Product Info Summary
SKU: | A03381 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Autophagy-related protein 9B ATG9B Antibody
SKU/Catalog Number
A03381
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Autophagy-related protein 9B ATG9B Antibody (Catalog # A03381). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
ATG9B antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Autophagy-related protein 9B ATG9B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03381)
Host
Rabbit
Contents
ATG9B Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ATG9B antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human ATG9B. The immunogen is located within amino acids 850 - 900 of ATG9B.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least three isoforms of TET3 are known to exist. TET3 antibody is predicted to not cross react with other TET proteins.
Reactive Species
A03381 is reactive to ATG9B in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
101019 MW
Background of Atg9b
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG9B plays a role in autophagy and it's highly expressed in placenta and pituitary gland.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03381 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ATG9B antibody can be used for detection of ATG9B by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ATG9B in HeLa cell lysate with ATG9B antibody at (A) 1 and (B) 2 μg/mL.
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Immunocytochemistry of ATG9B in HeLa cells with ATG9B antibody at 10 μg/mL.
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Immunofluorescence of ATG9B in Hela cells with ATG9B antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For ATG9B (Source: Uniprot.org, NCBI)
Gene Name
ATG9B
Full Name
Autophagy-related protein 9B
Weight
101019 MW
Superfamily
ATG9 family
Alternative Names
SONE, APG9L2, NOS3AS, Autophagy-related protein 9B, APG9-like 2, Protein sONE Atg9b|Apg, Apg912, Apg9l2, Apgdc2, Gm574, N, Nos3as, eO, eONE, sONE|autophagy related 9B|autophagy-related protein 9B|APG9-like 2|ATG9 autophagy related 9 homolog B|autophagy 9-like 2 protein|nitric oxide synthase 3 antisense|nitric oxide synthase 3-overlapping antisense gene protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATG9B, check out the ATG9B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATG9B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Autophagy-related protein 9B ATG9B Antibody (A03381)
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