water channel; AQP-2; AQP-CD; aquaporin 2 (collecting duct); aquaporin-2; aquaporin-CD; Collecting duct water channel protein; MGC34501; Water channel protein for renal collecting duct; water-channel aquaporin 2; WCH-CD AQP2 AQP-CD, WCH-CD aquaporin 2 aquaporin-2|ADH water channel|AQP-2|aquaporin 2 (collecting duct)|aquaporin-CD|collecting duct water channel protein|water channel protein for renal collecting duct|water-channel aquaporin 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AQP2, check out the AQP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AQP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Aquaporin 2/AQP2 Antibody (PA1742)
Hello CJ!
PA1742 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Expression pattern of aquaporins in patients with primary nephrotic syndrome with edema
Cui Wy, Tian Ay, Bai T. Clin Exp Pharmacol Physiol. 2011 Nov;38(11):747-54. Doi: 10.1111/J.1440-1681.2011.05584.X. Protective Effects Of Propofol On Endotoxemia-Induced Acute Kidney Injury In Rats.
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Anti-Aquaporin 2/AQP2 Antibody
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney using anti-Aquaporin 2/AQP2 antibody PA1742. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-28
Question
I see that the anti-Aquaporin 2/AQP2 antibody PA1742 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-10
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-10
Question
We are interested in using your anti-Aquaporin 2/AQP2 antibody for diabetes insipidus studies. Has this antibody been tested with western blotting on sw620 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
Thank you for your inquiry. This PA1742 anti-Aquaporin 2/AQP2 antibody is tested on rat kidney tissue, sw620 cell lysate, ht1080 cell lysate. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-12-04
Question
For PA1742, do you do permeabilization in your cell staining?
Verified customer
Asked: 2019-11-05
Answer
No, it is unnecessary to do permeabilization in the Anti-Aquaporin 2/AQP2 Antibody (PA1742) if the protein is a transmembrane protein.
Boster Scientific Support
Answered: 2019-11-06
Question
I was wanting to use to test anti-Aquaporin 2/AQP2 antibody PA1742 on human kidney for research purposes, then I may be interested in using anti-Aquaporin 2/AQP2 antibody PA1742 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
The products we sell, including anti-Aquaporin 2/AQP2 antibody PA1742, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-18
Question
Does PA1742 anti-Aquaporin 2/AQP2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
G. Dhar
Verified customer
Asked: 2019-08-19
Answer
You can see on the product datasheet, PA1742 anti-Aquaporin 2/AQP2 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-19
Question
Our team were content with the WB result of your anti-Aquaporin 2/AQP2 antibody. However we have been able to see positive staining in kidney apical cell membrane using this antibody. Is that expected? Could you tell me where is AQP2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-07-15
Answer
Based on literature, kidney does express AQP2. Generally AQP2 expresses in apical cell membrane. Regarding which tissues have AQP2 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Kidney, Pubmed ID: 7510718, 11929850
Boster Scientific Support
Answered: 2019-07-15
Question
We have tried in the past anti-Aquaporin 2/AQP2 antibody for IHC on colon a few years ago. I am using mouse, and We want to use the antibody for WB next. We need examining colon as well as cortex of kidney in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-06-25
Answer
I viewed the website and datasheets of our anti-Aquaporin 2/AQP2 antibody and it seems that PA1742 has been validated on mouse in both IHC and WB. Thus PA1742 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-06-25
Question
Is a blocking peptide available for product anti-Aquaporin 2/AQP2 antibody (PA1742)?
Verified Customer
Verified customer
Asked: 2019-02-18
Answer
We do provide the blocking peptide for product anti-Aquaporin 2/AQP2 antibody (PA1742). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-02-18
Question
I was wanting to use your anti-Aquaporin 2/AQP2 antibody for IHC for human kidney on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human kidney identification?
Verified Customer
Verified customer
Asked: 2018-12-11
Answer
You can see on the product datasheet, PA1742 anti-Aquaporin 2/AQP2 antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-12-11
Question
See attached the WB image, lot number and protocol we used for kidney using anti-Aquaporin 2/AQP2 antibody PA1742. Please let me know if you require anything else.
V. Krishna
Verified customer
Asked: 2018-01-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-12
Question
Is this PA1742 anti-Aquaporin 2/AQP2 antibody reactive to the isotypes of AQP2?
Verified Customer
Verified customer
Asked: 2017-09-19
Answer
The immunogen of PA1742 anti-Aquaporin 2/AQP2 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 2(245-260aa DWEEREVRRRQSVELH), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-09-19
Question
We are currently using anti-Aquaporin 2/AQP2 antibody PA1742 for mouse tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2017-06-08
Answer
The anti-Aquaporin 2/AQP2 antibody (PA1742) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-08
Question
Is there a BSA free version of anti-Aquaporin 2/AQP2 antibody PA1742 available?
N. Huang
Verified customer
Asked: 2017-05-18
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Aquaporin 2/AQP2 antibody PA1742 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-05-18
Question
I have a question about product PA1742, anti-Aquaporin 2/AQP2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
P. Walker
Verified customer
Asked: 2016-11-03
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1742 anti-Aquaporin 2/AQP2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-11-03
Question
We have been able to see staining in mouse kidney. Are there any suggestions? Is anti-Aquaporin 2/AQP2 antibody supposed to stain kidney positively?
G. Lewis
Verified customer
Asked: 2016-08-17
Answer
From what I have seen in literature kidney does express AQP2. From what I have seen in Uniprot.org, AQP2 is expressed in cortex of kidney, kidney, colon, among other tissues. Regarding which tissues have AQP2 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Kidney, Pubmed ID: 7510718, 11929850
Boster Scientific Support
Answered: 2016-08-17
Question
Will anti-Aquaporin 2/AQP2 antibody PA1742 work for IHC with kidney?
K. Baker
Verified customer
Asked: 2013-01-31
Answer
According to the expression profile of kidney, AQP2 is highly expressed in kidney. So, it is likely that anti-Aquaporin 2/AQP2 antibody PA1742 will work for IHC with kidney.
Boster Scientific Support
Answered: 2013-01-31