Product Info Summary
| SKU: | A03381 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Autophagy-related protein 9B ATG9B Antibody
SKU/Catalog Number
A03381
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Autophagy-related protein 9B ATG9B Antibody (Catalog # A03381). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
ATG9B antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Autophagy-related protein 9B ATG9B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03381)
Host
Rabbit
Contents
ATG9B Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ATG9B antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human ATG9B. The immunogen is located within amino acids 850 - 900 of ATG9B.
Reactive Species
A03381 is reactive to ATG9B in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
101.0 kDa
Background of ATG9B
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG9B plays a role in autophagy and it's highly expressed in placenta and pituitary gland.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03381 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ATG9B antibody can be used for detection of ATG9B by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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WB Validation in Human Tonsil Loading: 15 μg of human tonsil lysate. Antibodies: ATG9B A03381, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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Immunocytochemistry of ATG9B in HeLa cells with ATG9B antibody at 10 μg/mL.
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Immunofluorescence of ATG9B in Hela cells with ATG9B antibody at 20 μg/mL.
Specific Publications For Anti-Autophagy-related protein 9B ATG9B Antibody (A03381)
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