This website uses cookies to ensure you get the best experience on our website.
Mouse BDNF ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse BDNF. 96wells/kit, with removable strips. Cited in 32 publication(s). Independently reviewed in 1 review(s).
Reviews (1) See details
Mouse BDNF ELISA Kit PicoKine™ Info At A Glance
| SKU: | EK0309 |
|---|---|
| Size: | 96wells/kit, with removable strips. |
| Reactivity: | Mouse |
| Sample Type: | cell culture supernates, cell lysates, serum and plasma (heparin, EDTA, citrate). |
| Sample Volume: | 100μl per well |
| Sensitivity: | <15pg/ml |
| Assay Range: | 31.2pg/ml-2000pg/ml |
Overview
| Product Name | Mouse BDNF ELISA Kit PicoKine™
See all Bdnf primary antibodies, ELISA kits and proteins |
|---|---|
| SKU/Catalog Number | EK0309 |
| Storage & Handling | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description | Detect Mouse BDNF with <2pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. BDNF tissue specificity: |
| Cite This Product | Mouse BDNF ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0309) |
Product Specs
| Reactivity/Species | Mouse |
|---|---|
| Applications | ELISA
*Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab. |
| Sample Type | cell culture supernates, cell lysates, serum and plasma (heparin, EDTA, citrate). |
| Sensitivity | <15pg/ml |
| Assay Range | 31.2pg/ml-2000pg/ml |
| Immunogen | Expression system for standard: sf21; Immunogen sequence: H129-R247 |
| Antibody Clonalities | Capture antibody|Detection antibody monoclonal antibody from rat|polyclonal antibody from goat |
| Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size | 96wells/kit, with removable strips. |
Protein Target Info (Source: Uniprot.org)
| Uniprot Id | P21237 |
|---|---|
| Gene Name | Bdnf |
| Protein Name | Brain-derived neurotrophic factor |
| Subcellular Localization | Secreted. |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
| Protein Function | During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. |
|---|---|
| Background | Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Functions as a hormone. |
Kit Components And Assay QC Data Details Of Mouse BDNF ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
|---|---|---|
| EK0309-CAP | 96-well plate precoated with anti-Mouse Bdnf antibody | 1 |
| EK0309-ST | lyophilized recombinant Mouse Bdnf standard | 10ng/tube |
| EK0309-DA | biotinylated anti-Mouse Bdnf antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0309 Mouse BDNF ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.037 | 0.077 | 0.095 | 0.164 | 0.358 | 0.846 | 1.450 | 2.237 |
We measured random samples of Mouse BDNF ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 58 | 196 | 923 | 61 | 198 | 920 |
| Standard deviation | 4.06 | 14.5 | 46.15 | 5.42 | 18.21 | 60.72 |
| CV(%) | 7% | 7.4% | 5% | 8.9% | 9.2% | 6.6% |
We measured the performance consistency of Mouse BDNF ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 58 | 59 | 58 | 68 | 60 | 4.2 | 7% |
| Sample 2 | 196 | 221 | 215 | 235 | 216 | 14 | 6.4% |
| Sample 3 | 923 | 885 | 975 | 808 | 897 | 60.87 | 6.7% |
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 32
| Tajerian M, Leu D, Zou Y, Sahbaie P, Li W, Khan H, Hsu V, Kingery W, Huang Tt, Becerra L, Clark Jd. Anesthesiology. 2014 Oct;121(4):852-65. Doi: 10.1097/Aln.0000000000000403. Brain Neuroplastic Changes Accompany Anxiety And Memory Deficits In A Mo... | PubMed ID 25093591 |
| Chen, G., Ma, Q., Goswami, D., Shang, J., & Miller, G.M. (2017). Modulation of nuclear REST by alternative splicing: a potential therapeutic target for Huntington's disease. Journal of Cellular and Molecular Medicine. Advance online publication. d... | PubMed ID 28524599 |
| Schulze, J., Kaiser, O., Paasche, G., Lamm, H., Pich, A., Hoffmann, A.,..., & Warnecke, A. (2017). Effect of hyperbaric oxygen on BDNF-release and neuroprotection: Investigations with human mesenchymal stem cells and genetically modified NIH3T3 fi... | PubMed ID 28542481 |
| Zhang, Q., Wang, X., Bai, X., Xie, Y., Zhang, T., Bo, S., & Chen, X. (2017). Resveratrol reversed chronic restraint stress-induced impaired cognitive function in rats. Molecular Medicine Reports, 16(2), 2095-2100. Advance online publication. doi: ... | PubMed ID 28656303 |
