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Product Info Summary
| SKU: | EK0309 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Mouse BDNF ELISA Kit PicoKine®
SKU/Catalog Number
EK0309
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse BDNF ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Bdnf in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate). Sensitivity: 15pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse BDNF ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0309)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: H129-R247
Sensitivity
<15 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0309 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0309 is reactive to BDNF in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK0309 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Bdnf
Brain-derived neurotrophic factor (BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor (NGF). BDNF and neurotrophin-3 (NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system. BDNF and other neurotrophins are critically involved in long-term potentiation (LTP). BDNF-mediated LTP is induced postsynaptically. BDNF has trophic effects on serotonergic (5-HT) neurons in the central nervous system. BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state. It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury. The mature form of BDNF is identical in all mammals examined, and the gene encoding human BDNF to chromosome 11, band p13.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0309-CAP | Anti-Mouse BDNF Pre-coated 96-well strip microplate | 1 |
| EK0309-ST | Mouse BDNF Standard | 2 vials, 10 ng/tube |
| EK0309-DA | Mouse BDNF Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.018 | 0.046 | 0.059 | 0.103 | 0.219 | 0.451 | 0.979 | 1.863 |
Data Example Images
Click image to see more details
Mouse BDNF PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Bdnf, Dilution ratio of 1:10, concentration in plasma is around 500 pg/ml..
Some articles we found to cite concentrations of Bdnf in samples: 24604632 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse BDNF ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 58 | 196 | 923 | 61 | 198 | 920 |
| Standard deviation | 4.06 | 14.5 | 46.15 | 5.42 | 18.21 | 60.72 |
| CV (%) | 7% | 7.4% | 5% | 8.9% | 9.2% | 6.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Bdnf in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 58 | 59 | 58 | 68 | 60 | 4.2 | 7% |
| Sample 2 | 196 | 221 | 215 | 235 | 216 | 14 | 6.4% |
| Sample 3 | 923 | 885 | 975 | 808 | 897 | 60.87 | 6.7% |
Protein Target Info & Infographic
Gene/Protein Information For BDNF (Source: Uniprot.Org, NCBI)
Gene Name
BDNF
Full Name
Brain-derived neurotrophic factor
Weight
27.818kDa
Superfamily
NGF-beta family
Alternative Names
Abrineurin; ANON2; BDNF; brain-derived neurotrophic factor; BULN2; MGC34632; Neurotrophin BDNF ANON2, BULN2 brain derived neurotrophic factor brain-derived neurotrophic factor|abrineurin|neurotrophin
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on BDNF, check out the BDNF Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for BDNF: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse BDNF ELISA Kit PicoKine® (EK0309)
Hello CJ!
EK0309 has been cited in 76 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Resveratrol reversed chronic restraint stress-induced impaired cognitive function in rats
Male C57BL6/N and C57BL6/J Mice Respond Differently to Constant Light and Running-Wheel Access
Chronic Hippocampal Abnormalities and Blunted HPA Axis in an Animal Model of Repeated Unpredictable Stress
Granulocyte-colony stimulating factor promotes brain repair following traumatic brain injury by recruitment of microglia and increasing neurotrophic factor expression
GT1b functions as a novel endogenous agonist of toll‐like receptor 2 inducing neuropathic pain
Neuropathy and neural plasticity in the subcutaneous white adipose depot
The involvement of neuroimmune cells in adipose innervation
Therapeutic potential of silymarin in chronic unpredictable mild stress induced depressive-like behavior in mice:
Expressions of Some Neurotrophins and Neurotrophic Cytokines at Site of Spinal Cord Injury in Mice after Vaccination with Dendritic Cells Pulsed with Homogenate Proteins
Brain‐derived neurotrophic factor fused with a collagen‐binding domain inhibits neuroinflammation and promotes neurological recovery of traumatic brain injury mice via TrkB signalling
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Customer Reviews
Have you used Mouse BDNF ELISA Kit PicoKine®?
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1 Reviews For Mouse BDNF ELISA Kit PicoKine®
0
It is a good kit with good quality.--JUNHUI WANG UNIVERSITY OF TORONTO PHYSIOLOGY RESEARCH ASSOCIATE
Excellent
Source: Biocompare.com
| Applications | ELISA |
|---|---|
| Sample | Brain Tissue Lysate |
| Detection | -- |
"I used the ELISA kit to detect the BDNF level in several samples, including brain tissues (cortex and hippocampus), cultured astrocyte (cell lysis) and astrocyte conditioned medium. The kit works very well for cell lysis and brain tissues according to the instruction. For the cell medium, I suggest that users need modify the protocol a little bit, such as prolong the incubation time for samples in the wells for overnight. The kit has a good quality."
Junhui Wang
Verified customer
Submitted 2019-05-24
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
18 Customer Q&As for Mouse BDNF ELISA Kit PicoKine®
Question
What do you think is the best method to prepare tissue samples using EK0309? What is the best and compatible lysis buffer for tissue homogenates to use with your ELISA Kit?
Verified customer
Asked: 2022-03-30
Answer
Mouse BDNF ELISA Kit PicoKine® (EK0309) and all our ELISA kits cannot detect tissue samples due to the presence of endogenous biotin. We use SABC for signal amplification and the endogenous biotin will result in high background.
Boster Scientific Support
Answered: 2022-04-08
Question
Does EK0309 recognize the total BDNF (free and Trk-bound) or just recognize the free BDNF?
Verified customer
Asked: 2022-03-23
Answer
Mouse BDNF ELISA Kit PicoKine® (EK0309) recognizes total BDNF.
Boster Scientific Support
Answered: 2022-03-24
Question
Is there an alternate wash buffer recipe for EK0309?
