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Product Info Summary
| SKU: | PA1391 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-BAFF Receptor/TNFRSF13C Antibody Picoband®
SKU/Catalog Number
PA1391
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-BAFF Receptor/TNFRSF13C Antibody catalog # PA1391. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BAFF Receptor/TNFRSF13C Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1391)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human BAFF Receptor.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1391 is reactive to TNFRSF13C in Human
Observed Molecular Weight
40 kDa
Calculated molecular weight
18.9 kDa
Background of TNFRSF13C
Tumor necrosis factor receptor superfamily member 13C (TNFRSF13C), also known as BAFFR, is a protein in humans is encoded by the TNFRSF13C gene. The BAFFR gene is mapped to chromosome 22q13.1-q13.31. It has got 184 amino acid transmembrane protein which is 56% identical to the mouse protein. B cell-activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. BAFF plays a crucial role in B cell development and can function through receptors other than BCMA.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1391 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human Raji whole cell, human HEK293 whole cell, human K562 whole cell
Validation Images & Assay Conditions
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Western blot analysis of BAFFR using anti-BAFFR antibody (PA1391).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates,
Lane 2: human HEK293 whole cell lysates,
Lane 3: human K562 whole cell lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAFFR antigen affinity purified polyclonal antibody (Catalog # PA1391) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BAFFR at approximately 40KD. The expected band size for BAFFR is at 19KD.
Specific Publications For Anti-BAFF Receptor/TNFRSF13C Antibody Picoband® (PA1391)
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5 Customer Q&As for Anti-BAFF Receptor/TNFRSF13C Antibody Picoband®
Question
Does anti-BAFF Receptor/TNFRSF13C antibody PA1391 work for WB with tonsil?
Verified Customer
Verified customer
Asked: 2020-03-10
Answer
According to the expression profile of tonsil, TNFRSF13C is highly expressed in tonsil. So, it is likely that anti-BAFF Receptor/TNFRSF13C antibody PA1391 will work for WB with tonsil.
Boster Scientific Support
Answered: 2020-03-10
Question
We are currently using anti-BAFF Receptor/TNFRSF13C antibody PA1391 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
The anti-BAFF Receptor/TNFRSF13C antibody (PA1391) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-12
Question
Is a blocking peptide available for product anti-BAFF Receptor/TNFRSF13C antibody (PA1391)?
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
We do provide the blocking peptide for product anti-BAFF Receptor/TNFRSF13C antibody (PA1391). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-10-29
Question
Does anti-BAFF Receptor/TNFRSF13C antibody PA1391 work on canine WB with tonsil?
Verified Customer
Verified customer
Asked: 2019-01-11
Answer
Our lab technicians have not validated anti-BAFF Receptor/TNFRSF13C antibody PA1391 on canine. You can run a BLAST between canine and the immunogen sequence of anti-BAFF Receptor/TNFRSF13C antibody PA1391 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine tonsil in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-01-11
Question
I have a question about product PA1391, anti-BAFF Receptor/TNFRSF13C antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
T. Bhatt
Verified customer
Asked: 2018-05-04
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1391 anti-BAFF Receptor/TNFRSF13C antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-05-04
