Product Info Summary
| SKU: | A11769 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-BAP29 BCAP29 Antibody
SKU/Catalog Number
A11769
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-BAP29 BCAP29 Antibody (Catalog # A11769). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
BAP29 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-BAP29 BCAP29 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11769)
Host
Rabbit
Contents
BAP29 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
BAP29 antibody was raised against a 17 amino acid synthetic peptide from near the center of human Bap29 Bap29 antibody is specific for isoform a of Bap29. The immunogen is located within amino acids 130 - 180 of BAP29.
Reactive Species
A11769 is reactive to BCAP29 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
28.3 kDa
Background of BCAP29
Bap29 and the related protein Bap31 are endoplasmic reticulum (ER) and ER-vesicle membrane proteins and members of the B-cell receptor-associated protein family. These two proteins are highly homologous and can form homo- and heterodimers. Both Bap29 and Bap31 interact with membrane-bound immunoglobulins (mIgs), such as IgM and IgD, which with Ig-alpha/Ig-beta heterodimers form B cell antigen receptors. Binding of the Bap29/Bap31 heterodimer correlates with the ER retention of non-Ig-alpha/Ig-beta bound mIg complexes, suggesting that Bap29 and Bap31 may act as chaperones transmembrane regions of various proteins. Bap29 possesses multiple isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11769 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Bap29 antibody can be used for the detection of Bap29 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of Bap29 in human heart tissue lysate with Bap29 antibody at (A) 0.5, (B) 1 and (C) 2μg/mL.
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Immunohistochemistry of BAP29 in human heart tissue with BAP29 antibody at 10 μg/mL.
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Immunofluorescence of BAP29 in Human Heart cells with BAP29 antibody at 20 μg/mL.
Specific Publications For Anti-BAP29 BCAP29 Antibody (A11769)
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