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Product Info Summary
| SKU: | A02405 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Goat |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Artemis DCLRE1C Antibody
SKU/Catalog Number
A02405
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Artemis DCLRE1C Antibody (Catalog # A02405). Tested in ELISA, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Artemis DCLRE1C Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02405)
Host
Goat
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding an internal region of Human ARTEMIS (DCLRE1C DNA cross-link repair 1C).
Reactive Species
A02405 is reactive to DCLRE1C in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
78.4 kDa
Background of DCLRE1C
Artemis has a role in T and B lymphocyte immunodeficiency and in predisposition to lymphoma through the NHEJ pathway of DNA repair. DNA-PKcs regulates Artemis by both phosphorylation and complex formation to permit enzymatic activities that are critical for the hairpin-opening step of V(D)J recombination and for the 5' and 3' overhang processing in non-homologous DNA end joining.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02405 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:10,000 - 1:20,000
IHC: 1:1,000
WB: 1:500 - 1:2,000
This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blotting. Reactivity in other immunoassays is unknown. This antibody detects a band of approximately 90 kDa (predicted molecular weight: 78 kDa).
Validation Images & Assay Conditions
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Boster's Affinity Purified anti-Artemis antibody was used at a 1:1000 dilution to detect Artemis by immunohistochemistry in human spleen. Positive staining of T cells and B lymphocytes is observed in thymus, lymph nodes and spleen. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Alan Yen, LifeSpanBiosciences, Seattle, WA.
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Western blot using Boster's Goat-anti-Artemis was used to detect Artemis. Lane 1: AT7BI cell lysates. Lane 2: MO59K cell lysates. Lane 3: MO59J cell lysates. Lane 4: HeLa cell lysates . Lane 5: CJ cell lysates. CJ is an Artemis deficient cell line and so no band is visible. Primary Antibody at 1:500 dilution. Signal was detected using standard techniques.
Specific Publications For Anti-Artemis DCLRE1C Antibody (A02405)
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Customer Q&As
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7 Customer Q&As for Anti-Artemis DCLRE1C Antibody
Question
Is a blocking peptide available for product anti-Artemis antibody (A02405)?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
We do provide the blocking peptide for product anti-Artemis antibody (A02405). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-01-24
Question
We want to test anti-Artemis antibody A02405 on human cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-Artemis antibody A02405 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-23
Answer
The products we sell, including anti-Artemis antibody A02405, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-23
Question
Will A02405 anti-Artemis antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Z. Roberts
Verified customer
Asked: 2018-06-15
Answer
You can see on the product datasheet, A02405 anti-Artemis antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-06-15
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-Artemis antibody A02405. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-03-22
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-03-22
Question
See below the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-Artemis antibody A02405. Please let me know if you require anything else.
A. Krishna
Verified customer
Asked: 2016-07-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-07-13
Question
Does anti-Artemis antibody A02405 work on bovine WB with embryo?
L. Bhatt
Verified customer
Asked: 2013-12-09
Answer
Our lab technicians have not tested anti-Artemis antibody A02405 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-Artemis antibody A02405 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine embryo in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-12-09
Question
We are currently using anti-Artemis antibody A02405 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on feline tissues as well?
A. Brown
Verified customer
Asked: 2013-02-22
Answer
The anti-Artemis antibody (A02405) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-02-22
