Anti-Arc Antibody Picoband™

ARC/ARG3.1 antibody

Boster Bio Anti-Arc Antibody Picoband™ catalog # PB9753. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PB9753
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-Arc Antibody Picoband™

View all ARC/ARG3.1 Antibodies

SKU/Catalog Number

PB9753

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Arc Antibody Picoband™ catalog # PB9753. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Arc Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9753)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Arc, different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB9753 is reactive to Arc in Human, Mouse, Rat

Applications

PB9753 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

45 kDa

Calculated molecular weight

45.321kDa

Background of ARC/ARG3.1

ARC, officially called activity-regulated cytoskeleton-associated protein, is a plasticity protein first characterized in 1995. It is a member of the immediate-early gene (IEG) family. The ARC gene is mapped to chromosome 8q24. It has got 460 amino acid protein which shares significant similarity with rat Arc. The Arc is highly expressed in heart, brain, lung, skeletal muscle, pancreas, prostate and testis and has got weak expression in small intestine, colon, and peripheral blood leukocytes. Arc is widely considered to be an important protein in neurobiology and also a significant tool for systems neuroscience.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For Arc (Source: Uniprot.org, NCBI)

Gene Name

Arc

Full Name

Activity-regulated cytoskeleton-associated protein

Weight

45.321kDa

Superfamily

ARC/ARG3.1 family

Alternative Names

activity-regulated cytoskeleton-associated protein; ARC/ARG3.1; KIAA0278Arg3.1Activity-regulated gene 3.1 protein homolog Arc|Arc33.1, C86064, arg3.1, mArc, Arc|activity regulated cytoskeletal-associated protein|activity-regulated cytoskeleton-associated protein|ARC/ARG3.1|activity-regulated gene 3.1 protein homolog

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Arc, check out the Arc Infographic

Arc infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Arc: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-Arc Antibody Picoband™

Question

We are currently using anti-Arc antibody PB9753 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2019-12-27

Answer

The anti-Arc antibody (PB9753) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-12-27

Question

We have observed staining in rat embl:baa19667.1}. What should we do? Is anti-Arc antibody supposed to stain embl:baa19667.1} positively?

Verified Customer

Verified customer

Asked: 2018-10-05

Answer

Based on literature embl:baa19667.1} does express ARC. Based on Uniprot.org, ARC is expressed in hypothalamus, forebrain eco:0000312, embl:aaf07185.1, brain eco:0000312, embl:baa19667.1, embl:aah75802.1} rc uterus eco:0000312, embl:aah12321.1, among other tissues. Regarding which tissues have ARC expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9039502
Brain, and Uterus, Pubmed ID: 15489334
Forebrain, Pubmed ID: 10970730

Boster Scientific Support

Answered: 2018-10-05

Question

I am interested in using your anti-Arc antibody for vesicle-mediated intercellular transport studies. Has this antibody been tested with western blotting on hela whole cell lysate? We would like to see some validation images before ordering.

D. Jackson

Verified customer

Asked: 2017-08-25

Answer

I appreciate your inquiry. This PB9753 anti-Arc antibody is tested on rat brain tissue, tissue lysate, testis tissue, mouse brain, panc whole cell lysate, hela whole cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2017-08-25

Question

My team were happy with the WB result of your anti-Arc antibody. However we have seen positive staining in forebrain {eco:0000312 extracellular vesicle membrane using this antibody. Is that expected? Could you tell me where is ARC supposed to be expressed?

A. Li

Verified customer

Asked: 2013-12-09

Answer

Based on literature, forebrain eco:0000312 does express ARC. Generally ARC expresses in extracellular vesicle membrane. Regarding which tissues have ARC expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9039502
Brain, and Uterus, Pubmed ID: 15489334
Forebrain, Pubmed ID: 10970730

Boster Scientific Support

Answered: 2013-12-09

Order DetailsPrice
PB9753

100μg

$370
PB9753-10ug

10μg sample (liquid)

$99
PB9753-Biotin

100 μg Biotin conjugated

$570
PB9753-Cy3

100 μg Cy3 conjugated

$570
PB9753-Dylight488

100 μg Dylight488 conjugated

$570
PB9753-Dylight550

100 μg Dylight550 conjugated

$570
PB9753-Dylight594

100 μg Dylight594 conjugated

$570
PB9753-FITC

100 μg FITC conjugated

$570
PB9753-HRP

100 μg HRP conjugated

$570
PB9753-APC

100 μg APC conjugated

$670
PB9753-PE

100 μg PE conjugated

$670
PB9753-carrier-free

Carrier Free

$370

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PB9753
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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