Verified customer
Asked: 2021-02-17
Answer
For the Mouse BDNF ELISA Kit PicoKine™ (EK0309), please see the following wash buffer recipe: Add 1.2g Tris, 8.5g Nacl, 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L. You can also contact our orders department to ask for extra wash buffer.
Boster Scientific Support
Answered: 2021-02-18
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification Bdnf using the EK0309 Mouse Bdnf Picokine® ELISA Kit?
K. Wu
Verified customer
Asked: 2020-11-05
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2020-11-05
Question
Q: is it okay to use citrate plasma as samples in Mouse Bdnf Picokine® ELISA Kit (Catalog # EK0309)?
Verified Customer
Verified customer
Asked: 2020-05-31
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of Bdnf causing degradation of its protein structure. Bdnf may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Bdnf ELISA, treating samples with various anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-05-31
Question
Is the sf21 expression system referring to insect cells? Keywords: insect cell line, immunogen, IPLB-Sf21-AE
Verified Customer
Verified customer
Asked: 2020-04-20
Answer
Yes, sf21 is an insect cell expression system.
Boster Scientific Support
Answered: 2020-04-20
Question
When choosing between lysis buffer and PBS to prepare tissue lysates, is there any benefit to using one over the other? Keywords: lysis buffer, TBS, PBS, washing buffer, lysate preparation, RIPA, ELISA
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
RIPA lysis buffer is the preferred buffer for lysate preparation because it lyses tissues more thoroughly than PBS.
Boster Scientific Support
Answered: 2020-03-31
Question
Does EK0309 measure all BDNF isoforms (pro and mature BDNF)? Keywords: isoform detection, pro BDNF, mature BDNF
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
EK0309 can measure both pro and mature BDNF. However, EK0309 cannot differentiate between pro BDNF and mature BDNF.
Boster Scientific Support
Answered: 2019-09-19
Question
Will using RIPA lysis system for lysate preparation have an effect on the ELISA Keywords: lysis buffer, TBS, PBS, washing buffer, lysate preparation, RIPA, ELISA
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
Using RIPA lysis system will not have any effect on the ELISA.
Boster Scientific Support
Answered: 2019-09-18
Question
Is it normal to have bubbles in the wells or the plate of EK0309? Will it affect the performance of the kit?
Verified customer
Asked: 2019-08-08
Answer
The bubbles would not affect the result of the Mouse BDNF ELISA Kit PicoKine™ (EK0309), Please wash the plate to remove the bubbles as suggested below prior to the assay. Wash the plate 3 times with the 1x wash buffer. a. Discard the liquid in the wells into an appropriate waste receptacle. Then, invert the plate on the benchtop onto a paper towel and tap the plate to gently blot any remaining liquid. It is recommended that the wells are not allowed to completely dry at any time. b. Add 300 µl of the 1x wash buffer to each assay well. (For cleaner background incubate for 60 seconds between each wash). c. Repeat steps a-b 2 additional times.
Boster Scientific Support
Answered: 2019-08-08
Question
Could cell lysate also be tested by this kit? What is your advice on how to prepare cell lysates? Keyword: application, sample, protocol
Verified Customer
Verified customer
Asked: 2019-06-07
Answer
We didn't test cell lysate, the kit can work if the concentration of BDNF in cell lysate is in the test range of the kit. The customer can have a try if he wants to detect it. The general preparation of cell lysate is as follows: Collect cells and rinse cells with PBS. Homogenize and lyse cells thoroughly in lysate solution(Cell Protein Extraction Reagent, Catalog# AR0103). Centrifuge cell lysates at approximately 10000 X g for 5 min to remove debris. Aliquots of the cell lysates were removed and assayed.
Boster Scientific Support
Answered: 2019-06-07
Question
Q: how to analyze ELISA data? I have obtained Bdnf level in cell lysates.
K. Wu
Verified customer
Asked: 2019-05-05
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-05-05
Question
Q: if the enzyme conjugated Bdnf antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Bdnf antigen?
V. Kapoor
Verified customer
Asked: 2019-02-13
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-02-13
Question
I am interested in performing Western Blot (WB) using the antibodies from your human and mouse BDNF ELISA kits. Is it possible to order the primary antibody (the capture antibody) that is pre-coated in the wells for the sandwich ELISA? (In this case that would be the monoclonal antibody from rat for the mouse kit, and the monoclonal antibody from mouse for the human kit). Is it also possible to order your secondary antibody which is the biotinylated detection polyclonal antibody from goat specific for BDNF?
Verified Customer
Verified customer
Asked: 2018-11-28
Answer
The antibodies used in those ELISA kits are not fully validated on WB. Also, since these antibodies are made from recombinant proteins, they are often less reactive to retrieved antigens in WB or IHC due to the lack of tertiary structure in the retrieved antigens. If you want to test human and mouse BDNF with WB, please use our anti-BDNF antibody (PB9075). Further, you can separately buy our secondary antibodies.
Boster Scientific Support
Answered: 2018-11-28
Question
Q: how to thaw whole blood sample for Bdnf ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-08-01
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Bdnf for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2018-08-01
Question
Is baculovirus used in the product of EK0309? Keyword: manufacturing
Verified Customer
Verified customer
Asked: 2018-05-29
Answer
Yes, baculovirus is used during the production of EK0309 as a transfection factor.
Boster Scientific Support
Answered: 2018-05-29
Question
Q: Can Bdnf ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2018-03-04
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-03-04
Question
I got very low OD values for the BDNF standard that you provided. What should I do?
Verified Customer
Verified customer
Asked: 2014-10-23
Answer
Try to repeat your experiment by extending your incubation times as follows: samples (150 min), biotinylated antibodies (70-80 min), ABC reagent (45 min) and TMB (30 min).
Boster Scientific Support
Answered: 2014-10-23
