Click image to see more details
Product Info Summary
| SKU: | PB9753 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Arc Antibody Picoband®
SKU/Catalog Number
PB9753
PB0800 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Arc Antibody Picoband® catalog # PB9753. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Arc Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9753)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Arc, different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9753 is reactive to ARC in Human, Mouse, Rat
Observed Molecular Weight
45 kDa
Calculated molecular weight
45.3 kDa
Background of ARC
ARC, officially called activity-regulated cytoskeleton-associated protein, is a plasticity protein first characterized in 1995. It is a member of the immediate-early gene (IEG) family. The ARC gene is mapped to chromosome 8q24. It has got 460 amino acid protein which shares significant similarity with rat Arc. The Arc is highly expressed in heart, brain, lung, skeletal muscle, pancreas, prostate and testis and has got weak expression in small intestine, colon, and peripheral blood leukocytes. Arc is widely considered to be an important protein in neurobiology and also a significant tool for systems neuroscience.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9753 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human U20S whole cell,human 293T whole cell,human SH-SY5Y whole cell,human SiHa whole cell,rat brain tissue,rat C6 whole cell,mouse brain tissue,mouse Neuro-2a whole cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Arc using anti-Arc antibody (PB9753).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U20S whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human SH-SY5Y whole cell lysates,
Lane 4: human SiHa whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Arc antigen affinity purified polyclonal antibody (Catalog # PB9753) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Arc at approximately 45 kDa. The expected band size for Arc is at 45 kDa.
Specific Publications For Anti-Arc Antibody Picoband® (PB9753)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Arc Antibody Picoband®?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Arc Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
4 Customer Q&As for Anti-Arc Antibody Picoband®
Question
We are currently using anti-Arc antibody PB9753 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
The anti-Arc antibody (PB9753) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-27
Question
We have observed staining in rat embl:baa19667.1}. What should we do? Is anti-Arc antibody supposed to stain embl:baa19667.1} positively?
Verified Customer
Verified customer
Asked: 2018-10-05
Answer
Based on literature embl:baa19667.1} does express ARC. Based on Uniprot.org, ARC is expressed in hypothalamus, forebrain eco:0000312, embl:aaf07185.1, brain eco:0000312, embl:baa19667.1, embl:aah75802.1}
rc uterus eco:0000312, embl:aah12321.1, among other tissues. Regarding which tissues have ARC expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9039502
Brain, and Uterus, Pubmed ID: 15489334
Forebrain, Pubmed ID: 10970730
Boster Scientific Support
Answered: 2018-10-05
Question
I am interested in using your anti-Arc antibody for vesicle-mediated intercellular transport studies. Has this antibody been tested with western blotting on hela whole cell lysate? We would like to see some validation images before ordering.
D. Jackson
Verified customer
Asked: 2017-08-25
Answer
I appreciate your inquiry. This PB9753 anti-Arc antibody is tested on rat brain tissue, tissue lysate, testis tissue, mouse brain, panc whole cell lysate, hela whole cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-08-25
Question
My team were happy with the WB result of your anti-Arc antibody. However we have seen positive staining in forebrain {eco:0000312 extracellular vesicle membrane using this antibody. Is that expected? Could you tell me where is ARC supposed to be expressed?
A. Li
Verified customer
Asked: 2013-12-09
Answer
Based on literature, forebrain eco:0000312 does express ARC. Generally ARC expresses in extracellular vesicle membrane. Regarding which tissues have ARC expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9039502
Brain, and Uterus, Pubmed ID: 15489334
Forebrain, Pubmed ID: 10970730
Boster Scientific Support
Answered: 2013-12-09
