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Product Info Summary
| SKU: | EK0309 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate). |
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Product info
Product Name
Mouse BDNF ELISA Kit PicoKine™
SKU/Catalog Number
EK0309
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse BDNF ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Bdnf in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate). Sensitivity: 15pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Mouse BDNF ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0309)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: H129-R247
Sensitivity
<15 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0309 is reactive to Bdnf in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK0309 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0309-CAP | Anti-Mouse Bdnf Pre-coated 96-well strip microplate | 1 |
| EK0309-ST | Mouse Bdnf Standard | 2 vials, 10 ng/tube |
| EK0309-DA | Mouse Bdnf Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.018 | 0.046 | 0.059 | 0.103 | 0.219 | 0.451 | 0.979 | 1.863 |
Data Example Images
Click image to see more details
Mouse BDNF PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse BDNF ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 58 | 196 | 923 | 61 | 198 | 920 |
| Standard deviation | 4.06 | 14.5 | 46.15 | 5.42 | 18.21 | 60.72 |
| CV (%) | 7% | 7.4% | 5% | 8.9% | 9.2% | 6.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Bdnf in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 58 | 59 | 58 | 68 | 60 | 4.2 | 7% |
| Sample 2 | 196 | 221 | 215 | 235 | 216 | 14 | 6.4% |
| Sample 3 | 923 | 885 | 975 | 808 | 897 | 60.87 | 6.7% |
Protein Target Info & Infographic
Gene/Protein Information For Bdnf (Source: Uniprot.Org, NCBI)
Uniprot ID
P21237
Gene ID
12064
Gene Name
Bdnf
Full Name
Brain-derived neurotrophic factor
Weight
28.123kDa
Superfamily
NGF-beta family
Alternative Names
Abrineurin; ANON2; BDNF; brain-derived neurotrophic factor; BULN2; MGC34632; Neurotrophin Bdnf|brain derived neurotrophic factor|brain-derived neurotrophic factor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Bdnf, check out the Bdnf Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Bdnf: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Mouse BDNF ELISA Kit PicoKine™ (EK0309)
EK0309 has been cited in 34 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Biney RP,Benneh CK,Adongo DW,Ameyaw EO,Woode E.Evidence of an antidepressant-like effect of xylopic acid mediated by serotonergic mechanisms.Psychopharmacology (Berl).2021 Apr 10.doi:10.1007/s00213-021-05835-6.Epub ahead of print.PMID:33837810.
Species: Mouse
EK0309 usage in article: APP:ELISA, SAMPLE:BRAIN TISSUE, DILUTION:NA
Thakare VN, Lakade SH, Mahajan MP, Kulkarni YP, Dhakane VD, Harde MT, Patel BM. Protocatechuic acid attenuates chronic unpredictable mild stress induced-behavioral and biochemical alterations in mice. Eur J Pharmacol. 2021 Mar 3;898:173992. doi: 10.1016/j.ejphar.2021.173992. Epub ahead of print. PMID: 33675783.
Species: Mouse
EK0309 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Apigenin reverses depression-like behavior induced by chronic corticosterone treatment in mice
Epigenetic modifications by inhibiting histone deacetylases reverse memory impairment in insulin resistance induced cognitive deficit in mice
Effect of mild hypothermia on angiogenesis in rats with focal cerebral ischemia
Histone deacetylase inhibitor, trichostatin A, improves learning and memory in high-fat diet-induced cognitive deficits in mice
Essential oil of Perilla frutescens-induced change in hippocampal expression of brain-derived neurotrophic factor in chronic unpredictable mild stress in mice
Circadian variations in behaviors, BDNF and cell proliferation in depressive mice
Transduced PTD-BDNF fusion protein protects against beta amyloid peptide-induced learning and memory deficits in mice
Physical exercise induces structural alterations in the hippocampal astrocytes: exploring the role of BDNF-TrkB signaling
Sexual dimorphism in BDNF signaling after neonatal hypoxia-ischemia and treatment with necrostatin-1
BDNF signaling is necessary for the antidepressant-like effect of naringenin.
Etazolate rescues behavioral deficits in chronic unpredictable mild stress model: modulation of hypothalamic-pituitary-adrenal axis activity and brain-derived neurotrophic factor level.
Therapeutic potential of silymarin in chronic unpredictable mild stress induced depressive-like behavior in mice.
Antidepressant-like effect of macranthol isolated from Illicium dunnianum tutch in mice.
Male C57BL6/N and C57BL6/J Mice Respond Differently to Constant Light and Running-Wheel Access.
Impact of Repetitive Mild Traumatic Brain Injury on Behavioral and Hippocampal Deficits in a Mouse Model of Chronic Stress
Neuroprotective role of Indirubin-3'-monoxime, a GSKβ inhibitor in high fat diet induced cognitive impairment in mice.
