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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate).|
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Mouse BDNF ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Mouse BDNF ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Bdnf in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate). Sensitivity: 15pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse BDNF ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0309)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: H129-R247
31.2 pg/ml - 2,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0309 is reactive to Bdnf in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate).
EK0309 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Bdnf
Brain-derived neurotrophic factor (BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor (NGF). BDNF and neurotrophin-3 (NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system. BDNF and other neurotrophins are critically involved in long-term potentiation (LTP). BDNF-mediated LTP is induced postsynaptically. BDNF has trophic effects on serotonergic (5-HT) neurons in the central nervous system. BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state. It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury. The mature form of BDNF is identical in all mammals examined, and the gene encoding human BDNF to chromosome 11, band p13.
|EK0309-CAP||Anti-Mouse Bdnf Pre-coated 96-well strip microplate||1|
|EK0309-ST||Mouse Bdnf Standard||2 vials, 10 ng/tube|
|EK0309-DA||Mouse Bdnf Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Mouse BDNF PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse BDNF ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Bdnf in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Bdnf (Source: Uniprot.Org, NCBI)
Brain-derived neurotrophic factor
Abrineurin; ANON2; BDNF; brain-derived neurotrophic factor; BULN2; MGC34632; Neurotrophin Bdnf|brain derived neurotrophic factor|brain-derived neurotrophic factor*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Bdnf, check out the Bdnf Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Bdnf: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0309 has been cited in 35 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Ferrimagnetic Nanochains‐Based Mesenchymal Stem Cell Engineering for Highly Efficient Post‐Stroke Recovery
Biney RP,Benneh CK,Adongo DW,Ameyaw EO,Woode E.Evidence of an antidepressant-like effect of xylopic acid mediated by serotonergic mechanisms.Psychopharmacology (Berl).2021 Apr 10.doi:10.1007/s00213-021-05835-6.Epub ahead of print.PMID:33837810.
EK0309 usage in article: APP:ELISA, SAMPLE:BRAIN TISSUE, DILUTION:NA
Thakare VN, Lakade SH, Mahajan MP, Kulkarni YP, Dhakane VD, Harde MT, Patel BM. Protocatechuic acid attenuates chronic unpredictable mild stress induced-behavioral and biochemical alterations in mice. Eur J Pharmacol. 2021 Mar 3;898:173992. doi: 10.1016/j.ejphar.2021.173992. Epub ahead of print. PMID: 33675783.
EK0309 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Apigenin reverses depression-like behavior induced by chronic corticosterone treatment in mice
Epigenetic modifications by inhibiting histone deacetylases reverse memory impairment in insulin resistance induced cognitive deficit in mice
Effect of mild hypothermia on angiogenesis in rats with focal cerebral ischemia
Histone deacetylase inhibitor, trichostatin A, improves learning and memory in high-fat diet-induced cognitive deficits in mice
Essential oil of Perilla frutescens-induced change in hippocampal expression of brain-derived neurotrophic factor in chronic unpredictable mild stress in mice
Circadian variations in behaviors, BDNF and cell proliferation in depressive mice
Transduced PTD-BDNF fusion protein protects against beta amyloid peptide-induced learning and memory deficits in mice
Physical exercise induces structural alterations in the hippocampal astrocytes: exploring the role of BDNF-TrkB signaling
Sexual dimorphism in BDNF signaling after neonatal hypoxia-ischemia and treatment with necrostatin-1
BDNF signaling is necessary for the antidepressant-like effect of naringenin.
Etazolate rescues behavioral deficits in chronic unpredictable mild stress model: modulation of hypothalamic-pituitary-adrenal axis activity and brain-derived neurotrophic factor level.
Therapeutic potential of silymarin in chronic unpredictable mild stress induced depressive-like behavior in mice.
Antidepressant-like effect of macranthol isolated from Illicium dunnianum tutch in mice.
Male C57BL6/N and C57BL6/J Mice Respond Differently to Constant Light and Running-Wheel Access.
Impact of Repetitive Mild Traumatic Brain Injury on Behavioral and Hippocampal Deficits in a Mouse Model of Chronic Stress
Neuroprotective role of Indirubin-3'-monoxime, a GSKβ inhibitor in high fat diet induced cognitive impairment in mice.
Neurotherapeutic Effect of Cord Blood Derived CD45 Hematopoietic Cells in Mice after Traumatic Brain Injury
Chronic Hippocampal Abnormalities and Blunted HPA Axis in an Animal Model of Repeated Unpredictable Stress
Ondansetron ameliorates depression associated with obesity in high-fat diet fed experimental mice: An investigation-based on the behavioral, biochemical, and molecular approach
The behavioral and physiological effects of high?fat diet and alcohol consumption: Sex differences in C57BL6/J mice
Magnetic Beads Enhance Adhesion of NIH 3T3 Fibroblasts: A Proof-of-Principle In Vitro Study for Implant-Mediated Long-Term Drug Delivery to the Inner Ear
Granulocyte colony-stimulating factor promotes behavioral recovery in a mouse model of traumatic brain injury
Fingolimod induces neurogenesis in adult mouse hippocampus and improves contextual fear memory
Differential Efficacy of Ketamine in the Acute versus Chronic Stages of Complex Regional Pain Syndrome in Mice.
The effects of EGb761 on lipopolysaccharide-induced depressive-like behaviour in C57BL/6J mice
Phosphodiesterase Type 4 Inhibitor Rolipram Improves Survival of Spiral Ganglion Neurons?In Vitro
Li M, Li C, Yu H, Cai X, Shen X, Sun X, Wang J, Zhang Y, Wang C. J Neuroinflammation. 2017 Sep 20;14(1):190. doi: 10.1186/s12974-017-0964-9. Lentivirus-mediated interleukin-1β (IL-1β) knock-down in the hippocampus alleviates lipopolysaccharide (LP...
Koh EJ, Kim KJ, Song JH, Choi J, Lee HY, Kang DH, Heo HJ, Lee BY. Int J Mol Sci. 2017 Nov 13;18(11). pii: E2401. doi: 10.3390/ijms18112401. Spirulina maxima Extract Ameliorates Learning and Memory Impairments via Inhibiting GSK-3β Phosphorylation ...
Zhang, Q., Wang, X., Bai, X., Xie, Y., Zhang, T., Bo, S., & Chen, X. (2017). Resveratrol reversed chronic restraint stress-induced impaired cognitive function in rats. Molecular Medicine Reports, 16(2), 2095-2100. Advance online publication. doi: ...
Schulze, J., Kaiser, O., Paasche, G., Lamm, H., Pich, A., Hoffmann, A.,..., & Warnecke, A. (2017). Effect of hyperbaric oxygen on BDNF-release and neuroprotection: Investigations with human mesenchymal stem cells and genetically modified NIH3T3 fi...
Chen, G., Ma, Q., Goswami, D., Shang, J., & Miller, G.M. (2017). Modulation of nuclear REST by alternative splicing: a potential therapeutic target for Huntington's disease. Journal of Cellular and Molecular Medicine. Advance online publication. d...
Tajerian M, Leu D, Zou Y, Sahbaie P, Li W, Khan H, Hsu V, Kingery W, Huang Tt, Becerra L, Clark Jd. Anesthesiology. 2014 Oct;121(4):852-65. Doi: 10.1097/Aln.0000000000000403. Brain Neuroplastic Changes Accompany Anxiety And Memory Deficits In A Mo...
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