| Koh EJ, Kim KJ, Song JH, Choi J, Lee HY, Kang DH, Heo HJ, Lee BY. Int J Mol Sci. 2017 Nov 13;18(11). pii: E2401. doi: 10.3390/ijms18112401. Spirulina maxima Extract Ameliorates Learning and Memory Impairments via Inhibiting GSK-3β Phosphorylation ... | PubMed ID 29137190 |
| Li M, Li C, Yu H, Cai X, Shen X, Sun X, Wang J, Zhang Y, Wang C. J Neuroinflammation. 2017 Sep 20;14(1):190. doi: 10.1186/s12974-017-0964-9. Lentivirus-mediated interleukin-1β (IL-1β) knock-down in the hippocampus alleviates lipopolysaccharide (LP... | PubMed ID 28931410 |
| Phosphodiesterase Type 4 Inhibitor Rolipram Improves Survival of Spiral Ganglion Neurons?In Vitro | PubMed ID 24642701 |
| The effects of EGb761 on lipopolysaccharide-induced depressive-like behaviour in C57BL/6J mice | PubMed ID 26155178 |
| Differential Efficacy of Ketamine in the Acute versus Chronic Stages of Complex Regional Pain Syndrome in Mice. | PubMed ID 26492479 |
| Fingolimod induces neurogenesis in adult mouse hippocampus and improves contextual fear memory | PubMed ID 26795749 |
| Granulocyte colony-stimulating factor promotes behavioral recovery in a mouse model of traumatic brain injury | PubMed ID 26822127 |
| Magnetic Beads Enhance Adhesion of NIH 3T3 Fibroblasts: A Proof-of-Principle In Vitro Study for Implant-Mediated Long-Term Drug Delivery to the Inner Ear | PubMed ID 26918945 |
| The behavioral and physiological effects of high?fat diet and alcohol consumption: Sex differences in C57BL6/J mice | PubMed ID 28638713 |
| Ondansetron ameliorates depression associated with obesity in high-fat diet fed experimental mice: An investigation-based on the behavioral, biochemical, and molecular approach | PubMed ID 29326489 |
| Chronic Hippocampal Abnormalities and Blunted HPA Axis in an Animal Model of Repeated Unpredictable Stress | PubMed ID 30079015 |
| Neurotherapeutic Effect of Cord Blood Derived CD45 Hematopoietic Cells in Mice after Traumatic Brain Injury | PubMed ID 29274773 |
| Neuroprotective role of Indirubin-3'-monoxime, a GSKβ inhibitor in high fat diet induced cognitive impairment in mice. | PubMed ID 25234596 |
| Impact of Repetitive Mild Traumatic Brain Injury on Behavioral and Hippocampal Deficits in a Mouse Model of Chronic Stress | PubMed ID 30963958 |
| Male C57BL6/N and C57BL6/J Mice Respond Differently to Constant Light and Running-Wheel Access. | PubMed ID 31920578 |
| Antidepressant-like effect of macranthol isolated from Illicium dunnianum tutch in mice. | PubMed ID 23524090 |
| Therapeutic potential of silymarin in chronic unpredictable mild stress induced depressive-like behavior in mice. | PubMed ID 29215318 |
| Etazolate rescues behavioral deficits in chronic unpredictable mild stress model: modulation of hypothalamic-pituitary-adrenal axis activity and brain-derived neurotrophic factor level. | PubMed ID 23974048 |
| BDNF signaling is necessary for the antidepressant-like effect of naringenin. | PubMed ID 24121063 |
| Sexual dimorphism in BDNF signaling after neonatal hypoxia-ischemia and treatment with necrostatin-1 | PubMed ID 24361177 |
| Physical exercise induces structural alterations in the hippocampal astrocytes: exploring the role of BDNF-TrkB signaling | PubMed ID 27686571 |
| Transduced PTD-BDNF fusion protein protects against beta amyloid peptide-induced learning and memory deficits in mice | PubMed ID 18191117 |
| Circadian variations in behaviors, BDNF and cell proliferation in depressive mice | PubMed ID 26183613 |
| Essential oil of Perilla frutescens-induced change in hippocampal expression of brain-derived neurotrophic factor in chronic unpredictable mild stress in mice | PubMed ID 23506995 |
| Histone deacetylase inhibitor, trichostatin A, improves learning and memory in high-fat diet-induced cognitive deficits in mice | PubMed ID 25391764 |
| Effect of mild hypothermia on angiogenesis in rats with focal cerebral ischemia | PubMed ID 17630209 |
| Epigenetic modifications by inhibiting histone deacetylases reverse memory impairment in insulin resistance induced cognitive deficit in mice | PubMed ID 26805421 |
| Apigenin reverses depression-like behavior induced by chronic corticosterone treatment in mice | PubMed ID 26826594 |
Submit your review to [email protected] or to Biocompare.com for an Amazon giftcard.
