Anti-ARHGEF4 Purified Monoclonal Antibody

Product Name

Anti-ARHGEF4 Purified Monoclonal Antibody

View all ARHGEF4 Antibodies

SKU/Catalog Number

M07246

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-ARHGEF4 Purified Monoclonal Antibody (Catalog# M07246). Tested in Flow Cytometry, WB application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-ARHGEF4 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07246)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

ARHGEF-08

Isotype

Mouse IgG1

Immunogen

Recombinant fragment of human ARHGEF4 (amino acids 143-271). The mouse monoclonal antibody ARHGEF-08 recognizes human intracellular protein ARHGEF4 / ASEF1, approx. 80 kDa guanine nucleotide exchange factor specific for Rac1 and Cdc42. The epitope is located in the rough at the region of SH3 domain.

Cross-reactivity

As in case with other anti-CD170 antibodies, this antibody cross-reacts with Siglec-14, whose first two Ig domains are almost identical to those of CD170.

Reactive Species

M07246 is reactive to ARHGEF4 in Human

Observed Molecular Weight

42 kDa

Calculated molecular weight

79.1 kDa

Background of ARHGEF4

ARHGEF4 (Rho guanine nucleotide exchange factor 4), also known as ASEF 1 (adenomatous polyposis coli –; stimulated guanine nucleotide exchange factor 1) is an approximately 80 kDa cytoplasmic protein important for growth factor-mediated regulation of cell morphology and migration. Besides N-terminal adenomatous polyposis coli (APC)-binding region (ABR) it contains Dbl homology (DH), pleckstrin homology (PH) and SH3 domains. The SH3 domain inhibits GEF activity of ARHGEF4 by intramolecular interaction with the DH domain, whereas binding of APC stimulates the GEF activity. Activated ARHGEF4 stimulates the small GTPase Cdc42, which leads to decreased cell-cell adherence and enhanced cell migration.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M07246 is guaranteed for Flow Cytometry, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: 1-4 μg/ml. Intracellular staining.

Validation Images & Assay Conditions

See full target information ARHGEF4

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