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- Table of Contents
AQP4 antibody (A01049) performs well in ELISA, Flow Cytometry, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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Flow Cytometry analysis of A431 cells using anti-Aquaporin 4/AQP4 antibody (A01049).
Overlay histogram showing A431 cells stained with A01049 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Aquaporin 4/AQP4 Antibody (A01049, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing A431 cells stained with A01049 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Aquaporin 4/AQP4 Antibody (A01049, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Aquaporin 4/AQP4 Antibody Picoband®
AQP4 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IHC, WB |
Tested Applications
AQP4 antibody was positively detected by WB in:
human U-87MG whole cell, rat brain tissue, mouse brain tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
AQP4 antibody was positively detected by IHC in:
mouse brain tissue, rat brain tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
AQP4 antibody was positively detected by FC in:
A431 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Mouse, Rat
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Recommend
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Aquaporin 4/AQP4 Antibody Picoband®
View all AQP4 Antibodies
View all AQP4 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Aquaporin 4/AQP4 recombinant protein (Position: L247-V323).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
32 kDa
Calculated Molecular Weight
34.8 kDa
Background of AQP4
Aquaporin 4 is found in the basolateral cell membrane of principal collecting duct cells and provides a pathway for water to exit these cells. The gene of AQP4 is mapped to 18q11.2-q12.1. Similar to other aquaporins, the AQP4 gene is composed of 4 exons encoding 127, 55, 27, and 92 amino acids separated by introns of 0.8, 0.3, and 5.2 kb. Unlike other aquaporins, an alternative coding initiation sequence (designated exon 0) was located 2.7 kb upstream of exon 1. When spliced together, M1 and the subsequent 10 amino acids are encoded by exon 0; the next 11 amino acids and M23 are encoded by exon 1. AQP4 is expressed in astrocytes and is upregulated by direct insult to the central nervous system. And AQP4 is the predominant water channel in the brain and has an important role in brain water homeostasis. It is abundant in mammalian brain and is concentrated in astrocytic foot processes at the blood-brain barrier.
Cite This Product
Anti-Aquaporin 4/AQP4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01049)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
97%
High Homology
Cow
96%
High Homology
Sheep
96%
High Homology
Mouse
94%
High Homology
Rabbit
94%
High Homology
Rat
92%
High Homology
Dog
91%
High Homology
Zebrafish
38%
Low homology
Drosophila
13%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for AQP4 antibody
Immunohistochemistry (IHC) protocol for AQP4 antibody
Flow Cytometry (FC) protocol for AQP4 antibody
Publications
Application:
Species:
Down-Regulation of AQP4 Expression via p38 MAPK Signaling in Temozolomide-Induced Glioma Cells Growth Inhibition and Invasion Impairment
Published: 2017-06-01
Journal: JOURNAL OF CELLULAR BIOCHEMISTRY
Application: WB
Impact Factor: 3.09
Species: Human
PMID: 28569417
Neuroprotective Effects of Total Steroid Saponins on Cerebral Ischemia Injuries in an Animal Model of Focal Ischemia/Reperfusion
Published: 2014-06-25
Journal: PLANTA MEDICA
Application: WB
Impact Factor: 2.34
Species: Rat
PMID: 24963614
Ulinastatin attenuates cerebral ischemia-reperfusion injury in rats
Published: 2014-05-15
Journal: International Journal of Clinical and Experimental Medicine
Application: WB
Impact Factor: 1.42
Species: Rat
PMID: 24995117
Review
Have you used Anti-Aquaporin 4/AQP4 Antibody Picoband®?
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1 Review For Anti-Aquaporin 4/AQP4 Antibody Picoband®
This antibody is highly efficient and specific, suitable for detecting AQP4 protein in rat colon by Western blot, with only minor nonspecific bands.
| SKU | A01049 |
|---|---|
| Application | Western Blot |
| Sample | rat colon tissue |
| Sample Processing Description | Samples were lysed in RIPA buffer containing protease inhibitor PMSF (100:1) for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE. |
| Other Reagents | Blocking buffer |
| Primary Antibody | Aquaporin 4/AQP4 Antibody Picoband® |
| Primary Incubation | 1:1000, overnight at 4 ℃ |
| Secondary Antibody | HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) |
| Secondary Incubation | 1 hour in room temperature |
| Detection | Substrate: ECL, Imaging system:ChemiDoc MP |
| Results Summary | The figure shows a schematic representation of Western blot results for the target protein AQP4 and the loading control Actin in rat colon across the normal, model, traditional Chinese medicine, and western medicine groups. The target bands are clear and distinct, and the experimental results are satisfactory. |
Shiyu Zhang, LUTCM
Verified customer
Submitted 2025-12-30
Product Promise
We promise Anti-Aquaporin 4/AQP4 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A01049
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 21, 2026