1 reviews
"It is a good kit with good quality."
Source: Biocompare.com
"It is a good kit with good quality."
--JUNHUI WANG UNIVERSITY OF TORONTO PHYSIOLOGY RESEARCH ASSOCIATE
| Applications | ELISA |
|---|---|
| Sample | Brain Tissue Lysate |
| Detection | -- |
"I used the ELISA kit to detect the BDNF level in several samples, including brain tissues (cortex and hippocampus), cultured astrocyte (cell lysis) and astrocyte conditioned medium. The kit works very well for cell lysis and brain tissues according to the instruction. For the cell medium, I suggest that users need modify the protocol a little bit, such as prolong the incubation time for samples in the wells for overnight. The kit has a good quality."
Contact us with any questions at [email protected] or go to contact us
Questions and answers from customers related to EK0309 Mouse BDNF ELISA Kit PicoKine™
9 Related Questions
Question
Q: Does EK0309 measure all BDNF isoforms (pro and mature BDNF)? Keywords: isoform detection, pro BDNF, mature BDNF
Verified Customer
Asked: 0000-00-00
Answer
A: EK0309 can measure both pro and mature BDNF. However, EK0309 cannot differentiate between pro BDNF and mature BDNF.
Boster Scientific Support
Answered: 0000-00-00
Question
Q: When choosing between lysis buffer and PBS to prepare tissue lysates, is there any benefit to using one over the other? Keywords: lysis buffer, TBS, PBS, washing buffer, lysate preparation, RIPA, ELISA
Verified Customer
Asked: 0000-00-00
Answer
A: RIPA lysis buffer is the preferred buffer for lysate preparation because it lyses tissues more thoroughly than PBS.
Boster Scientific Support
Answered: 0000-00-00
Question
Q: Will using RIPA lysis system for lysate preparation have an effect on the ELISA Keywords: lysis buffer, TBS, PBS, washing buffer, lysate preparation, RIPA, ELISA
Verified Customer
Asked: 0000-00-00
Answer
A: Using RIPA lysis system will not have any effect on the ELISA.
Boster Scientific Support
Answered: 0000-00-00
Question
Q: I got very low OD values for the BDNF standard that you provided. What should I do?
Verified Customer
Asked: 0000-00-00
Answer
A: Try to repeat your experiment by extending your incubation times as follows: samples (150 min), biotinylated antibodies (70-80 min), ABC reagent (45 min) and TMB (30 min).
Boster Scientific Support
Answered: 0000-00-00
Question
Q: I am interested in performing Western Blot (WB) using the antibodies from your human and mouse BDNF ELISA kits. Is it possible to order the primary antibody (the capture antibody) that is pre-coated in the wells for the sandwich ELISA? (In this case that would be the monoclonal antibody from rat for the mouse kit, and the monoclonal antibody from mouse for the human kit). Is it also possible to order your secondary antibody which is the biotinylated detection polyclonal antibody from goat specific for BDNF?
Verified Customer
Asked: 0000-00-00
Answer
A: The antibodies used in those ELISA kits are not fully validated on WB. Also, since these antibodies are made from recombinant proteins, they are often less reactive to retrieved antigens in WB or IHC due to the lack of tertiary structure in the retrieved antigens. If you want to test human and mouse BDNF with WB, please use our anti-BDNF antibody (PB9075). Further, you can separately buy our secondary antibodies.
Boster Scientific Support
Answered: 0000-00-00
Question
Q: Could cell lysate also be tested by this kit? What is your advice on how to prepare cell lysates? Keyword: application, sample, protocol
Verified Customer
Asked: 0000-00-00
Answer
A: We didn't test cell lysate, the kit can work if the concentration of BDNF in cell lysate is in the test range of the kit. The customer can have a try if he wants to detect it. The general preparation of cell lysate is as follows: Collect cells and rinse cells with PBS. Homogenize and lyse cells thoroughly in lysate solution(Cell Protein Extraction Reagent, Catalog# AR0103). Centrifuge cell lysates at approximately 10000 X g for 5 min to remove debris. Aliquots of the cell lysates were removed and assayed.
Boster Scientific Support
Answered: 0000-00-00
Order Product (EK0309)
Shipping Information
Domestically in USA and Canada, ships next business day if available in stock.
Note: sample size takes longer to process, average 5-7 days.
Contact Information
| [email protected] | |
| (888) 466-3604 |
Boster Guarantee
All Boster antibodies and ELISA kits are guaranteed to meet the specifications on the data sheet.
See Details
In stock
Order Product
EK0309
$369.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.