Product Info Summary
SKU: | M07246 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human |
Host: | Mouse |
Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-ARHGEF4 Purified Monoclonal Antibody
SKU/Catalog Number
M07246
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ARHGEF4 Purified Monoclonal Antibody (Catalog# M07246). Tested in Flow Cytometry, WB application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-ARHGEF4 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07246)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
ARHGEF-08
Isotype
Mouse IgG1
Immunogen
Recombinant fragment of human ARHGEF4 (amino acids 143-271). The mouse monoclonal antibody ARHGEF-08 recognizes human intracellular protein ARHGEF4 / ASEF1, approx. 80 kDa guanine nucleotide exchange factor specific for Rac1 and Cdc42. The epitope is located in the rough at the region of SH3 domain.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
As in case with other anti-CD170 antibodies, this antibody cross-reacts with Siglec-14, whose first two Ig domains are almost identical to those of CD170.
Reactive Species
M07246 is reactive to ARHGEF4 in Human
Applications
M07246 is guaranteed for Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
79.067kDa
Background of ARHGEF4
ARHGEF4 (Rho guanine nucleotide exchange factor 4), also known as ASEF 1 (adenomatous polyposis coli –; stimulated guanine nucleotide exchange factor 1) is an approximately 80 kDa cytoplasmic protein important for growth factor-mediated regulation of cell morphology and migration. Besides N-terminal adenomatous polyposis coli (APC)-binding region (ABR) it contains Dbl homology (DH), pleckstrin homology (PH) and SH3 domains. The SH3 domain inhibits GEF activity of ARHGEF4 by intramolecular interaction with the DH domain, whereas binding of APC stimulates the GEF activity. Activated ARHGEF4 stimulates the small GTPase Cdc42, which leads to decreased cell-cell adherence and enhanced cell migration.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 μg/ml. Intracellular staining.
Validation Images & Assay Conditions
There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.
Protein Target Info & Infographic
Gene/Protein Information For ARHGEF4 (Source: Uniprot.org, NCBI)
Gene Name
ARHGEF4
Full Name
Rho guanine nucleotide exchange factor 4
Weight
79.067kDa
Alternative Names
APC-stimulated guanine nucleotide exchange factor 1; Asef; ASEF1; ASEFAPC-stimulated guanine nucleotide exchange factor; GEF4; KIAA1112; Rho guanine nucleotide exchange factor (GEF) 4; rho guanine nucleotide exchange factor 4; STM6 ARHGEF4 ASEF, ASEF1, GEF4, SMIM39, STM6 Rho guanine nucleotide exchange factor 4 rho guanine nucleotide exchange factor 4|APC-stimulated guanine nucleotide exchange factor 1|Rho guanine nucleotide exchange factor (GEF) 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ARHGEF4, check out the ARHGEF4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ARHGEF4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ARHGEF4 Purified Monoclonal Antibody (M07246)
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No publications found for M07246
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