Neurotherapeutic Effect of Cord Blood Derived CD45 Hematopoietic Cells in Mice after Traumatic Brain Injury
Chronic Hippocampal Abnormalities and Blunted HPA Axis in an Animal Model of Repeated Unpredictable Stress
Ondansetron ameliorates depression associated with obesity in high-fat diet fed experimental mice: An investigation-based on the behavioral, biochemical, and molecular approach
The behavioral and physiological effects of high?fat diet and alcohol consumption: Sex differences in C57BL6/J mice
Magnetic Beads Enhance Adhesion of NIH 3T3 Fibroblasts: A Proof-of-Principle In Vitro Study for Implant-Mediated Long-Term Drug Delivery to the Inner Ear
Granulocyte colony-stimulating factor promotes behavioral recovery in a mouse model of traumatic brain injury
Fingolimod induces neurogenesis in adult mouse hippocampus and improves contextual fear memory
Differential Efficacy of Ketamine in the Acute versus Chronic Stages of Complex Regional Pain Syndrome in Mice.
The effects of EGb761 on lipopolysaccharide-induced depressive-like behaviour in C57BL/6J mice
Phosphodiesterase Type 4 Inhibitor Rolipram Improves Survival of Spiral Ganglion Neurons?In Vitro
Li M, Li C, Yu H, Cai X, Shen X, Sun X, Wang J, Zhang Y, Wang C. J Neuroinflammation. 2017 Sep 20;14(1):190. doi: 10.1186/s12974-017-0964-9. Lentivirus-mediated interleukin-1β (IL-1β) knock-down in the hippocampus alleviates lipopolysaccharide (LP...
Koh EJ, Kim KJ, Song JH, Choi J, Lee HY, Kang DH, Heo HJ, Lee BY. Int J Mol Sci. 2017 Nov 13;18(11). pii: E2401. doi: 10.3390/ijms18112401. Spirulina maxima Extract Ameliorates Learning and Memory Impairments via Inhibiting GSK-3β Phosphorylation ...
Zhang, Q., Wang, X., Bai, X., Xie, Y., Zhang, T., Bo, S., & Chen, X. (2017). Resveratrol reversed chronic restraint stress-induced impaired cognitive function in rats. Molecular Medicine Reports, 16(2), 2095-2100. Advance online publication. doi: ...
Schulze, J., Kaiser, O., Paasche, G., Lamm, H., Pich, A., Hoffmann, A.,..., & Warnecke, A. (2017). Effect of hyperbaric oxygen on BDNF-release and neuroprotection: Investigations with human mesenchymal stem cells and genetically modified NIH3T3 fi...
Chen, G., Ma, Q., Goswami, D., Shang, J., & Miller, G.M. (2017). Modulation of nuclear REST by alternative splicing: a potential therapeutic target for Huntington's disease. Journal of Cellular and Molecular Medicine. Advance online publication. d...
Tajerian M, Leu D, Zou Y, Sahbaie P, Li W, Khan H, Hsu V, Kingery W, Huang Tt, Becerra L, Clark Jd. Anesthesiology. 2014 Oct;121(4):852-65. Doi: 10.1097/Aln.0000000000000403. Brain Neuroplastic Changes Accompany Anxiety And Memory Deficits In A Mo...
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Customer Reviews
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1 Reviews For Mouse BDNF ELISA Kit PicoKine™
0
"It is a good kit with good quality."
Excellent
Source: Biocompare.com
"It is a good kit with good quality."
--JUNHUI WANG UNIVERSITY OF TORONTO PHYSIOLOGY RESEARCH ASSOCIATE
| Applications | ELISA |
|---|---|
| Sample | Brain Tissue Lysate |
| Detection | -- |
"I used the ELISA kit to detect the BDNF level in several samples, including brain tissues (cortex and hippocampus), cultured astrocyte (cell lysis) and astrocyte conditioned medium. The kit works very well for cell lysis and brain tissues according to the instruction. For the cell medium, I suggest that users need modify the protocol a little bit, such as prolong the incubation time for samples in the wells for overnight. The kit has a good quality."
Junhui Wang
Verified customer
Submitted 2019-05-24
Customer Q&As
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Find answers in Q&As, reviews.
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17 Customer Q&As for Mouse BDNF ELISA Kit PicoKine™
Question
Is there an alternate wash buffer recipe for EK0309?
Verified customer
Asked: 2021-02-17
Answer
For the Mouse BDNF ELISA Kit PicoKine™ (EK0309), please see the following wash buffer recipe: Add 1.2g Tris, 8.5g Nacl, 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L. You can also contact our orders department to ask for extra wash buffer.
Boster Scientific Support
Answered: 2021-02-18
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification Bdnf using the EK0309 Mouse Bdnf Picokine® ELISA Kit?
K. Wu
Verified customer
Asked: 2020-11-05
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2020-11-05
Question
Q: is it okay to use citrate plasma as samples in Mouse Bdnf Picokine® ELISA Kit (Catalog # EK0309)?
Verified Customer
Verified customer
Asked: 2020-05-31
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of Bdnf causing degradation of its protein structure. Bdnf may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Bdnf ELISA, treating samples with various anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-05-31
Question
Is the sf21 expression system referring to insect cells? Keywords: insect cell line, immunogen, IPLB-Sf21-AE
Verified Customer
Verified customer
Asked: 2020-04-20
Answer
Yes, sf21 is an insect cell expression system.
