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Product Info Summary
| SKU: | PB9009 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Progesterone Receptor/PGR Antibody Picoband®
SKU/Catalog Number
PB9009
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Progesterone Receptor/PGR Antibody Picoband® catalog # PB9009. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Progesterone Receptor/PGR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9009)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Progesterone Receptor recombinant protein (Position: M595-K933). Human Progesterone Receptor shares 95% and 94% amino acid (aa) sequences identity with mouse and rat Progesterone Receptor, respectively.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9009 is reactive to PGR in Human
Observed Molecular Weight
90 kDa (PR-A), 118 kDa (PR-B)
Calculated molecular weight
99.0 kDa
Background of PGR
The progesterone receptor (PR) is an intracellular steroid receptor that specially binds progesterone in humans. PR has been a member of the steroid receptor superfamily. It is encoded by a single PGR gene residing on chromosome 11q22. The PGR gene uses separate promoters and translational start sites to produce 2 isoforms, PRA and PRB, which are identical except for an additional 165 amino acids present only in the N terminus of PRB. It can be observed in human breast tissues. The proteins function as dimeric molecules in nuclei to regulate the transcription of target genes in a ligand-responsive manner.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9009 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Positive Control
WB: human T-47D whole cell,
IHC: human mammary cancer tissue
ICC/IF: T-47D cell
Validation Images & Assay Conditions
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IHC analysis of Progesterone Receptor using anti-Progesterone Receptor antibody (PB9009).
Progesterone Receptor was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Progesterone Receptor Antibody (PB9009) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Western blot analysis of Progesterone Receptor using anti-Progesterone Receptor antibody (PB9009).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human T-47D whole cell lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Progesterone Receptor antigen affinity purified polyclonal antibody (Catalog # PB9009) at 0.1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Progesterone Receptor at approximately 90 KD (PR-A), 118KD (PR-B). The expected band size for Progesterone Receptor is at 99KD.
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IF analysis of Progesterone Receptor using anti-Progesterone Receptor antibody (PB9009).
Progesterone Receptor was detected in immunocytochemical section of T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-Progesterone Receptor Antibody (PB9009) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Specific Publications For Anti-Progesterone Receptor/PGR Antibody Picoband® (PB9009)
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3 Customer Q&As for Anti-Progesterone Receptor/PGR Antibody Picoband®
Question
Our customer is considering this antibody for their experiments. However, they noticed that on the WB image, it detects only 1 band. As PR has at least two common isoforms, A and B, and this is a polyclonal Ab with immunogen common to both isoforms, they would like to see if you have any testing data on isoform cross-reactivity. Could you please kindly advise on this?
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
Please see the updated result image on the product page. https://www.bosterbio.com/anti-progesterone-receptor-picoband-trade-antibody-pb9009-boster.html?options=cart It detects two bands.
Boster Scientific Support
Answered: 2020-04-01
Question
We are currently using anti-Progesterone Receptor/PGR antibody PB9009 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
The anti-Progesterone Receptor/PGR antibody (PB9009) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-21
Question
I am interested in your PB9009 Progesterone Receptor antibody. Have you tested the antibody with mouse and rat samples by WB and IHC?
Verified Customer
Verified customer
Asked: 2017-12-26
Answer
Our lab technicians tested rat sampels for IHC and failed to get positive signal. We haven't got available appropriate mouse and rat samples for WB test.
Boster Scientific Support
Answered: 2017-12-26
