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Product Info Summary
| SKU: | M13193 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-ATP6V1C2 Mouse Monoclonal Antibody [Clone ID: OTI8H4]
SKU/Catalog Number
M13193
Size
100 µl
Description
Boster Bio ATP6V1C2 mouse monoclonal antibody, clone OTI8H4 (formerly 8H4). Catalog# M13193. Tested in IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-ATP6V1C2 Mouse Monoclonal Antibody [Clone ID: OTI8H4] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M13193)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI8H4
Isotype
IgG1
Immunogen
Full length human recombinant protein of human ATP6V1C2 (NP_653184) produced in E.coli.
Reactive Species
M13193 is reactive to ATP6V1C2 in Human, Mouse, Rat
Calculated molecular weight
48.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M13193 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:2000
IHC 1:150
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP6V1C2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP6V1C2 (1:2000).
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ATP6V1C2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ATP6V1C2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-ATP6V1C2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ATP6V1C2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ATP6V1C2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-ATP6V1C2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
Specific Publications For Anti-ATP6V1C2 Mouse Monoclonal Antibody [Clone ID: OTI8H4] (M13193)
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