Boster Scientific Support
Answered: 2020-04-20
Question
When choosing between lysis buffer and PBS to prepare tissue lysates, is there any benefit to using one over the other? Keywords: lysis buffer, TBS, PBS, washing buffer, lysate preparation, RIPA, ELISA
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
RIPA lysis buffer is the preferred buffer for lysate preparation because it lyses tissues more thoroughly than PBS.
Boster Scientific Support
Answered: 2020-03-31
Question
Does EK0309 measure all BDNF isoforms (pro and mature BDNF)? Keywords: isoform detection, pro BDNF, mature BDNF
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
EK0309 can measure both pro and mature BDNF. However, EK0309 cannot differentiate between pro BDNF and mature BDNF.
Boster Scientific Support
Answered: 2019-09-19
Question
Will using RIPA lysis system for lysate preparation have an effect on the ELISA Keywords: lysis buffer, TBS, PBS, washing buffer, lysate preparation, RIPA, ELISA
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
Using RIPA lysis system will not have any effect on the ELISA.
Boster Scientific Support
Answered: 2019-09-18
Question
Is it normal to have bubbles in the wells or the plate of EK0309? Will it affect the performance of the kit?
Verified customer
Asked: 2019-08-08
Answer
The bubbles would not affect the result of the Mouse BDNF ELISA Kit PicoKine™ (EK0309), Please wash the plate to remove the bubbles as suggested below prior to the assay. Wash the plate 3 times with the 1x wash buffer. a. Discard the liquid in the wells into an appropriate waste receptacle. Then, invert the plate on the benchtop onto a paper towel and tap the plate to gently blot any remaining liquid. It is recommended that the wells are not allowed to completely dry at any time. b. Add 300 µl of the 1x wash buffer to each assay well. (For cleaner background incubate for 60 seconds between each wash). c. Repeat steps a-b 2 additional times.
Boster Scientific Support
Answered: 2019-08-08
Question
Could cell lysate also be tested by this kit? What is your advice on how to prepare cell lysates? Keyword: application, sample, protocol
Verified Customer
Verified customer
Asked: 2019-06-07
Answer
We didn't test cell lysate, the kit can work if the concentration of BDNF in cell lysate is in the test range of the kit. The customer can have a try if he wants to detect it. The general preparation of cell lysate is as follows: Collect cells and rinse cells with PBS. Homogenize and lyse cells thoroughly in lysate solution(Cell Protein Extraction Reagent, Catalog# AR0103). Centrifuge cell lysates at approximately 10000 X g for 5 min to remove debris. Aliquots of the cell lysates were removed and assayed.
Boster Scientific Support
Answered: 2019-06-07
Question
Q: how to analyze ELISA data? I have obtained Bdnf level in cell lysates.
K. Wu
Verified customer
Asked: 2019-05-05
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-05-05
Question
Q: if the enzyme conjugated Bdnf antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Bdnf antigen?
V. Kapoor
Verified customer
Asked: 2019-02-13
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-02-13
Question
Does this kit contain any recombinant protein produced by viral systems? Keyword: expression
Verified Customer
Verified customer
Asked: 2019-02-05
Answer
The kit does not contain protein produced by viral system, the express system of it is sf21.
Boster Scientific Support
Answered: 2019-02-05
Question
I am interested in performing Western Blot (WB) using the antibodies from your human and mouse BDNF ELISA kits. Is it possible to order the primary antibody (the capture antibody) that is pre-coated in the wells for the sandwich ELISA? (In this case that would be the monoclonal antibody from rat for the mouse kit, and the monoclonal antibody from mouse for the human kit). Is it also possible to order your secondary antibody which is the biotinylated detection polyclonal antibody from goat specific for BDNF?
Verified Customer
Verified customer
Asked: 2018-11-28
Answer
The antibodies used in those ELISA kits are not fully validated on WB. Also, since these antibodies are made from recombinant proteins, they are often less reactive to retrieved antigens in WB or IHC due to the lack of tertiary structure in the retrieved antigens. If you want to test human and mouse BDNF with WB, please use our anti-BDNF antibody (PB9075). Further, you can separately buy our secondary antibodies.
Boster Scientific Support
Answered: 2018-11-28
Question
Q: how to thaw whole blood sample for Bdnf ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-08-01
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Bdnf for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2018-08-01
Question
Is baculovirus used in the product of EK0309? Keyword: manufacturing
Verified Customer
Verified customer
Asked: 2018-05-29
Answer
Yes, baculovirus is used during the production of EK0309 as a transfection factor.
Boster Scientific Support
Answered: 2018-05-29
Question
Q: Can Bdnf ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2018-03-04
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-03-04
Question
I got very low OD values for the BDNF standard that you provided. What should I do?
Verified Customer
Verified customer
Asked: 2014-10-23
Answer
Try to repeat your experiment by extending your incubation times as follows: samples (150 min), biotinylated antibodies (70-80 min), ABC reagent (45 min) and TMB (30 min).
Boster Scientific Support
Answered: 2014-10-23
