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Product Info Summary
|Reactive Species:||Mouse, Rat|
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Anti-BAFF Receptor/Tnfrsf13c Antibody Picoband™
Boster Bio Anti-BAFF Receptor/Tnfrsf13c Antibody Picoband™ catalog # A02865-2. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BAFF Receptor/Tnfrsf13c Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02865-2)
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E. coli-derived mouse BAFF Receptor recombinant protein (Position: M1-A71).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A02865-2 is reactive to Tnfrsf13c in Mouse, Rat
A02865-2 is guaranteed for ELISA, WB Boster Guarantee
Background of BAFFR/TNFRSF13C
Tumor necrosis factor receptor superfamily member 13C (TNFRSF13C), also known as BAFFR, is a protein in humans is encoded by the TNFRSF13C gene. The BAFFR gene is mapped to chromosome 22q13.1-q13.31. It has got 184 amino acid transmembrane protein which is 56% identical to the mouse protein. B cell-activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. BAFF plays a crucial role in B cell development and can function through receptors other than BCMA.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of BAFF Receptor using anti-BAFF Receptor antibody (A02865-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: rat thymus tissue lysates,
Lane 3: mouse spleen tissue lysates,
Lane 4: mouse thymus tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAFF Receptor antigen affinity purified polyclonal antibody (Catalog # A02865-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BAFF Receptor at approximately 19, 40KD. The expected band size for BAFF Receptor is at 19KD.
Gene/Protein Information For Tnfrsf13c (Source: Uniprot.Org, NCBI)
Tumor necrosis factor receptor superfamily member 13C
BAFF R; BAFFR; BR3; CD268; TNFRSF13C Tnfrsf13c|2010006P15Rik, BAFF, BAFF-R, Baf, Baffr, Bcmd, Bcmd-1, Bcmd1, Lv, Lvis22|tumor necrosis factor receptor superfamily, member 13c|tumor necrosis factor receptor superfamily member 13C|B-cell maturation defect 1|B-cell-activating factor receptor|BAFF receptor|BLyS receptor 3|b cell-activating factor receptor*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Tnfrsf13c, check out the Tnfrsf13c Infographic
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In this infographic you will see the following information for Tnfrsf13c: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for A02865-2
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