Product Info Summary
SKU: | PB9753 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Arc Antibody Picoband™
View all ARC/ARG3.1 Antibodies
SKU/Catalog Number
PB9753
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Arc Antibody Picoband™ catalog # PB9753. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Arc Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9753)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Arc, different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9753 is reactive to Arc in Human, Mouse, Rat
Applications
PB9753 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
45 kDa
Calculated molecular weight
45.321kDa
Background of ARC/ARG3.1
ARC, officially called activity-regulated cytoskeleton-associated protein, is a plasticity protein first characterized in 1995. It is a member of the immediate-early gene (IEG) family. The ARC gene is mapped to chromosome 8q24. It has got 460 amino acid protein which shares significant similarity with rat Arc. The Arc is highly expressed in heart, brain, lung, skeletal muscle, pancreas, prostate and testis and has got weak expression in small intestine, colon, and peripheral blood leukocytes. Arc is widely considered to be an important protein in neurobiology and also a significant tool for systems neuroscience.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Arc using anti-Arc antibody (PB9753).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Brain Tissue Lysate at 50ug,
Lane 2: Rat Testis Tissue Lysate at 50ug,
Lane 3: Mouse Brain Tissue Lysate at 50ug,
Lane 4: PANC Whole Cell Lysate at 40ug,
Lane 5: HELA Whole Cell Lysate at 40ug,
Lane 6: MCF-7 Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Arc antigen affinity purified polyclonal antibody (Catalog # PB9753) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Arc at approximately 45 kDa. The expected band size for Arc is at 45 kDa.
Protein Target Info & Infographic
Gene/Protein Information For Arc (Source: Uniprot.org, NCBI)
Gene Name
Arc
Full Name
Activity-regulated cytoskeleton-associated protein
Weight
45.321kDa
Superfamily
ARC/ARG3.1 family
Alternative Names
activity-regulated cytoskeleton-associated protein; ARC/ARG3.1; KIAA0278Arg3.1Activity-regulated gene 3.1 protein homolog Arc|Arc33.1, C86064, arg3.1, mArc, Arc|activity regulated cytoskeletal-associated protein|activity-regulated cytoskeleton-associated protein|ARC/ARG3.1|activity-regulated gene 3.1 protein homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Arc, check out the Arc Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Arc: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Arc Antibody Picoband™ (PB9753)
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No publications found for PB9753
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Arc Antibody Picoband™
Question
We are currently using anti-Arc antibody PB9753 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
The anti-Arc antibody (PB9753) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-27
Question
We have observed staining in rat embl:baa19667.1}. What should we do? Is anti-Arc antibody supposed to stain embl:baa19667.1} positively?
Verified Customer
Verified customer
Asked: 2018-10-05
Answer
Based on literature embl:baa19667.1} does express ARC. Based on Uniprot.org, ARC is expressed in hypothalamus, forebrain eco:0000312, embl:aaf07185.1, brain eco:0000312, embl:baa19667.1, embl:aah75802.1}
rc uterus eco:0000312, embl:aah12321.1, among other tissues. Regarding which tissues have ARC expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9039502
Brain, and Uterus, Pubmed ID: 15489334
Forebrain, Pubmed ID: 10970730
Boster Scientific Support
Answered: 2018-10-05
Question
I am interested in using your anti-Arc antibody for vesicle-mediated intercellular transport studies. Has this antibody been tested with western blotting on hela whole cell lysate? We would like to see some validation images before ordering.
D. Jackson
Verified customer
Asked: 2017-08-25
Answer
I appreciate your inquiry. This PB9753 anti-Arc antibody is tested on rat brain tissue, tissue lysate, testis tissue, mouse brain, panc whole cell lysate, hela whole cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-08-25
Question
My team were happy with the WB result of your anti-Arc antibody. However we have seen positive staining in forebrain {eco:0000312 extracellular vesicle membrane using this antibody. Is that expected? Could you tell me where is ARC supposed to be expressed?
A. Li
Verified customer
Asked: 2013-12-09
Answer
Based on literature, forebrain eco:0000312 does express ARC. Generally ARC expresses in extracellular vesicle membrane. Regarding which tissues have ARC expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9039502
Brain, and Uterus, Pubmed ID: 15489334
Forebrain, Pubmed ID: 10970730
Boster Scientific Support
Answered: 2013-12-09