Anti-BAFF Receptor/TNFRSF13C Antibody
Rabbit IgG polyclonal antibody for Tumor necrosis factor receptor superfamily member 13C(TNFRSF13C) detection. Tested with WB in Human.
Anti-BAFF Receptor/TNFRSF13C Antibody Info At A Glance
|Product Name||Anti-BAFF Receptor/TNFRSF13C Antibody
See all BAFFR/TNFRSF13C primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for BAFF R/TNFRSF13C detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. BAFF R/TNFRSF13C information: Molecular Weight: 18864 MW; Subcellular Localization: Membrane ; Single-pass type III membrane protein ; Tissue Specificity: Highly expressed in spleen and lymph node, and in resting B-cells. Detected at lower levels in activated B-cells, resting CD4+ T-cells, in thymus and peripheral blood leukocytes.|
|Cite This Product||Anti-BAFF Receptor/TNFRSF13C Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1391)|
|Similar Products From
|Anti-BAFF Receptor/TNFRSF13C Antibody may replace the following items: sc 242365|sc 242366|sc 242367|sc 398915.|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human BAFF Receptor(1-15aa MRRGPRSLRGRDAPA).|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For TNFRSF13C (Source: Uniprot.org, NCBI)
|NCBI Gene Id||115650|
|Species Of This Entry||Human|
|Protein Name||Tumor necrosis factor receptor superfamily member 13C|
|Alternative Names||BAFFR/TNFRSF13C|BAFF R; BAFFR; BR3; CD268; TNFRSF13C|
|Post Tranlational Modifications||Phosphorylation; Cleavage; Ubiquitination|
|Subcellular Localization||Membrane; Single-pass type III membrane protein.|
|Gene Location||22q13.2, on Chromasome 22, gene sequence: NC_000022.11 (41922032..41926806, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For TNFRSF13C (Source: Uniprot.org, NCBI)
|Protein Function||B-cell receptor specific for TNFSF13B/TALL1/BAFF/BLyS. Promotes the survival of mature B-cells and the B-cell response.|
|Tissue Specificity||Highly expressed in spleen and lymph node, and in resting B-cells. Detected at lower levels in activated B-cells, resting CD4+ T-cells, in thymus and peripheral blood leukocytes.|
|Related Diseases||Autoimmune Diseases; Autoimmune Reaction; Autoimmunity; Lymphoma; Lupus Erythematosus, Systemic; B-cell Lymphomas; Malignant Neoplasms; Neoplasms; Immunologic Deficiency Syndromes; Malignant Paraganglionic Neoplasm; Rheumatoid Arthritis; Common Variable Immunodeficiency; Multiple Myeloma; Arthritis; Inflammation; Diffuse Large B-cell Lymphoma; Hyperplasia; Lymphoma, Non-hodgkin; Leukemia; Chronic Lymphocytic Leukemia|
|Related Pathways||Cell Maturation; Cell Development; Pathogenesis; Cell Activation; B Cell Activation; Cell Proliferation; Secretion; Cell Growth; B Cell Proliferation; B Cell Homeostasis; Cell Differentiation; B Cell Differentiation; T Cell Activation; Immune Response; B Cell Selection; Localization; Cell Death; Isotype Switching; Immunoglobulin Production; Humoral Immune Response|
|Background||Tumor necrosis factor receptor superfamily member 13C(TNFRSF13C), also known as BAFFR, is a protein in humans is encoded by the TNFRSF13C gene. The BAFFR gene is mapped to chromosome 22q13.1-q13.31. It has got 184 amino acid transmembrane protein which is 56% identical to the mouse protein. B cell-activating factor(BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. BAFF plays a crucial role in B cell development and can function through receptors other than BCMA.|
|Scientific References||PMID: 11509692 by Thompson J.S., et al. BAFF-R, a newly identified TNF receptor that specifically interacts with BAFF.|
PMID: 11591325 by Yan M., et al. Identification of a novel receptor for B lymphocyte stimulator that is mutated in a mouse strain with severe B cell deficiency.
Our Boster Quality Guarantee for Anti-BAFF Receptor/TNFRSF13C Antibody covers its use in the following applications.Western blot, 0.1-0.5μg/ml, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates,
Lane 2: human HEK293 whole cell lysates,
Lane 3: human K562 whole cell lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- BAFFR antigen affinity purified polyclonal antibody (Catalog # PA1391) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BAFFR at approximately 40KD. The expected band size for BAFFR is at 19KD.
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