|Validated Species:||Human, Mouse, Rat|
|Application:||IHC, ICC, WB|
Data & Images
|Product Name||Anti-BAK Antibody|
|Description||Rabbit IgG polyclonal antibody for Bcl-2 homologous antagonist/killer(BAK1) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.|
|Cite This Product||Anti-BAK Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1437)|
|Replacement Item||This antibody may replace the following items: sc-1035|sc-7873|sc-832|sc-832-G from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
|Application||IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human BAK1(74-95aa QVGRQLALIGDDINRRYDSEFQ), different from the related mouse and rat sequences by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Bcl-2 homologous antagonist/killer|
|Molecular Weight||23409 MW|
|Protein Function||In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti- apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis. .|
|Tissue Specificity||Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle.|
|Sequence Similarities||Belongs to the Bcl-2 family.|
|Subcellular Localization||Mitochondrion membrane ; Single-pass membrane protein .|
|Alternative Names||Bcl-2 homologous antagonist/killer;Apoptosis regulator BAK;Bcl-2-like protein 7;Bcl2-L-7;BAK1;BAK, BCL2L7, CDN1;|
|Research Areas|||cell biology|apoptosis|intracellular|bcl2 family||
Background for Bcl-2 homologous antagonist/killer
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-BAK Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunocytochemistry , 0.5-1μg/ml, Human, -|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-BAK Antibody Images
Click the images to enlarge.
All lanes: Anti BAK (PA1437) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Predicted bind size: 23KD
Observed bind size: 23KD
IHC(P): Rat Lung Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,